LOCUS       sequence-326348-        4097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the original mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Valbuena FM, Fizgerald I, Strack RL, Andruska N, Smith L, Glick BS
  TITLE     A photostable monomeric superfolder GFP.
  JOURNAL   Traffic. 2020 May 15. doi: 10.1111/tra.12737.
  PUBMED    32415747
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2020 May 15. doi: 10.1111/tra.12737."
COMMENT     Sequence Label: pQE-60NA-mScarlet-I
FEATURES             Location/Qualifiers
     source          1..4097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     156..174
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(198..212,213..218,219..235,236..242)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    218..234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    250..266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             285..296
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             303..998
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(436..454)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     704..723
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1032..1126
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1236..1255)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1898..1984
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2031..2053)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2139..2279
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2194..2213
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2294..2311)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2465..3053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2545..2564)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3224..4015,4016..4084))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3847..3866
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(4085..4097,1..92))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
       61 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgct
      121 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
      181 gtcttcacct cgagaaatca taaaaaattt atttgctttg tgagcggata acaattataa
      241 tagattcaat tgtgagcgga taacaatttc acacagaatt cattaaagag gagaaattaa
      301 ccatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg
      361 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      421 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg
      481 acatcctgtc ccctcagttc atgtacggct ccagggcctt catcaagcac cccgccgaca
      541 tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      601 tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga
      661 tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga
      721 agacaatggg ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg
      781 gcgacattaa gatggccctg cgcctgaagg acggcggccg ctacctggcg gacttcaaga
      841 ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc gaccgcaagt
      901 tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg
      961 gccgccactc caccggcggc atggacgagc tgtacaagta aagcggccgc aagcttaatt
     1021 agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
     1081 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gctagcttgg
     1141 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     1201 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     1261 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaaggcc gtaaagaaaa
     1321 ataagcacaa gttttgtccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     1381 cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     1441 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     1501 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     1561 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     1621 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     1681 tcaccatgca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     1741 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1801 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
     1861 taaacgcctg gggtaatgac tctctagctt gaggcatcaa ataaaacgaa aggctcagtc
     1921 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     1981 aaatccgccc tctagagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2041 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2101 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     2161 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     2221 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     2281 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2341 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2401 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2461 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2521 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2581 tgcgccctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2641 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2701 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2761 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2821 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2881 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2941 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3001 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3061 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3121 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3181 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3241 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3301 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3361 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3421 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3481 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3541 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3601 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3661 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3721 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3781 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3841 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3901 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3961 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4021 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4081 tcatactctt ccttttt
//
