LOCUS       sequence-32639-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   O'Connell RM, Chaudhuri AA, Rao DS, Baltimore D
  TITLE     Inositol phosphatase SHIP1 is a primary target of miR-155.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Apr 28. 106(17):7113-8.
  PUBMED    19359473
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Apr 28. 106(17):7113-8."
COMMENT     Sequence Label: MGP M155
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           90..116
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           180..221
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     promoter        279..778
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(314..337)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     711..730
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(799..818)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tcggcatgga cgagctgtac agtaaagcgg ccgcaaacca ggaaggggaa gtgtgtggtt
       61 taagttgcat atcccttatc ctctggctgc tggaggcttg ctgaaggctg tatgctgtta
      121 atgctaattg tgataggggt tttggcctct gactgactcc tacctgttag cattaacagg
      181 acacaaggcc tgttactagc actcacatgg aacaaatggc caccgtggga ggatgacaag
      241 tccaagagtc accctgctgg atctcgagga attctaccgg gtaggggagg cgcttttccc
      301 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
      361 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
      421 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
      481 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
      541 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
      601 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
      661 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
      721 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
      781 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
      841 gacgtcccc
//
