LOCUS       sequence-326398-        8935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA vector for targeting mouse H11 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Pryzhkova MV, Xu MJ, Jordan PW
  TITLE     Adaptation of the AID system for stem cell and transgenic mouse 
            research.
  JOURNAL   Stem Cell Res. 2020 Nov 5;49:102078. doi: 10.1016/j.scr.2020.102078.
  PUBMED    33202307
REFERENCE   2  (bases 1 to 8935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2020-11-5"; volume: "49:102078. doi"; pages: " 
            10.1016/j.scr.2020.102078"
COMMENT     Sequence Label: p246 eSpCas9_2gRNAs_mH11
FEATURES             Location/Qualifiers
     source          1..8935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        270..345
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        436..684
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        705..780
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        870..1155
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        1157..1434
                     /label=chicken beta-actin promoter
     intron          1435..1662
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1674..1683
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1683..1748
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1755..1775
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1800..4337,4338..4340,4341..4802,4803..4805,4806..4973,
                     4974..4976,4977..5900)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5901..5948
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(5976..5993)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5982..6189
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6198..6338
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6198..6327
                     /label=AAV2 ITR
     rep_origin      6413..6868
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6500..6519)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6710..6731
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6885..6904)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7004..7026
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7064..7082)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7150..7254
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7255..7323,7324..8115)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7473..7492)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8286..8874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8775..8794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg aacactagtg cacttatccg ttttagagct agaaatagca agttaaaata
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag
      361 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag
      421 acaaatggct ctagagaggg cctatttccc atgattcctt catatttgca tatacgatac
      481 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
      541 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
      601 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      661 atcttgtgga aaggacgaaa caccgaaacc atatggcgct ccaggtttta gagctagaaa
      721 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      781 ttttttgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt tttagcgcgt
      841 gcgccaattc tgcagacaaa tggggtaccc gttacataac ttacggtaaa tggcccgcct
      901 ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc
      961 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     1021 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     1081 tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     1141 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     1201 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     1261 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     1321 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     1381 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     1441 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     1501 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     1561 tgtaattagc tgagcaagag gtaagggttt aagggatggt tggttggtgg ggtattaatg
     1621 tttaattacc tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca
     1681 tggactataa ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg
     1741 acgataagat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
     1801 acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca
     1861 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
     1921 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
     1981 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
     2041 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
     2101 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
     2161 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
     2221 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
     2281 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
     2341 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca
     2401 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc
     2461 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga
     2521 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg
     2581 ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga
     2641 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc
     2701 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga
     2761 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc
     2821 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct
     2881 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa
     2941 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc
     3001 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca
     3061 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga
     3121 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat
     3181 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg
     3241 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc
     3301 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata
     3361 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg
     3421 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga
     3481 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg
     3541 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca
     3601 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga
     3661 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc
     3721 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc
     3781 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt
     3841 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc
     3901 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg
     3961 agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga
     4021 aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg
     4081 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga
     4141 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg
     4201 aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata
     4261 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccatatcg
     4321 tgcctcagag ctttctggcc gacgactcca tcgacaacaa ggtgctgacc agaagcgaca
     4381 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact
     4441 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca
     4501 aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg
     4561 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta
     4621 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc
     4681 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc
     4741 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc
     4801 ctgcgctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga
     4861 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca
     4921 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aaggcgcctc
     4981 tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca
     5041 ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga
     5101 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca
     5161 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt
     5221 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag
     5281 agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc
     5341 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact
     5401 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga
     5461 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact
     5521 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc
     5581 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc
     5641 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca
     5701 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg
     5761 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg
     5821 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc
     5881 tgtctcagct gggaggcgac aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa
     5941 agaaaaagta agaattccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     6001 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6061 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6121 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca
     6181 tgctggggag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     6241 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     6301 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     6361 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     6421 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6481 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6541 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6601 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     6661 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6721 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     6781 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6841 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     6901 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     6961 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7021 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     7081 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7141 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7201 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7261 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7321 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7381 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7441 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7501 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7561 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7621 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7681 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7741 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7801 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7861 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7921 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg aagccgcggt
     7981 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8041 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8101 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8161 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8221 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8281 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8341 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8401 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8461 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8521 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8581 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8641 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8701 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8761 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8821 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8881 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
