LOCUS       sequence-326557-        2482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0410
FEATURES             Location/Qualifiers
     source          1..2482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     74..92
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(96..139)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      597..654
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(678..695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(849..1437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(929..948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1620..1714)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1735..2394)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2309..2328
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac
       61 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc
      121 tttcgctaag gatgatttct ggaattcggt ctcaggaggg aggcgtgtcg ttttagtgag
      181 gatcgaaagg acagtaactg ctaggaggac cgatgctgat cttccatcaa caaataatcc
      241 aagtaaggta ggtcacgacc actataattt cgggaaacct cctcggctat cgctttagca
      301 agacctctac attgcgataa aggaaaggct atgcccaatt atgacccccg ccgatgacgc
      361 gggaaaaact ggtacttgtt gaagcatctt ccactatgcc caatggtgga gcacgacaaa
      421 actggtactt gtgtacagct gacgtaaggg atgacgcaca tatcagctta gcaagacctc
      481 ttgaagataa gataataatg ttgaagataa gatgcctgcc ttgatgacta tataaggttt
      541 tgctattcat tgaaagcagt agtgactgat ttgtatatat actcgagacc ctgcagtccg
      601 gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt
      661 cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      841 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1261 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1501 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1561 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga
     1621 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     1681 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     1741 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     1801 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     1861 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1921 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     1981 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     2041 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     2101 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     2161 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     2221 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     2281 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     2341 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     2401 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     2461 attatggtga aagttggaac ct
//
