LOCUS       sequence-326383-       10735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10735)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2032
FEATURES             Location/Qualifiers
     source          1..10735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3673..4261
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3726..3746)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4144..4155)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4333..4566
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4644..4765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4681..4700)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4735..4754
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4805..4940
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4960..4979)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4961..4979)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4989..5006)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4989..5005)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4998..5020)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5147..5602
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5234..5253)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5444..5465
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5628..5732
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5733..5801,5802..6593)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5951..5970)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6764..7352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7253..7272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7506..7523
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7640..7661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7676..7681,7682..7699,7700..7706)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7714..7730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7719..7741
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7738..7754
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7738..7754
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7773..7793
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7775..7793
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7821..8047
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8048..8228
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8275..8400
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8893..9126
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9311..9355
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9586..9796
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9608..9804
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9655..9790
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9836..10438
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9836..9855)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10332..10352
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10481..10598
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 aatggacatg gaggatgcgg atatgactct gtggacagag gctgagtttg aagagaagtg
     1261 tacatacatt gtgaacgacc acccctggga ttctggtgct gatggcggta cttcggttca
     1321 ggcggaggca tccttaccaa ggaatctgct tttcaagtat gccaccaaca gtgaagaggt
     1381 tattggagtg atgagtaaag aatacatacc aaagggcaca cgttttggac ccctaatagg
     1441 tgaaatctac accaatgaca cagttcctaa gaacgccaac aggaaatatt tttggaggat
     1501 ctattccaga ggggagcttc accacttcat tgacggcttt aatgaagaga aaagcaactg
     1561 gatgcgctat gtgaatccag cacactctcc ccgggagcaa aacctggctg cgtgtcagaa
     1621 cgggatgaac atctacttct acaccattaa gcccatccct gccaaccagg aacttcttgt
     1681 gtggtattgt cgggactttg cagaaaggct tcactaccct tatcccggag agctgacaat
     1741 gatgaatctc acacaaacac agagcagtct aaagcaaccg agcactgaga aaaatgaact
     1801 ctgcccaaag aatgtcccaa agagagagta cagcgtgaaa gaaatcctaa aattggactc
     1861 caacccctcc aaaggaaagg acctctaccg ttctaacatt tcacccctca catcagaaaa
     1921 ggacctcgat gactttagaa gacgtgggag ccccgaaatg cccttctacc ctcgggtcgt
     1981 ttaccccatc cgggcccctc tgccagaaga ctttttgaaa gcttccctgg cctacgggat
     2041 cgagagaccc acgtacatca ctcgctcccc cattccatcc tccaccactc caagcccctc
     2101 tgcaagaagc agccccgacc aaagcctcaa gagctccagc cctcacagca gccctgggaa
     2161 tacggtgtcc cctgtgggcc ccggctctca agagcaccgg gactcctacg cttacttgaa
     2221 cgcgtcctac ggcacggaag gtttgggctc ctaccctggc tacgcacccc tgccccacct
     2281 cccgccagct ttcatcccct cgtacaacgc tcactacccc aagttcctct tgccccccta
     2341 cggcatgaat tgtaatggcc tgagcgctgt gagcagcatg aatggcatca acaactttgg
     2401 cctcttcccg aggctgtgcc ctgtctacag caatctcctc ggtgggggca gcctgcccca
     2461 ccccatgctc aaccccactt ctctcccgag ctcgctgccc tcagatggag cccggaggtt
     2521 gctccagccg gagcatccca gggaggtgct tgtcccggcg ccccacagtg ccttctcctt
     2581 taccggggcc gccgccagca tgaaggacaa ggcctgtagc cccacaagcg ggtctcccac
     2641 ggcgggaaca gccgccacgg cagaacatgt ggtgcagccc aaagctacct cagcagcgat
     2701 ggcagccccc agcagcgacg aagccatgaa tctcattaaa aacaaaagaa acatgaccgg
     2761 ctacaagacc cttccctacc cgctgaagaa gcagaacggc aagatcaagt acgaatgcaa
     2821 cgtttgcgcc aagactttcg gccagctctc caatctgaag gtccacctga gagtgcacag
     2881 tggagaacgg cctttcaaat gtcagacttg caacaagggc tttactcagc tcgcccacct
     2941 gcagaaacac tacctggtac acacgggaga aaagccacat gaatgccagg tctgccacaa
     3001 gagatttagc agcaccagca atctcaagac ccacctgcga ctccattctg gagagaaacc
     3061 ataccaatgc aaggtgtgcc ctgccaagtt cacccagttt gtgcacctga aactgcacaa
     3121 gcgtctgcac acccgggagc ggccccacaa gtgctcccag tgccacaaga actacatcca
     3181 tctctgtagc ctcaaggttc acctgaaagg gaactgcgct gcggccccgg cgcctgggct
     3241 gcccttggaa gatctgaccc gaatcaatga agaaatcgag aagtttgaca tcagtgacaa
     3301 tgctgaccgg ctcgaggacg tggaggatga catcagtgtg atctctgtag tggagaagga
     3361 aattctggcc gtggtcagaa aagagaaaga agaaactggc ctgaaagtgt ctttgcaaag
     3421 aaacatgggg aatggactcc tctcctcagg gtgcagcctt tatgagtcat cagatctacc
     3481 cctcatgaag ttgcctccca gcaacccact acctctggta cctgtaaagg tcaaacaaga
     3541 aacagttgaa ccaatggatc cttagtaatg aactagtccg taacttgaaa gtatttcgat
     3601 ttcttggctt tatatatctt gtggaaagga cgaccaggaa cctgtcaggc taattcgacg
     3661 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3721 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3781 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3841 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     3901 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     3961 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4021 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     4081 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4141 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4201 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4261 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4321 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     4381 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4441 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4501 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4561 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     4621 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     4681 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4741 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     4801 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4861 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4921 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     4981 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     5041 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     5101 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     5161 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5221 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5281 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5341 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5401 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5461 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5521 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5581 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5641 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5701 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5761 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5821 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5881 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5941 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6001 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6061 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6121 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6181 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6241 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6301 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6361 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6421 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6481 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6541 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6601 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6661 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6721 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6781 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6841 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6901 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6961 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7021 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7081 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7141 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7201 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7261 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7321 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7381 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7441 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7501 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7561 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     7621 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     7681 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     7741 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     7801 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     7861 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     7921 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     7981 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     8041 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8101 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8161 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8221 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     8281 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     8341 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     8401 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     8461 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     8521 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     8581 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     8641 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     8701 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     8761 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     8821 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     8881 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     8941 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     9001 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     9061 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     9121 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     9181 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     9241 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     9301 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     9361 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     9421 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     9481 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat
     9541 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9601 caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9661 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9721 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9781 tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac
     9841 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     9901 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     9961 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
    10021 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
    10081 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
    10141 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
    10201 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
    10261 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
    10321 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
    10381 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg
    10441 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    10501 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    10561 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    10621 gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac
    10681 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaatt
//
