LOCUS       sequence-326359-       12349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2358
FEATURES             Location/Qualifiers
     source          1..12349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5287..5875
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5340..5360)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5758..5769)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5947..6180
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6258..6379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6295..6314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6349..6368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6419..6554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6574..6593)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6575..6593)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6603..6620)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6603..6619)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6612..6634)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6761..7216
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6848..6867)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7058..7079
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7242..7346
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7347..7415,7416..8207)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7565..7584)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8378..8966
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8867..8886
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9120..9137
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9254..9275
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9290..9295,9296..9313,9314..9320)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9328..9344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9333..9355
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9352..9368
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9352..9368
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9387..9407
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9389..9407
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9435..9661
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9662..9842
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9889..10014
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10507..10740
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10925..10969
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11200..11410
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11222..11418
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11269..11404
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11450..12052
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11450..11469)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11946..11966
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12095..12212
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgag
     1201 atcgttcttg cagcaagatg ttaataagac aaaatctaga ctaaatgtgt taaatgggct
     1261 tgccaacaat atggatgatt tgaagataaa caccgatatt actggtgcta aagaagaact
     1321 cctagatgac aacaatttta tctcagacaa agagagcgga gttcataagc caaaagattg
     1381 tcaaacatca tttcagaaaa ataatacgtt gactctgcct gaagaactgt caaaggacaa
     1441 atctgaaaac gccttaagtg gaggccagtc tagtctattt atacatgctg gtgctcctac
     1501 tgtttctagt gaaaacttta tcttgcctaa aggagctgct gttaatggac cagtttcaca
     1561 ctcctcctta actaagactt ccaatatgaa taaaggcagt gtttcattaa ccactggaca
     1621 gcctgtggat cagccaacaa cagaatcttg ttcaactttg aaggtagcag ctgatcttca
     1681 gctgtctaca ccacagaaag caagtcaaca ccaagtttta tttttgttat cagatgtagc
     1741 acatgctaag aatcccaccc attccaataa aaaactacct acctctgctt cagttggttg
     1801 tgacattcag aattcagtag ggagtaatat aaagtcagat ggcactttaa taaatcaagt
     1861 agaggtgggt gaggatggtg aagatttatt ggtgaaagat gattgtgtca atacagtaac
     1921 gggaatttcc tcaggtacag atggatttag gtcagaaaat gatacaaact gggatcccca
     1981 aaaagagttc attcaatttc ttatgactaa tgaggaaaca gtagataaag ctccacctca
     2041 ttctaaaata ggtctagaaa aaaaaagaaa gcgaaaaatg gatgtaagca agataactcg
     2101 ttataccgag gattgcttta gtgattctaa ttgtgtaccc aataaatcaa aaatgcaaga
     2161 agtagacttt ctagaacaaa atgaagagct acaagcagta gactcacaga aatatgcatt
     2221 atcaaaagtg aagcctgaat caactgatga agacttagaa tctgtggatg ccttccaaca
     2281 tctaatttat aacccagata agtgtggaga agagagttca cctgttcata ctagcacttt
     2341 tctttcaaat accttaaaaa agaaatgtga agagagtgat tctgagtcac ctgctacttt
     2401 cagtaccgaa gagccatcat tctacccctg tacaaagtgc aatgtgaatt ttagggagaa
     2461 gaagcacctc cacaggcata tgatgtatca tttagatggg aatagtcact ttcgccatct
     2521 taatgtccca aggccatatg cttgtagaga atgtggacgg acatttcgag atcgcaattc
     2581 acttctaaaa catatgatta ttcaccagga gagaagacag aagttgatgg aggaaattcg
     2641 tgaattgaaa gaacttcagg atgaaggaag aagtgcacga ttacagtgtc ctcagtgtgt
     2701 gtttggtacc aattgcccta aaacatttgt gcaacatgct aaaacccatg aaaaagataa
     2761 aaggtactac tgctgtgaag agtgtaactt catggcagtg acagaaaatg aattggaatg
     2821 ccatcgaggc attgcacatg gggcagtggt aaaatgccct atggtcactt ctgatattgc
     2881 ccagagaaaa acacaaaaaa agactttcat gaaagactct gtagtaggat catccaaaaa
     2941 atcagctacc tacatatgta agatgtgtcc ttttactact tcagccaaaa gtgttttaaa
     3001 aaagcacacg gagtacttgc attcatcatc atgtgttgat tcatttggta gtcctcttgg
     3061 acttgataaa agaaaaaatg acatccttga agaacctgta gatagtgata gcactaaaac
     3121 attaactaaa caacagtcaa ccacatttcc aaagaactct gctttaaaac aagatgtgaa
     3181 gcgaacattt ggatcaacct cacaatcaag tagtttttca aaaattcata agcggccaca
     3241 cagaatacag aaagctcgga aaagcattgc ccaatcaggt gtaaacatgt gcaatcaaaa
     3301 cagctctcct cataagaatg ttacaattaa aagcagcgtt gaccaaaaac ctaagtattt
     3361 ccatcaagca gcaaaagaaa agtctaatgc caaggcaaat agccactatt tgtatagaca
     3421 caaatatgaa aactatagga tgatcaaaaa atcaggtgaa tcatatcctg tgcatttcaa
     3481 aaaagaagaa gctagttcat taaattcttt acacctgttt tcatcatcaa gtaattctca
     3541 caacaatttt atttcagacc ctcataagcc tgacgccaaa aggcctgaaa gcttcaaaga
     3601 tcacagacgt gtagctgtaa agagagtaat taaggaatct aagaaggaaa gttctgttgg
     3661 aggggaagac ttggatagct atccagattt tttgcataaa atgactgttg tcgttttgca
     3721 aaaacttaat tctgctgaaa agaaagatag ttatgaaaca gaagatgaaa gttcctggga
     3781 taatgttgag ttaggagact acactacaca ggccatagaa gatgaaacct atagtgatat
     3841 taatcaagag catgtaaatt tattcccttt atttaagagc aaagtggaag gtcaggagcc
     3901 tggagaaaat gctactctta gttatgacca aaacgatggc ttttattttg aatactatga
     3961 agatactgga agtaacaact ttttgcatga gatacatgat cctcagcatt tagaaactgc
     4021 agatgcttca ttgtcaaagc atagttctgt ttttcattgg actgatttgt ctcttgagaa
     4081 gaaatcgtgt ccttactgcc cagcaacatt tgaaacaggt gttgggttat caaatcatgt
     4141 cagggggcat cttcacagag caggattaag ctatgaagcc cgtcatgttg tatcaccaga
     4201 acaaatagcc acaagtgaca aaatgcagca tttcaaaaga actggcacag gaacacctgt
     4261 taaacgagtt agaaaagcta tagagaagtc tgaaaccact tctgaacaca cttgtcagct
     4321 ctgtggtggt tggtttgata ctaaaattgg attatcaaat catgttagag gccacttgaa
     4381 aagacttgga aagacgaaat gggatgctca caaatctcca atctgtgttc tgaatgagat
     4441 gatgcaaaat gaagaaaaat atgaaaaaat cttaaaggca ttgaacagtc gtcgtattat
     4501 tcccagacca tttgtagctc aaaaacttgc atcaagtgat gactttatat ctcaaaatgt
     4561 tatacctctt gaagcatacc gtaatggcct aaagactgaa gctctgtcag tgtctgcatc
     4621 agaagaagaa gggctgaatt tcttaaatga atatgatgaa acaaaaccag aactgcccag
     4681 tgggaaaaag aatcagtctc ttacactcat agaacttctt aaaaataaaa ggatgggaga
     4741 agaaaggaat tctgctattt ctcctcaaaa gatccataat cagacagcaa gaaagagatt
     4801 cgttcagaaa tgcgttcttc cattaaatga ggatagtccg ttgatgtatc agccacaaaa
     4861 aatggacttg actatgcact cagccttaga ttgtaagcaa aagaaatcaa ggtcaagatc
     4921 tggaagcaag aagaaaatgc taacattacc tcatggtgct gacgaggttt acattctccg
     4981 atgcaggttt tgtggcctag tctttcgagg acccttgtct gttcaggaag actggattaa
     5041 gcacttacaa cgacatattg taaacgctaa tcttccacgg actggagctg gcatggtgga
     5101 agtcacgtca ctacttaaaa agcctgcctc cattacagaa acttcatttt ctctactaat
     5161 ggccgaagca gcttcatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     5221 gctttatata tcttgtggaa aggacgagat cgaacacaga cgtccacgct tacgcgttaa
     5281 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5341 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5401 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5461 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5521 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5581 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5641 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     5701 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5761 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5821 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     5881 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5941 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     6001 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6061 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6121 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6181 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6241 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6301 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6361 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     6421 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6481 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6541 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     6601 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     6661 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6721 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     6781 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6841 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6901 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6961 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7021 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7081 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7141 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     7201 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7261 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7321 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7381 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7441 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7501 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7561 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7621 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7681 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7741 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7801 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7861 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7921 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7981 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8041 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8101 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8161 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8221 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8281 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8341 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8401 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8461 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8521 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8581 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8641 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8701 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8761 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8821 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8881 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8941 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9001 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9061 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9121 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9181 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9241 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9301 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9361 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     9421 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     9481 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     9541 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     9601 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     9661 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     9721 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     9781 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     9841 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     9901 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     9961 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    10021 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    10081 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    10141 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    10201 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    10261 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    10321 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
    10381 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
    10441 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
    10501 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
    10561 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
    10621 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
    10681 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
    10741 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
    10801 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
    10861 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
    10921 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
    10981 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
    11041 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
    11101 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
    11161 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    11221 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    11281 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    11341 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    11401 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
    11461 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
    11521 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
    11581 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
    11641 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
    11701 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
    11761 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
    11821 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
    11881 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
    11941 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
    12001 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
    12061 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    12121 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    12181 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    12241 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
    12301 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
