LOCUS       sequence-326382-       10624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1644
FEATURES             Location/Qualifiers
     source          1..10624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3562..4150
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3615..3635)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4033..4044)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4222..4455
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4533..4654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4570..4589)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4624..4643
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4694..4829
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4849..4868)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4850..4868)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4878..4895)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4878..4894)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4887..4909)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5036..5491
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5123..5142)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5333..5354
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5517..5621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5622..5690,5691..6482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5840..5859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6653..7241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7142..7161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7395..7412
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7529..7550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7565..7570,7571..7588,7589..7595)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7603..7619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7608..7630
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7627..7643
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7627..7643
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7662..7682
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7664..7682
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7710..7936
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7937..8117
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8164..8289
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8782..9015
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9200..9244
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9475..9685
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9497..9693
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9544..9679
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9725..10327
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9725..9744)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10221..10241
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10370..10487
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 cgagccggct tcggtaactg tgacatttga tgatgtggcc ttatattttt cggaacaaga
     1261 gtgggagatc ctggagaagt ggcagaagca aatgtataag caagagatga agaccaatta
     1321 cgagaccctt gattccctgg ggtatgcttt ttccaagcca gatttgatca catggatgga
     1381 acaagggaga atgctattaa tcagcgaaca gggatgctta gataaaacaa gaaggacaac
     1441 tagccctcct actgatgaac agttgaacat gaagaatact ggaaagttgc tatgttttga
     1501 tgacgaagga actcccagga caaaagaaga ggattgccgt ttaaatggtc ctcaaaaaca
     1561 ggacttgtgt gctgccttac gagggaaaga gagaaagatt ttattagctc aaacagccac
     1621 cttccagtct ccaagtctcc gagaaacaga gattctaaat aaaaaagtca gcatcacagc
     1681 atatgatcca gacaagaaag acctgcggca taagcctcgg gagaccccag ggcgcttaga
     1741 aattcccaca gggccaagat gctattcctg ctatgtctgt aggaaagtct tccaagtaag
     1801 gagggatttg ctaaagcaca aacggagcca ctccaagagc cagctctgca gatacccaaa
     1861 gtacaaaaac tcgtccagag ggaagtccga actcaggcgg acgcagaggc tcctgtgtca
     1921 gaagaagcgg ttccagtgca gtgagtgtga gaagagctac ttcctgaagg gcagcctcgt
     1981 cactcatcag gttgtccaca ccggccagcg gccctaccca tgccctgagt gcgacaagac
     2041 cttccggtac agggccaacc tgaagaagca cctgtgtcta caccgcgggg agcggccgtt
     2101 ctgctgcggg gagtgcggcc gggccttcgt gcagcagtgc gagctcacgg agcacttgcg
     2161 gctgcacagc ggagagaagc ccttccagtg tccgcagtgt gaccggtgct tccgcctgaa
     2221 gaggggcatg aaggtccatc tgacccagca cagcgggaag aggcccttcc actgtcccga
     2281 gtgtggccgg agcttctccc ggaaggctgc cctgaagacc caccagagga cgcacagcga
     2341 ggaaaagccg ttttcttgtg gtgaatgtgg caggaaattc atctacaaga ttaagctgga
     2401 cgagcacatc agagttcaca cgggagagaa gcccttttcg tgtcccgagt gtaacaaaag
     2461 tttccgcctc aagagaagcc tgaaagccca cgggctgcag cacattggga agcggccctt
     2521 ccagtgcccg gagtgcagca ggggcttctt ctggaggaac gccatgcgcg cccaccagcg
     2581 cctgcacagc gagcaaaagc ccttcccctg cgccgagtgc ggcaagcgct tcacgcggcc
     2641 ttccaagctc gcctgccaca ccagagtcca cgacaggcag aaggagtttc cctgcggcga
     2701 gtgcaaaaag accttttctc agcagtcgcg gctcacgcag cacctgaagg tccacaccac
     2761 ggaaaagccg ttctcctgcg ccgagtgcgg ccgcagtttc cgccgacgcg cgcatctcac
     2821 agagcacacg aggcttcaca gtggcgagga gcccttccag tgtcccgagt gcgacaagag
     2881 cttctcctgg aaggcctcca tgaagttcca ccagcggatg cacagggacg agaagccctt
     2941 cgcgtgcggt gagtgtgaca agacctacac gcatcagtct cagctcaccg agcacctgag
     3001 gctgcacagt ggagagaagc cctaccagtg tcctgaatgc gagaagactt tccgcctcaa
     3061 gggaaacctg aaaagccacc tgctgcagca cagtggccaa aagccattct cttgtgtgat
     3121 gtgcggcaaa agtttcactc aacagtaccg gctcacagaa cacattcgag tccacagcgg
     3181 ggagaagccc ttccagtgtc cggagtgtga caagagctac tgcatcaggg gcagcttgaa
     3241 ggtccacttg tataagcaca gtggagagag gcccttccag tgtcccgagt gtggcaaagg
     3301 cttcctccag aagagaagcc tgaaggccca tctgtgcctt cacagtgggg agaggccttt
     3361 ttcttgtgat gagtgtggaa ggagcttcac gtacgtgggg gcgctcaaga cccacattgc
     3421 agtgcatgcc aaagagaagc cctccagcct ctagtaatga actagtccgt aacttgaaag
     3481 tatttcgatt tcttggcttt atatatcttg tggaaaggac gatggatcat ttcgcaacta
     3541 tgttaaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     3601 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3661 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3721 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3781 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3841 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3901 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     3961 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4021 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4081 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4141 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     4201 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     4261 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4321 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4381 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4441 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     4501 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     4561 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4621 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     4681 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4741 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4801 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     4861 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4921 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4981 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     5041 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5101 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5161 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5221 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5281 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5341 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5401 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5461 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     5521 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5581 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5641 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5701 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5761 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5821 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5881 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5941 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6001 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6061 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6121 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6181 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6241 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6301 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6361 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6421 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6481 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6541 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6601 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6661 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6721 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6781 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6841 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6901 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6961 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7021 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7081 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7141 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7201 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7261 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7321 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7381 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7441 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     7501 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7561 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7621 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     7681 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     7741 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     7801 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     7861 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     7921 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7981 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8041 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8101 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     8161 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     8221 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8281 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8341 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8401 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8461 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     8521 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8581 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     8641 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8701 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8761 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8821 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8881 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8941 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     9001 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     9061 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     9121 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     9181 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9241 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     9301 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9361 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     9421 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9481 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9541 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9601 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9661 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     9721 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     9781 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     9841 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     9901 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     9961 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
    10021 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
    10081 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
    10141 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
    10201 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
    10261 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
    10321 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
    10381 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
    10441 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
    10501 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
    10561 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
    10621 aatt
//
