LOCUS       sequence-326577-        4890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive LuxR expression, controlled under J23101 E.coli 
            promoter, and inducible Perfringolysin O (PFO) expression, 
            controlled under V. fischeri HSL-regulated pLux (luxPR) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Toparlak OD, Zasso J, Bridi S, Serra MD, Macchi P, Conti L, Baudet 
            ML, Mansy SS
  TITLE     Artificial cells drive neural differentiation.
  JOURNAL   Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 
            10.1126/sciadv.abb4920. Print 2020 Sep.
  PUBMED    32948587
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-09-18"; volume: "6(38). pii: 6/38/eabb4920. doi"; pages:
            " 10.1126/sciadv.abb4920. Print 2020 Sep"
COMMENT     Sequence Label: DT048A
FEATURES             Location/Qualifiers
     source          1..4890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     273..292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(511..615)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     683..701
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(705..748)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    751..770
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        772..806
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     CDS             835..858
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      1738..1785
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1878..1901
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      3535..3582
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    3584..3604
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(3610..3627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3781..4369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3861..3880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4540..4890,1..441,442..510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
       61 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
      181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
      241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
      301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      361 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
      421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
      481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
      541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
      601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
      661 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
      721 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag atttacagct
      781 agctcagtcc taggtattat gctagcgata ctagaagaag gagaataatc tatggactac
      841 aaagacgatg atgacaagat gaaaaacata aatgccgacg acacatacag aataattaat
      901 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      961 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
     1021 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
     1081 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
     1141 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
     1201 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
     1261 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
     1321 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
     1381 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
     1441 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
     1501 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
     1561 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacgactc
     1621 aggctgctac gcctgtgtac tggaaaacaa aaccaaaacc caaaaaacaa aaaactgagc
     1681 ccattggtat cgtggaagga ctcgatcaaa aaaaaaaaaa aaaaaaaaaa aaaaaaacta
     1741 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttggtact agaacctgta
     1801 ggatcgtaca ggtttacgca agaaaatggt ttgttatagt cgaataaaaa atactagaag
     1861 aaggagaata atctatggac tacaaagacg atgatgacaa gatgaaagat atcacggata
     1921 aaaaccaatc gattgatagt ggcatcagtt cgctgagcta taaccgcaat gaagtcctgg
     1981 ccagcaatgg tgataaaatt gaatcgtttg ttcctaaaga gggtaaaaaa gcgggcaata
     2041 aattcattgt agtcgaacgc cagaaacgta gcctgactac ttcaccggtt gatatttcca
     2101 ttattgacag tgttaatgat cgcacgtatc ctggtgcgct gcaactggct gataaagcat
     2161 ttgtcgaaaa tcgccctact attctgatgg taaaacgtaa accgatcaat atcaacattg
     2221 atctgccggg tctgaaaggt gaaaactcta tcaaggtgga tgatccaact tatggtaaag
     2281 tcagcggcgc cattgatgaa ctggttagca aatggaatga aaaatatagc agcacccaca
     2341 ccctgccggc gcgcacccag tactccgaat ccatggtgta tagtaaatcc caaatctcat
     2401 cggccctgaa tgtgaacgct aaagtgctgg aaaattcgct gggtgttgac ttcaacgccg
     2461 tagcaaataa cgagaaaaaa gtaatgatcc tggcatataa gcagatcttt tatacagtca
     2521 gtgccgatct gccgaaaaat ccgtctgatc tgttcgatga tagcgttact tttaatgatc
     2581 tgaagcaaaa aggtgttagc aacgaagctc cgccgctgat ggtttctaat gttgcctatg
     2641 gtcgcaccat ttatgttaaa ctggaaacca ctagctcctc gaaagatgtc caagctgctt
     2701 ttaaagctct gatcaaaaat acagatatta aaaatagtca acagtataaa gatatttatg
     2761 aaaatagcag tttcacagca gttgtgctgg gaggggatgc acaggaacat aataaagtgg
     2821 tgacgaaaga ttttgacgaa atccgcaaag tcatcaaaga taatgccact tttagtacga
     2881 agaatccggc ttatccaatc agctatacct ctgtcttcct gaaagacaac agcgttgccg
     2941 ccgtccataa caagaccgat tacattgaaa ccaccagtac cgaatattct aaaggtaaaa
     3001 tcaacctgga tcattccggt gcgtatgtcg ctcagtttga agtggcgtgg gacgaggtct
     3061 catacgataa agaaggtaac gaagttctga cgcataaaac ttgggacggc aattaccagg
     3121 acaaaactgc ccactatagc accgttatcc cgctggaagc caatgcgcgc aacatccgca
     3181 ttaaagcacg tgaagcaacg gggctggcct gggaatggtg gcgtgatgta attagcgaat
     3241 atgatgtgcc gctgacgaat aatatcaacg tttcgatttg ggggaccacc ctgtatccgg
     3301 gttcaagcat tacctataat tgatgacgac tcaggctgct acgcctgtgt actggaaaac
     3361 aaaaccaaaa cccaaaaaac aaaaaactsa rscmwtwsst ataattgatg acgactcagg
     3421 ctgctacgcc tgtgtactgg aaaacaaaac caaaacccaa aaaacaaaaa actgagccca
     3481 ttggtatcgt ggaaggactc gatcaaaaaa aaaaaaaaaa aaaaaaaaaa aaaactagca
     3541 taaccccttg gggcctctaa acgggtcttg aggggttttt tggtactagt agcggccgct
     3601 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3661 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3721 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3781 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3841 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3901 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3961 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4021 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4081 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4141 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4201 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4261 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4441 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4501 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4561 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4621 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4681 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4741 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4801 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4861 catcgtggtg tcacgctcgt cgtttggtat
//
