LOCUS       sequence-326559-        2350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0416
FEATURES             Location/Qualifiers
     source          1..2350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(152..246)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(267..926)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     841..860
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1088..1106
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1110..1153)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1479..1536
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1560..1577)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1731..2319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1811..1830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
       61 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      121 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc
      181 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
      241 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
      301 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
      361 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
      421 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
      481 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
      541 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
      601 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
      661 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
      721 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
      781 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
      841 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
      901 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
      961 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     1021 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     1081 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     1141 taaggatgat ttctggaatt cggtctcagg agggaggcgt gtcgttttag tgagggtggg
     1201 agccaccaca catgtaggct atcagcttag caatcgaaag gacagtatgt gtacagggct
     1261 cactagcaag tggataaact ggtacttgtg tatgaagata agataataat gttgaagata
     1321 agatagcaag acctctacaa ctgacgtaag ggatgacgca cactgctagg aggacatgac
     1381 gtaagccatg acgtctaccg atgatgatcc tatataaggt tttgctattc attgaaagca
     1441 gtagtgactg atttgtatat atactcgaga ccctgcagtc cggcaaaaaa gggcaaggtg
     1501 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc
     1561 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1621 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1681 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
     1741 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1801 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1861 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1921 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1981 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2041 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2101 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2161 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2221 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2281 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2341 acggggtctg
//
