LOCUS       sequence-326397-        9372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive TIR1 expression vector for human H11 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Pryzhkova MV, Xu MJ, Jordan PW
  TITLE     Adaptation of the AID system for stem cell and transgenic mouse 
            research.
  JOURNAL   Stem Cell Res. 2020 Nov 5;49:102078. doi: 10.1016/j.scr.2020.102078.
  PUBMED    33202307
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2020-11-5"; volume: "49:102078. doi"; pages: " 
            10.1016/j.scr.2020.102078"
COMMENT     Sequence Label: p286 TIR1_HA_hH11
FEATURES             Location/Qualifiers
     source          1..9372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(35..41,42..59,60..65))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    80..101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(218..235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(389..977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(469..488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1148..1939,1940..2008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1771..1790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2009..2113)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2140..2595
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2227..2246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2437..2458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2721..2743
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2735..2752
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2736..2752
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3568..4746
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3799..4737
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4694..4714
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4766..6490
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             6500..6526
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6646..6767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6683..6702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6737..6756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(6807..6840)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6877..7376
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6912..6935)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7309..7328
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7416..8015
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7416..7435)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7912..7932
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(8596..8629)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
       61 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      121 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      181 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      241 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1141 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1201 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1261 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1321 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1381 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1441 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1501 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1561 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1621 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1681 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1741 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     1801 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     1861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     1921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     1981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2101 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     2161 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2221 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2281 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2341 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2401 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2461 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2521 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     2581 taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     2641 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     2701 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
     2761 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
     2821 aatcaatatt ggctattggc cattgcatac gtttcttgtg aactcatctc gtgcctgcaa
     2881 gacatcaaaa gtttctccaa agacatccct tcctctctgc cctcatccta tatcaaggtc
     2941 ttagctcctt gaagactgca ttaatgtgtc ttttgccttc atcccccccc caccctcccg
     3001 cttcctctag tccttgtgtt taccaaaata cttttgctaa aacctgtatg tctagcttct
     3061 gctgatactc ttagcaatac tcccttattt ccttctggcg ccatttgcca atcaccagct
     3121 aatggctttg ctctttgcat ggtacctgtt tctgtcctac tagcttctag tctgaaaagg
     3181 aagcctttac tagcagcttc aacccagtcc tccttacctt gcacctatta acgttggttc
     3241 aacatgaagg ggaagtaaga taagtggaag aggatacaca ggaaaggtgg aactggattc
     3301 aggtatctgt gttctgccat tgacacttgg gactgcgttc cagcttccac ctgtggaaac
     3361 cagaaagcat ttgccaaaaa ttctaaacag agggtctatt tctgaagctg aggaatcaca
     3421 tggagtgaat agcatggggg aaggggatta ctaatgaggc tgaaagaaaa gtaccaggaa
     3481 gagactgact tcatgcataa tccataaagc caggttttct tgaaaggtga cctgctttct
     3541 tgtattcctg caagaaacgg ctcgaggggc tccggtgccc gtcagtgggc agagcgcaca
     3601 tcgcccacag tccccgagaa gttgtgggga ggggtcggca attgaagcgg tgcctagaga
     3661 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     3721 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     3781 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
     3841 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
     3901 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     3961 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     4021 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     4081 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     4141 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     4201 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     4261 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     4321 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     4381 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     4441 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     4501 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     4561 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     4621 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     4681 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     4741 tcgtgagggg atcgtcgacg ccaccatgac ctacttcccc gaggaagtgg tggaacacat
     4801 cttcagcttt ctgcccgccc agagggacag aaacaccgtg tccctcgtgt gcaaagtgtg
     4861 gtacgagatc gagagactga gcagacgggg cgtgttcgtg ggcaactgtt acgctgtgcg
     4921 ggctggcaga gtggccgcca gatttcctaa tgtgcgggcc ctgaccgtga agggcaagcc
     4981 tcacttcgcc gacttcaacc tggtgccccc tgattggggc ggctacgctg gaccttggat
     5041 cgaggctgct gccagaggct gtcacggcct ggaagaactg agaatgaaga gaatggtggt
     5101 gtccgacgag agcctggaac tgctggccag aagcttcccc agattcagag ccctggtgct
     5161 gatctcctgc gagggcttct ctacagatgg cctggccgcc gtggccagcc attgcaagct
     5221 gctgagagag ctggatctcc aggaaaacga ggtggaagat agaggcccca gatggctgag
     5281 ctgcttcccc gacagctgta ccagcctggt gtccctgaac ttcgcctgca tcaaggggga
     5341 agtgaacgcc ggcagcctgg aaagactggt gtccagaagc cccaacctga gaagcctgag
     5401 actgaacaga tccgtgtccg tggacaccct ggccaagatc ctgctgagaa cccctaacct
     5461 ggaagatctg ggcaccggca acctgaccga cgacttccag accgagagct acttcaagct
     5521 gaccagcgcc ctggaaaagt gcaagatgct gcggagcctg agcggcttct gggacgcctc
     5581 tcctgtgtgc ctgagcttca tctaccccct gtgcgcccag ctgaccggcc tgaacctgtc
     5641 ttacgcccct acactggacg ccagcgacct gaccaagatg atcagcagat gcgtgaagct
     5701 ccagagactg tgggtgctgg actgcatcag cgacaaggga ctccaggtgg tggccagctc
     5761 ctgcaaggat ctccaggaac tgagagtgtt ccccagcgac ttctacgtgg ccggctacag
     5821 cgccgtgacc gaagagggac tggtggctgt gtctctgggc tgccccaagc tgaacagcct
     5881 gctgtacttc tgccaccaga tgaccaacgc cgccctcgtg accgtggcca agaactgccc
     5941 caacttcacc aggttcagac tgtgcatcct ggaacccggc aagcccgacg ttgtgaccag
     6001 ccagccactg gatgagggct tcggagccat cgtgcgcgag tgcaagggac tccagagact
     6061 gagcatcagc ggcctgctga ccgacaaggt gttcatgtac atcgggaagt acgccaagca
     6121 gctggaaatg ctgtctatcg ccttcgctgg cgactccgac aagggcatga tgcacgtgat
     6181 gaacggctgc aagaacctgc ggaaactgga aatcagagac agccccttcg gcgacgctgc
     6241 cctgctgggc aacttcgcca gatacgagac aatgagatcc ctgtggatga gcagctgcaa
     6301 cgtgacactg aagggctgcc aggtgctggc cagcaagatg cccatgctga acgtggaagt
     6361 gatcaacgag agggacggca gcaatgagat ggaagagaac cacggcgacc tgcctaaggt
     6421 ggaaaagctg tacgtgtaca gaaccacagc cggcgctagg gacgacgccc ctaacttcgt
     6481 gaagatcctg ggcggatctt acccatacga cgtaccagat tacgcttagg cgatcgcctg
     6541 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     6601 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     6661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtcgccagt
     6781 actagtgaac ctcttcgagg gacctaataa cttcgtatag catacattat acgaagttat
     6841 attaagggtt ccggatctcg acctcgaaat tctaccgggt aggggaggcg cttttcccaa
     6901 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     6961 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     7021 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     7081 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     7141 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     7201 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     7261 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     7321 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     7381 gcatccatct agatctcgag cagctgaagc ttaccatgac cgagtacaag cccacggtgc
     7441 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     7501 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     7561 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     7621 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     7681 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     7741 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     7801 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     7861 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     7921 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     7981 gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
     8041 cgaccgaaag gagcgcacga ccccatgcat cgatgatatc agatccccgg gatgcagaaa
     8101 ttgatgatct attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt
     8161 tgttaagaag ggtgagaaca gagtacctac attttgaatg gaaggattgg agctacgggg
     8221 gtgggggtgg ggtgggatta gataaatgcc tgctctttac tgaaggctct ttactattgc
     8281 tttatgataa tgtttcatag ttggatatca taatttaaac aagcaaaacc aaattaaggg
     8341 ccagctcatt cctcccactc atgatctata gatctataga tctctcgtgg gatcattgtt
     8401 tttctcttga ttcccacttt gtggttctaa gtactgtggt ttccaaatgt gtcagtttca
     8461 tagcctgaag aacgagatca gcagcctctg ttccacatac acttcattct cagtattgtt
     8521 ttgccaagtt ctaattccat cagaagctgg tcgagatcct aagcttggct ggacgtaaac
     8581 tcctcttcag acctaataac ttcgtatagc atacattata cgaagttata ttaagggtta
     8641 ttgaatatga tcggaattgc ggccctgacc tgttggggtc tttaaagctc aaggaaaaag
     8701 gccatagttg atttctccta aatcaagata gagtccaatt aacttttttt tttttttaag
     8761 atggagtctc actctgtcgc caggctggac tgcagtggcg tgatctcagc tcactgcaac
     8821 ctctgcctcc caggttcagg cgattcttct gtctcagcct cccgagtagc tgggactaca
     8881 agtgtgggcc accatgccca gctagttttg tatttttagt agagatggga tttcaccatc
     8941 ttggctagga tggtcttgat ctcttgacct tgtgattcgc ctgcctctgc ctcccagagt
     9001 gctgagatta caggcgtgag ccacagtgcc caatttccat gggttttcaa gaaaaactta
     9061 acttacttaa acttcagatc acttccttaa aggaacatac tgagcatagc ttggctggtt
     9121 agaaattagg aagactagct tgggctacat ggtaagaccc tgcctctaca aaaaataaga
     9181 aaaaagttag tggcatgtgc ctgtagttcc atctacttgg gaggctgagg tgagaggatc
     9241 gcttgagccc aggaggttga agctgcagtg agccacgatt gcaccactgt actccagcct
     9301 gggtgacaca gagtgagacc ctgtctccaa acaaaattag gaagggtttc agagaggaaa
     9361 taaacacggc cg
//
