LOCUS       sequence-326349-        9718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the HIF3A transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0002
FEATURES             Location/Qualifiers
     source          1..9718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2656..3244
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2709..2729)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3127..3138)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3316..3549
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3627..3748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3664..3683)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3718..3737
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3788..3923
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3943..3962)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3944..3962)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3972..3989)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3972..3988)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3981..4003)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4130..4585
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4217..4236)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4427..4448
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4611..4715
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4716..4784,4785..5576)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4934..4953)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5747..6335
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6236..6255
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6489..6506
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6623..6644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6659..6664,6665..6682,6683..6689)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6697..6713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6702..6724
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6721..6737
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6721..6737
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6756..6776
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6758..6776
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6804..7030
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7031..7211
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7258..7383
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7876..8109
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8294..8338
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8569..8779
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8591..8787
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8638..8773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8819..9421
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8819..8838)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9315..9335
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9464..9581
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgcg
     1201 gcccgcagcc ggcgcagcca ggagaccgag gtgctgtacc agctggctca cacgctgccc
     1261 ttcgcccgcg gcgtcagcgc ccacctggac aaggcctcta tcatgcgcct caccatcagc
     1321 tacctgcgca tgcaccgcct ctgcgccgca gctggagctc attggacaca gcatctttga
     1381 tttcatccac ccctgtgacc aagaggagct tcaggacgcc ctgacccccc agcagaccct
     1441 gtccaggagg aaggtggagg cccccacgga gcggtgcttc tccttgcgca tgaagagtac
     1501 actcaccagc cgcgggcgca ccctcaacct caaggcggcc acctggaagg tgctgaactg
     1561 ctctggacat atgagggcct acaagccacc tgcgcagact tctccagctg ggagccctga
     1621 ctcagagccc ccgctgcagt gcctggtgct catctgcgaa gccatccccc acccaggcag
     1681 cctggagccc ccactgggcc gaggggcctt cctcagccgc cacagcctgg acatgaagtt
     1741 cacctactgt gacgacagga ttgcagaagt ggctggctat agtcccgatg acctgatcgg
     1801 ctgttccgcc tacgagtaca tccacgcgct ggactccgat gcggtcagca agagcatcca
     1861 caccttgctg agcaagggcc aggcagtaac agggcagtat cgcttcctgg cccggagtgg
     1921 tggctacctg tggacccaga cccaggccac agtggtgtca gggggacggg gcccccagtc
     1981 ggagagtatc gtctgtgtcc attttttaat cagccaggtg gaagagaccg gagtggtgct
     2041 gtccctggag caaacggagc aacactctcg cagacccatt cagcggggcg ccccctctca
     2101 gaaggacacc cctaaccctg gggacagcct tgacacccct ggcccccgga tccttgcctt
     2161 cctgcacccg ccttccctga gcgaggctgc cctggccgct gacccccgcc gtttctgcag
     2221 ccctgacctc cgtcgcctcc tgggacccat cctggatggg gcttcagtag cagccactcc
     2281 cagcaccccg ctggccacac ggcaccccca aagtcctctt tcggctgatc tcccagatga
     2341 actacctgtg ggcaccgaga atgtgcacag actcttcacc tccgggaaag acactgaggc
     2401 agtggagaca gatttagata tagctcagga ccccagcacc ccactcctga acctgaatga
     2461 gcccctgggt tttcactttg tcacccagtc tggagtgcag tggcacaaac acagctcacc
     2521 gcagcctcga cctcctgggc tcaagtagta atgaactagt ccgtaacttg aaagtatttc
     2581 gatttcttgg ctttatatat cttgtggaaa ggacgacaat tacaccacgt ctgcctacta
     2641 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2701 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2761 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2821 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2881 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2941 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3001 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3061 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3121 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3181 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3241 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3301 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     3361 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3421 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3481 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3541 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     3601 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     3661 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3721 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     3781 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3841 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3901 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     3961 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4021 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4081 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4141 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4201 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4261 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4321 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4381 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4441 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4501 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4561 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4621 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4681 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4741 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4801 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4861 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4921 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4981 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5041 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5101 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5161 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5221 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5281 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5341 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5401 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5461 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5521 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5581 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5641 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5701 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5761 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5821 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5881 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5941 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6001 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6061 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6121 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6181 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6241 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6301 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6361 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6421 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6481 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6541 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6601 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6661 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6721 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     6781 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     6841 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     6901 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     6961 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     7021 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7081 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7141 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7201 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7261 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7321 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7381 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7441 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     7501 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     7561 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     7621 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     7681 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     7741 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     7801 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     7861 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     7921 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     7981 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     8041 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8101 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8161 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8221 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8281 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8341 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     8401 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     8461 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg
     8521 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     8581 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8641 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8701 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8761 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat
     8821 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     8881 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     8941 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     9001 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
     9061 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     9121 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     9181 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     9241 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     9301 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     9361 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta
     9421 acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     9481 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     9541 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     9601 gcagagatcc actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta
     9661 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaatt
//
