LOCUS       sequence-326566-        6027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 507 / 583, Emission: 517 / 
            593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, 
            Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, 
            Davidson MW, Fetter RD, Hess HF
  TITLE     Interferometric fluorescent super-resolution microscopy resolves 3D 
            cellular ultrastructure.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30. doi: 
            10.1073/pnas.0813131106. Epub 2009 Feb 6.
  PUBMED    19202073
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0813131106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-3- 3"; volume: "106"; issue: "9"; pages: "3125-30"
COMMENT     Sequence Label: mKikGR-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..6027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             169..963
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             169..963
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(223..242)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     833..852
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1151..1170)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1195..1242
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1571..2159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2060..2079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2334..2637
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2638..2841
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2792..2812
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2880..2889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2886..3584
                     /codon_start=1
                     /product="monomeric photoconvertible Kikume green-to-red 
                     fluorescent protein"
                     /label=mKikGR1
                     /translation="MVSVITSEMKIELRMEGSVNGHKFVITGKGSGRPYEGTQTVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQLFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNTFVNEIRFDGTNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVEM
                     ALLLEGGGHYRCDFRTTYKAKKVVQLPDYHYVDHQMEITSHDKDYNKVKAYEHAKAYSG
                     TYRGAKYEFEA"
     polyA_signal    5088..5209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5125..5144)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5179..5198
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5216..5671)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5353..5374)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5565..5584
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5698..5802
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(5804..6027,1..134)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5804..5995
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5827..5847)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(6012..6027,1..120)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
       61 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
      121 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
      181 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      241 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      301 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
      361 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      421 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      481 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      541 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      601 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      661 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
      721 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
      781 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
      841 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
      901 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
      961 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     1021 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     1081 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     1141 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     1201 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     1261 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     1321 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     1381 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     1441 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1501 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1561 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1621 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1681 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1741 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1801 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1981 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2041 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2101 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2161 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2221 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat
     2281 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     2341 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2401 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2461 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2521 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2581 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2641 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2701 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2761 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2821 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg
     2881 ccaccatggt gagtgtgatt acatcagaaa tgaagatcga gctgcgtatg gaaggctctg
     2941 taaacgggca caagttcgtg attacaggga aaggaagtgg cagaccttac gagggaacac
     3001 agactgtgga cctgacagtc atagagggcg gacctcttcc ttttgctttc gatatcctga
     3061 caacagcatt ccattacggc aaccgggtat ttgtcgaata cccagaagaa atagtagact
     3121 acttcaagca gttgtttcct gagggttatt cttgggaacg aagcatgagt tacgaagacg
     3181 ggggaatttg cctcgccaca aacaatataa cgatgaagaa agacggcagc aacacttttg
     3241 tcaatgaaat tcgatttgat ggtacaaact ttcctgccaa tggtccagtt atgcagagga
     3301 agaccgtcaa atgggagcca tccactgaga aaatgtatgt gcgtgatgga gtgctgaagg
     3361 gtgatgttga aatggctctg ttgcttgaag gaggtggcca ttaccgatgt gacttcagaa
     3421 ctacttacaa agcaaagaag gttgtccagt tgccagacta tcactacgtg gatcatcaaa
     3481 tggagataac aagccatgac aaggattaca acaaggttaa ggcgtatgaa catgctaagg
     3541 cttattccgg gacctacagg ggcgccaagt atgaatttga agcctccgga ctcagatctc
     3601 gagtgcgtga gtgcatctcc atccacgttg gccaggctgg tgtccagatt ggcaatgcct
     3661 gctgggagct ctactgcctg gaacacggca tccagcccga tggccagatg ccaagtgaca
     3721 agaccattgg gggaggagat gactccttca acaccttctt cagtgagacg ggcgctggca
     3781 agcacgtgcc ccgggctgtg tttgtagact tggaacccac agtcattgat gaagttcgca
     3841 ctggcaccta ccgccagctc ttccaccctg agcagctcat cacaggcaag gaagatgctg
     3901 ccaataacta tgcccgaggg cactacacca ttggcaagga gatcattgac cttgtgttgg
     3961 accgaattcg caagctggct gaccagtgca ccggtcttca gggcttcttg gttttccaca
     4021 gctttggtgg gggaactggt tctgggttca cctccctgct catggaacgt ctctcagttg
     4081 attatggcaa gaagtccaag ctggagttct ccatttaccc agcaccccag gtttccacag
     4141 ctgtagttga gccctacaac tccatcctca ccacccacac caccctggag cactctgatt
     4201 gtgccttcat ggtagacaat gaggccatct atgacatctg tcgtagaaac ctcgatatcg
     4261 agcgcccaac ctacactaac cttaaccgcc ttattagcca gattgtgtcc tccatcactg
     4321 cttccctgag atttgatgga gccctgaatg ttgacctgac agaattccag accaacctgg
     4381 tgccctaccc ccgcatccac ttccctctgg ccacatatgc ccctgtcatc tctgctgaga
     4441 aagcctacca tgaacagctt tctgtagcag agatcaccaa tgcttgcttt gagccagcca
     4501 accagatggt gaaatgtgac cctcgccatg gtaaatacat ggcttgctgc ctgttgtacc
     4561 gtggtgacgt ggttcccaaa gatgtcaatg ctgccattgc caccatcaaa accaagcgca
     4621 gcatccagtt tgtggattgg tgccccactg gcttcaaggt tggcatcaac taccagcctc
     4681 ccactgtggt gcctggtgga gacctggcca aggtacagag agctgtgtgc atgctgagca
     4741 acaccacagc cattgctgag gcctgggctc gcctggacca caagtttgac ctgatgtatg
     4801 ccaagcgtgc ctttgttcac tggtacgtgg gtgaggggat ggaggaaggc gagttttcag
     4861 aggcccgtga agatatggct gcccttgaga aggattatga ggaggttggt gtggattctg
     4921 ttgaaggaga gggtgaggaa gaaggagagg aatactaagg atccaccgga tctagataac
     4981 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     5041 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     5101 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5161 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt
     5221 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     5281 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     5341 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     5401 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     5461 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     5521 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     5581 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     5641 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     5701 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5761 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     5821 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5881 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5941 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6001 taactccgcc catcccgccc ctaactc
//
