LOCUS       sequence-326573-        2718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive murine mature BDNF expression, controlled under E.coli 
            tet promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Toparlak OD, Zasso J, Bridi S, Serra MD, Macchi P, Conti L, Baudet 
            ML, Mansy SS
  TITLE     Artificial cells drive neural differentiation.
  JOURNAL   Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 
            10.1126/sciadv.abb4920. Print 2020 Sep.
  PUBMED    32948587
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-09-18"; volume: "6(38). pii: 6/38/eabb4920. doi"; pages:
            " 10.1126/sciadv.abb4920. Print 2020 Sep"
COMMENT     Sequence Label: DT049A
FEATURES             Location/Qualifiers
     source          1..2718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..34
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(38..81)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    84..103
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     protein_bind    105..123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    130..148
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             187..210
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      696..743
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    745..765
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(771..788)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(942..1530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1022..1041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1701..2492,2493..2561))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2324..2343
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2562..2666)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
       61 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagatcccta tcagtgatag
      121 agattgacat ccctatcagt gatagagata ctgagcacga tactagaaga aggagaataa
      181 tctatggact acaaagacga tgatgacaag atgcactccg accctgcccg ccgtggggag
      241 ctgagcgtgt gtgacagtat tagcgagtgg gtcacagcgg cagataaaaa gactgcagtg
      301 gacatgtctg gcgggacggt cacagtccta gagaaagtcc cggtatccaa aggccaactg
      361 aagcagtatt tctacgagac caagtgtaat cccatgggtt acaccaagga aggctgcagg
      421 ggcatagaca aaaggcactg gaactcgcaa tgccgaacta cccaatcgta tgttcgggcc
      481 cttactatgg atagcaaaaa gagaattggc tggcgattca taaggataga cacttcctgt
      541 gtatgtacac tgaccattaa gaggggaaga tagtagcgac tcaggctgct acgcctgtgt
      601 actggaaaac aaaaccaaaa cccaaaaaac aaaaaactga gcccattggt atcgtggaag
      661 gactcgatca aaaaaaaaaa aaaaaaaaaa aaaaactagc ataacccctt ggggcctcta
      721 aacgggtctt gaggggtttt ttggtactag tagcggccgc tgcaggcttc ctcgctcact
      781 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      841 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      901 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
      961 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1021 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1081 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1141 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1201 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1261 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1321 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1381 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1441 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1501 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1561 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1621 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1681 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1741 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1801 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     1861 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1921 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1981 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     2041 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     2101 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     2161 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     2221 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     2281 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2341 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     2401 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     2461 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     2521 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     2581 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2641 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     2701 gaaaccatta ttatcatg
//
