LOCUS       sequence-326401-       10796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-inducible TIR1 expression vector for mouse H11 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10796)
  AUTHORS   Pryzhkova MV, Xu MJ, Jordan PW
  TITLE     Adaptation of the AID system for stem cell and transgenic mouse 
            research.
  JOURNAL   Stem Cell Res. 2020 Nov 5;49:102078. doi: 10.1016/j.scr.2020.102078.
  PUBMED    33202307
REFERENCE   2  (bases 1 to 10796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2020-11-5"; volume: "49:102078. doi"; pages: " 
            10.1016/j.scr.2020.102078"
COMMENT     Sequence Label: p281 TIR1_HA_flox STOP_mH11
FEATURES             Location/Qualifiers
     source          1..10796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(35..41,42..59,60..65))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    80..101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(218..235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(389..977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(469..488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1148..1939,1940..2008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1771..1790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2009..2113)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2140..2595
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2227..2246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2437..2458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2721..2743
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2735..2752
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2736..2752
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3557..4735
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3788..4726
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4683..4703
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(4781..4814)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          5348..5413
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5543..5563
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5988..6122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6164..6197)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6222..7946
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             7956..7982
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    8102..8223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8253..8286)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        8308..8807
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8343..8366)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8740..8759
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8847..9446
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8847..8866)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9343..9363
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(10015..10048)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
       61 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      121 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      181 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      241 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1141 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1201 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1261 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1321 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1381 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1441 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1501 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1561 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1621 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1681 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1741 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     1801 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     1861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     1921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     1981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2101 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     2161 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2221 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2281 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2341 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2401 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2461 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2521 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     2581 taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     2641 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     2701 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
     2761 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
     2821 aatcaatatt ggctattggc cattgcatac gtttaggact tctggaattt cttctgcaca
     2881 taaagttctg atagcattag tttaagctgg actaaccctg aaagtagctt gtggcaagta
     2941 tcaaggaatc aatattatac tctacaaaat caaagtttac agagaagtca tatagtaatt
     3001 tttctgaaat ttactggcac aatgttaatc cagcctgact ccaactaatt aatggtcaca
     3061 ttaatttaag tctttccctt gcctctgctg cattagtttc tctcaaaatt gttaacttac
     3121 aacttgaagt ctggtattat aaattgaatg taaagcattc tgaaagatac tatactgatt
     3181 gcaggttttt cagtcaggtt caagctaatt tgaccagtca ttggattaat tatggatctg
     3241 gggccataaa tgctatttta attccactat agagattaaa ataagccatt ctccatttca
     3301 taatattcta ttggactttg actgcagggg cctccaagtc ttgacagtag attataatcc
     3361 ttcagctgcc cactctactg gaggaggaca aactggtcac ttttcagcaa aacctggctg
     3421 tggatcaggg cagtctggta cttccaagct cattagatgc catcatgctc tcactgcctc
     3481 ctcagcttca agaggaatct ggaaaaagca gtcccactgg tcaggaaagg aacactagtg
     3541 cacaaacggc tcgaggggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     3601 ccccgagaag ttgtggggag gggtcggcaa ttgaagcggt gcctagagaa ggtggcgcgg
     3661 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     3721 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     3781 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     3841 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     3901 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     3961 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     4021 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     4081 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     4141 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     4201 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     4261 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     4321 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     4381 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     4441 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     4501 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     4561 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     4621 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     4681 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgagggga
     4741 tcgtcgatgt gctgtctcat cattttggca aagaattcta ataacttcgt atagcataca
     4801 ttatacgaag ttatattaag ggttccggat cctcggggac accaaatatg gcgatctcgg
     4861 ccttttcgtt tcttggagct gggacatgtt tgccatcgat ccatctacca ccagaacggc
     4921 cgttagatct gctgccaccg ttgtttccac cgaagaaacc accgttgccg taaccaccac
     4981 gacggttgtt gctaaagaag ctgccaccgc cacggccacc gttgtagccg ccgttgttgt
     5041 tattgtagtt gctactgtta tttctggcac ttcttggttt tcctcttaag tgaggaggaa
     5101 cataaccatt ctcgttgttg tcgttgatgc ttaaattttg cacttgttcg ctcagttcag
     5161 ccataatatg aaatgctttt cttgttgttc ttacggaata ccacttgcca cctatcacca
     5221 caactaactt tttcccgttc ctccatctct tttatatttt ttttctcgag ggatctttgt
     5281 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     5341 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     5401 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
     5461 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
     5521 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
     5581 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
     5641 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
     5701 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
     5761 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
     5821 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
     5881 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     5941 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     6001 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6061 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     6121 tctgacatgg taagtaagct tgggctgcag gtcgagggac ctaataactt cgtatagcat
     6181 acattatacg aagttatatg tcgacgccac tcgacgccac catgacctac ttccccgagg
     6241 aagtggtgga acacatcttc agctttctgc ccgcccagag ggacagaaac accgtgtccc
     6301 tcgtgtgcaa agtgtggtac gagatcgaga gactgagcag acggggcgtg ttcgtgggca
     6361 actgttacgc tgtgcgggct ggcagagtgg ccgccagatt tcctaatgtg cgggccctga
     6421 ccgtgaaggg caagcctcac ttcgccgact tcaacctggt gccccctgat tggggcggct
     6481 acgctggacc ttggatcgag gctgctgcca gaggctgtca cggcctggaa gaactgagaa
     6541 tgaagagaat ggtggtgtcc gacgagagcc tggaactgct ggccagaagc ttccccagat
     6601 tcagagccct ggtgctgatc tcctgcgagg gcttctctac agatggcctg gccgccgtgg
     6661 ccagccattg caagctgctg agagagctgg atctccagga aaacgaggtg gaagatagag
     6721 gccccagatg gctgagctgc ttccccgaca gctgtaccag cctggtgtcc ctgaacttcg
     6781 cctgcatcaa gggggaagtg aacgccggca gcctggaaag actggtgtcc agaagcccca
     6841 acctgagaag cctgagactg aacagatccg tgtccgtgga caccctggcc aagatcctgc
     6901 tgagaacccc taacctggaa gatctgggca ccggcaacct gaccgacgac ttccagaccg
     6961 agagctactt caagctgacc agcgccctgg aaaagtgcaa gatgctgcgg agcctgagcg
     7021 gcttctggga cgcctctcct gtgtgcctga gcttcatcta ccccctgtgc gcccagctga
     7081 ccggcctgaa cctgtcttac gcccctacac tggacgccag cgacctgacc aagatgatca
     7141 gcagatgcgt gaagctccag agactgtggg tgctggactg catcagcgac aagggactcc
     7201 aggtggtggc cagctcctgc aaggatctcc aggaactgag agtgttcccc agcgacttct
     7261 acgtggccgg ctacagcgcc gtgaccgaag agggactggt ggctgtgtct ctgggctgcc
     7321 ccaagctgaa cagcctgctg tacttctgcc accagatgac caacgccgcc ctcgtgaccg
     7381 tggccaagaa ctgccccaac ttcaccaggt tcagactgtg catcctggaa cccggcaagc
     7441 ccgacgttgt gaccagccag ccactggatg agggcttcgg agccatcgtg cgcgagtgca
     7501 agggactcca gagactgagc atcagcggcc tgctgaccga caaggtgttc atgtacatcg
     7561 ggaagtacgc caagcagctg gaaatgctgt ctatcgcctt cgctggcgac tccgacaagg
     7621 gcatgatgca cgtgatgaac ggctgcaaga acctgcggaa actggaaatc agagacagcc
     7681 ccttcggcga cgctgccctg ctgggcaact tcgccagata cgagacaatg agatccctgt
     7741 ggatgagcag ctgcaacgtg acactgaagg gctgccaggt gctggccagc aagatgccca
     7801 tgctgaacgt ggaagtgatc aacgagaggg acggcagcaa tgagatggaa gagaaccacg
     7861 gcgacctgcc taaggtggaa aagctgtacg tgtacagaac cacagccggc gctagggacg
     7921 acgcccctaa cttcgtgaag atcctgggcg gatcttaccc atacgacgta ccagattacg
     7981 cttaggcgat cgcctgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     8041 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     8101 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8161 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8221 ttaacgcgtc gccattttgg cagggaattc tagaagttcc tatactttct agagaatagg
     8281 aacttcactc gacctcgaaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     8341 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     8401 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     8461 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     8521 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     8581 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     8641 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     8701 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     8761 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc
     8821 tagatctcga gcagctgaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     8881 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     8941 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     9001 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     9061 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     9121 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     9181 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     9241 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     9301 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     9361 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     9421 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     9481 ggagcgcacg accccatgca tcgatgatat cagatccccg ggatgcagaa attgatgatc
     9541 tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact ttgttaagaa
     9601 gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg ggtgggggtg
     9661 gggtgggatt agataaatgc ctgctcttta ctgaaggctc tttactattg ctttatgata
     9721 atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg gccagctcat
     9781 tcctcccact catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg
     9841 attcccactt tgtggttcta agtactgtgg tttccaaatg tgtcagtttc atagcctgaa
     9901 gaacgagatc agcagcctct gttccacata cacttcattc tcagtattgt tttgccaagt
     9961 tctaattcca tcagaagctg gtcgagatcc taagcttggc tggacgtaaa ctcagaagtt
    10021 cctatacttt ctagagaata ggaacttcag gtaccgcggg cccgggatcc cccatatggt
    10081 ttctgtgaaa atgccttttc aatcattaac ctcttaaatg agtgttagca tcctaactaa
    10141 tgagtggtgc agaatagtgg gtctgcttag cttaactaag gccaagaaaa caaaacagga
    10201 aattcattcc atgtcatgag actcatacta cgaggttccc ttagacctca ggagaaaaag
    10261 tctttggctg taagaacaca cctcagtgga tgtggtagac tatgccttta ctcttttttt
    10321 tttttttttt tttttttggg ttttttcgag acagggtttc tctgtgtagc cttggctgtc
    10381 ctggaactca ctttgttgac caggctggcc tcgaactcag agatccgcct gcctctgcct
    10441 ctcaagtgct agaattaaaa gtgtgcgcca ccactcccag ctatatagac tatgccttta
    10501 atcaggacac ttgggaggca gggggatctc aaattcaatt ccagacagat gactgacaaa
    10561 cacacacata caccattcgt cttttctttt tttctttttt cttttgaaaa caggtttctc
    10621 tatgcagccc tggctttcct agaattcaat ctatagaccc agctggcctc aaacacagaa
    10681 cctcttgcct ctgcctcctc aacactatga ctaaaggtgt gaaccaccac caccaccacc
    10741 cacctggcca aagaatgaat tgagtgaata agtacgagtg caactctcca gggccg
//
