LOCUS       sequence-326374-        8317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP-expression vector for human H11 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Pryzhkova MV, Jordan PW
  TITLE     Adaptation of Human Testicular Niche Cells for Pluripotent Stem Cell
            and Testis Development Research.
  JOURNAL   Tissue Eng Regen Med. 2020 Apr;17(2):223-235. doi: 
            10.1007/s13770-020-00240-0. Epub 2020 Feb 29.
  PUBMED    32114677
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s13770-020-00240-0"; journalName: "Tissue Eng Regen Med"; 
            date: "2020-04"; volume: "17"; issue: "2"; pages: "223-235"
COMMENT     Sequence Label: p274 EF1a_EGFP_hH11
FEATURES             Location/Qualifiers
     source          1..8317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(35..41,42..59,60..65))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    80..101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(218..235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(389..977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(469..488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1148..1939,1940..2008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1771..1790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2009..2113)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2140..2595
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2227..2246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2437..2458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2721..2743
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2735..2752
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2736..2752
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3568..4746
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3799..4737
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4694..4714
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4760..4769
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4766..4768,4769..4771,4772..5485)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4766..4768,4769..4771,4772..5482)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4811..4832)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5072..5091)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5419..5440
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5572..5591)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5637..5692
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5691..5710)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    complement(5752..5785)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5822..6321
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5857..5880)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6254..6273
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6361..6960
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6361..6380)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6857..6877
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(7541..7574)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
       61 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      121 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      181 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      241 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1141 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1201 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1261 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1321 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1381 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1441 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1501 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1561 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1621 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1681 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1741 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     1801 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     1861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     1921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     1981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2101 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     2161 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2221 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2281 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2341 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2401 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2461 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2521 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     2581 taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     2641 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     2701 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
     2761 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
     2821 aatcaatatt ggctattggc cattgcatac gtttcttgtg aactcatctc gtgcctgcaa
     2881 gacatcaaaa gtttctccaa agacatccct tcctctctgc cctcatccta tatcaaggtc
     2941 ttagctcctt gaagactgca ttaatgtgtc ttttgccttc atcccccccc caccctcccg
     3001 cttcctctag tccttgtgtt taccaaaata cttttgctaa aacctgtatg tctagcttct
     3061 gctgatactc ttagcaatac tcccttattt ccttctggcg ccatttgcca atcaccagct
     3121 aatggctttg ctctttgcat ggtacctgtt tctgtcctac tagcttctag tctgaaaagg
     3181 aagcctttac tagcagcttc aacccagtcc tccttacctt gcacctatta acgttggttc
     3241 aacatgaagg ggaagtaaga taagtggaag aggatacaca ggaaaggtgg aactggattc
     3301 aggtatctgt gttctgccat tgacacttgg gactgcgttc cagcttccac ctgtggaaac
     3361 cagaaagcat ttgccaaaaa ttctaaacag agggtctatt tctgaagctg aggaatcaca
     3421 tggagtgaat agcatggggg aaggggatta ctaatgaggc tgaaagaaaa gtaccaggaa
     3481 gagactgact tcatgcataa tccataaagc caggttttct tgaaaggtga cctgctttct
     3541 tgtattcctg caagaaacgg ctcgaggggc tccggtgccc gtcagtgggc agagcgcaca
     3601 tcgcccacag tccccgagaa gttgtgggga ggggtcggca attgaagcgg tgcctagaga
     3661 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     3721 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     3781 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
     3841 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
     3901 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     3961 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     4021 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     4081 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     4141 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     4201 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     4261 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     4321 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     4381 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     4441 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     4501 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     4561 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     4621 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     4681 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     4741 tcgtgagggg atcgtcgacg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     4801 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     4861 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     4921 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     4981 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     5041 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     5101 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     5161 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     5221 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     5281 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     5341 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     5401 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     5461 cggcatggac gagctgtaca agtaaagcgg ccgcactcct caggtgcagg ctgcctatca
     5521 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     5581 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     5641 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     5701 gacatatggg agggcgatcg ccagtactag tgaacctctt cgagggacct aataacttcg
     5761 tatagcatac attatacgaa gttatattaa gggttccgga tctcgacctc gaaattctac
     5821 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     5881 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     5941 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     6001 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     6061 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     6121 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     6181 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     6241 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     6301 tcctcatctc cgggcctttc gacctgcatc catctagatc tcgagcagct gaagcttacc
     6361 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6421 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6481 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6541 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6601 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6661 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6721 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6781 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6841 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6901 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6961 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgatg
     7021 atatcagatc cccgggatgc agaaattgat gatctattaa acaataaaga tgtccactaa
     7081 aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta cctacatttt
     7141 gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa atgcctgctc
     7201 tttactgaag gctctttact attgctttat gataatgttt catagttgga tatcataatt
     7261 taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat ctatagatct
     7321 atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt tctaagtact
     7381 gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc ctctgttcca
     7441 catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa gctggtcgag
     7501 atcctaagct tggctggacg taaactcctc ttcagaccta ataacttcgt atagcataca
     7561 ttatacgaag ttatattaag ggttattgaa tatgatcgga attgcggccc tgacctgttg
     7621 gggtctttaa agctcaagga aaaaggccat agttgatttc tcctaaatca agatagagtc
     7681 caattaactt tttttttttt ttaagatgga gtctcactct gtcgccaggc tggactgcag
     7741 tggcgtgatc tcagctcact gcaacctctg cctcccaggt tcaggcgatt cttctgtctc
     7801 agcctcccga gtagctggga ctacaagtgt gggccaccat gcccagctag ttttgtattt
     7861 ttagtagaga tgggatttca ccatcttggc taggatggtc ttgatctctt gaccttgtga
     7921 ttcgcctgcc tctgcctccc agagtgctga gattacaggc gtgagccaca gtgcccaatt
     7981 tccatgggtt ttcaagaaaa acttaactta cttaaacttc agatcacttc cttaaaggaa
     8041 catactgagc atagcttggc tggttagaaa ttaggaagac tagcttgggc tacatggtaa
     8101 gaccctgcct ctacaaaaaa taagaaaaaa gttagtggca tgtgcctgta gttccatcta
     8161 cttgggaggc tgaggtgaga ggatcgcttg agcccaggag gttgaagctg cagtgagcca
     8221 cgattgcacc actgtactcc agcctgggtg acacagagtg agaccctgtc tccaaacaaa
     8281 attaggaagg gtttcagaga ggaaataaac acggccg
//
