LOCUS       sequence-326353-       12196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the PRDM16 transcription factor
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0675
FEATURES             Location/Qualifiers
     source          1..12196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3939..4527
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3992..4012)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4410..4421)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4599..4832
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4910..5031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4947..4966)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5001..5020
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5071..5206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5226..5245)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5227..5245)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5255..5272)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5255..5271)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5264..5286)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5413..5868
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5500..5519)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5710..5731
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5894..5998
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5999..6067,6068..6859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6217..6236)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7030..7618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7519..7538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7772..7789
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7906..7927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7942..7947,7948..7965,7966..7972)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7980..7996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7985..8007
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8004..8020
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8004..8020
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8039..8059
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8041..8059
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8087..8313
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8314..8494
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8541..8666
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9159..9392
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9577..9621
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9852..10062
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9874..10070
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9921..10056
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10102..10704
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10102..10121)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10598..10618
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10747..10864
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        11002..12180
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          11233..12171
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     12128..12148
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 atgcgatcca aggcgagggc gaggaagcta gccaaaagtg acggtgacgt tgtaaataat
       61 atgtatgagc ccaaccggga cctgctggcc agccacagcg cggaggacga ggccgaggac
      121 agtgccatgt cgcccatccc cgtggggcca ccgtccccct tccccaccag cgaggacttc
      181 acccccaagg agggctcgcc gtacgaggcc cctgtctaca ttcctgaaga cattccgatc
      241 ccagcagact tcgagctccg agagtcctcc atcccagggg ctggcctggg ggtctgggcc
      301 aagaggaaga tggaagccgg ggagaggctg ggcccctgcg tggtggtgcc ccgggcggcg
      361 gcaaaggaga cagacttcgg atgggagcaa atactgacgg acgtggaagt gtcgccccag
      421 gaaggctgca tcacaaagat ctccgaagac ctgggcagtg agaagttctg cgtggatgca
      481 aatcaggcgg gggctggcag ctggctcaag tacatccgtg tggcgtgctc ctgcgatgac
      541 cagaacctca ccatgtgtca gatcagtgag cagatttact ataaagtcat taaggacatt
      601 gagccaggtg aggagctgct ggtgcacgtg aaggaaggcg tctaccccct gggcacagtg
      661 ccgcccggcc tggacgagga gcccacgttc cgctgtgacg agtgtgacga actcttccag
      721 tccaagctgg acctgcggcg ccataagaag tacacgtgtg gctcagtggg ggctgcgctc
      781 tacgagggcc tggctgagga gctcaagccc gagggccttg gcggtggcag cggccaagcc
      841 cacgagtgca aggactgcga gcggatgttc cccaacaagt acagcctgga gcagcacatg
      901 gtcatccaca cggaggagcg cgagtacaaa tgcgaccagt gtcccaaggc cttcaactgg
      961 aagtccaacc tcatccgcca ccagatgtcc cacgacagcg gcaaacgctt cgaatgtgaa
     1021 aactgcgtga aggtgttcac ggaccccagc aaccttcagc ggcacatccg ctcgcagcac
     1081 gtgggcgctc gggcccacgc ctgccccgac tgcgggaaga ccttcgccac gtcctccggc
     1141 ctcaagcagc acaagcatat ccacagcacg gtgaagcctt tcatatgtga ggtctgccac
     1201 aagtcctaca cgcagttctc caacctgtgc cggcacaagc ggatgcacgc cgactgccgc
     1261 acgcagatca agtgcaagga ctgtggccag atgttcagca ctacctcctc cctcaacaag
     1321 caccggcgct tctgcgaggg caagaaccat tacacgccgg gcggcatctt tgccccgggc
     1381 ctgcccttga cccccagccc catgatggac aaggcaaaac cctcccccag cctcaatcac
     1441 gccagcctgg gcttcaacga gtactttccc tccaggccgc acccggggag cctgcccttc
     1501 tccacggcgc ctcccacgtt ccccgcactc acccccggct tcccgggcat cttccctcca
     1561 tccttgtacc cccggccgcc tctgctacct cccacatcgc tgctcaagag ccccctgaac
     1621 cacacccagg acgccaagct ccccagtccc ctggggaacc cagccctgcc cctggtctcc
     1681 gccgtcagca acagcagcca gggcacgacg gcagctgcgg ggcccgagga gaagttcgag
     1741 agccgcctgg aggactcctg tgtggagaag ctgaagacca ggagcagcga catgtcggac
     1801 ggcagtgact ttgaggacgt caacaccacc acggggaccg acctggacac gaccacgggg
     1861 acgggctcgg acctggacag cgacgtggac agcgaccctg acaaggacaa gggcaagggc
     1921 aagtccgccg agggccagcc caagtttggg ggcggcttgg cgcccccggg ggccccgaac
     1981 agcgtggccg aggtgcctgt cttctattcc cagcactcat tcttcccgcc acccgacgag
     2041 cagctgctga ctgcaacggg cgccgccggg gactccatca aggccatcgc atccattgcc
     2101 gagaagtact ttggccccgg cttcatgggg atgcaggaga agaagctggg ctcgctcccc
     2161 taccactcgg cgttcccctt ccagttcctg cccaacttcc cccactccct ttaccccttc
     2221 acggaccgag ccctcgccca caacttgctg gtcaaggccg agccaaagtc accccgggac
     2281 gccctcaagg tgggcggccc cagtgccgag tgcccctttg atctcaccac caagcccaaa
     2341 gacgtgaagc ccatcctgcc catgcccaag ggcccctcgg cccccgcatc cggcgaggag
     2401 cagccgctgg acctgagcat cggcagccgg gcccgtgcca gccaaaacgg cggcgggcgg
     2461 gagccccgca agaaccacgt ctatggggaa cgcaagctgg gcgccggcga ggggctgccc
     2521 caggtgtgcc cggcgcggat gccccagcag cccccgctcc actacgccaa gccctcgccc
     2581 ttcttcatgg accccatcta cagcagggta gaaaagcgga aggtcacaga ccccgtggga
     2641 gccctgaagg agaagtacct gcggccgtcc ccgctgctct tccaccccca gatgtcagcc
     2701 atagagacca tgacagagaa gctggagagc tttgcagcca tgaaggcgga ctcgggcagc
     2761 tccctgcagc ccctccccca ccaccccttc aacttccggt ccccaccccc aacgctctcc
     2821 gaccccatcc tcaggaaggg caaggagcga tacacgtgca ggtactgtgg gaagatcttc
     2881 cccagatcag ccaatctcac cagacacctg aggacgcaca ctggggagca gccgtacagg
     2941 tgtaagtact gcgaccgctc cttcagcatc tcttcgaacc tccagcggca cgtccggaac
     3001 atccacaaca aggagaagcc tttcaagtgc cacctgtgca accgctgctt cgggcagcag
     3061 accaacctgg accggcacct caagaagcac gagcacgaga acgcaccagt gagccagcac
     3121 cccggggtcc tcacgaacca cctggggacc agcgcgtcct ctcccacctc agagtcggac
     3181 aaccacgcac ttttagacga gaaagaagac tcttatttct cggaaatcag aaactttatt
     3241 gccaatagtg agatgaacca agcatcaacg cgaacagaga aacgggcgga catgcagatc
     3301 gtggacggca gtgcccagtg tccaggccta gccagtgaga agcaggagga cgtggaggag
     3361 gaggacgacg atgacctgga ggaggacgat gaggacagcc tggccgggaa gtcgcaggat
     3421 gacaccgtgt cccccgcacc cgagccccag gccgcctacg aggatgagga ggatgaggag
     3481 ccagccgcct ccctggccgt gggctttgac cacacccgaa ggtgtgctga ggaccacgaa
     3541 ggcggtctgt tagctttgga gccgatgccg acttttggga aggggctgga cctccgcaga
     3601 gcagctgagg aagcatttga agttaaagat gtgcttaatt ccaccttaga ttctgaggct
     3661 ttaaaacata cactgtgcag gcaggctaag aaccaggcat atgcaatgat gctgtccctt
     3721 tccgaagaca ctcctctcca caccccctcc cagggttctc tggacgcttg gttgaaggtc
     3781 actggagcca cgtcggagtc tggagcattt caccccatca accacctcta gtaatgaact
     3841 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     3901 gactaaatat gcttcccacg tgcacgcgtt aagtcgacaa tcaacctctg gattacaaaa
     3961 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4021 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4081 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4141 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4201 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4261 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4321 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     4381 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4441 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4501 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     4561 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     4621 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     4681 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     4741 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     4801 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat
     4861 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     4921 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4981 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5041 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5101 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5161 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     5221 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     5281 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5341 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5401 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5461 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5521 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5581 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5641 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5701 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5761 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5821 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     5881 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5941 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6001 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6061 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6121 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6181 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6241 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6301 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6361 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6421 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6481 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6541 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6601 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6661 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6721 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6781 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6841 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6901 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6961 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7021 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7081 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7141 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7201 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7261 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7321 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7381 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7441 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7501 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7561 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7621 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7681 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7741 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7801 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     7861 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     7921 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7981 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     8041 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     8101 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     8161 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     8221 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     8281 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     8341 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8401 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8461 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     8521 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     8581 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     8641 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     8701 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     8761 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     8821 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     8881 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     8941 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     9001 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     9061 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     9121 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     9181 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     9241 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9301 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9361 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9421 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9481 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9541 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9601 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9661 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     9721 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     9781 accccgaggg gacccatgca ttgcatctca attagtcagc aaccaggtgt ggaaagtccc
     9841 caggctcccc agcaggcaga agtatgcaaa gcatgcgtct caattagtca gcaaccatag
     9901 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     9961 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
    10021 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttcta
    10081 gaggtaccac cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
    10141 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
    10201 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
    10261 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg
    10321 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
    10381 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
    10441 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
    10501 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
    10561 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
    10621 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
    10681 acccgcaagc ccggtgccag ttaacgaatt ctagatcttg agacaaatgg cagtattcat
    10741 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
    10801 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
    10861 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg aggcctgcag
    10921 gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
    10981 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
    11041 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
    11101 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
    11161 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
    11221 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
    11281 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
    11341 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
    11401 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
    11461 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
    11521 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
    11581 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
    11641 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
    11701 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
    11761 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
    11821 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
    11881 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
    11941 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
    12001 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
    12061 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
    12121 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
    12181 ggctagcacc gccacc
//
