LOCUS       sequence-326581-       13527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13527)
  AUTHORS   Roy S, Torres-Jerez I, Zhang S, Liu W, Schiessl K, Jain D, Boschiero
            C, Lee HK, Krom N, Zhao PX, Murray JD, Oldroyd GED, Scheible WR, 
            Udvardi M
  TITLE     The peptide GOLVEN10 alters root development and noduletaxis in 
            Medicago truncatula.
  JOURNAL   Plant J. 2024 May;118(3):607-625. doi: 10.1111/tpj.16626. Epub 2024 
            Feb 15.
  PUBMED    38361340
REFERENCE   2  (bases 1 to 13527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16626";
            journalName: "Plant J"; date: "2024-05"; volume: "118"; issue: "3"; 
            pages: "607-625"
COMMENT     Sequence Label: LjUBQ:MtGLV9:T35s
FEATURES             Location/Qualifiers
     source          1..13527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(140..162)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    248..388
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     303..322
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(403..420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(574..1162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(654..673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1408..2199)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    2720..2744
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(2837..3089)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3287..4312)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      complement(4628..4880)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(4893..5567,5568..5570,5571..5573))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MARSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(4955..4978)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5153..5172)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5370..5390
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     misc_feature    9685..9709
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11040..11669
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12098..13171
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13237..13431
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
       61 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
      121 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
      181 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      241 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
      301 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
      361 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
      421 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      481 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     1021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1201 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat gatatatctc ccaatttgtg
     1261 tagggcttat tatgcacgct taaaaataat aaaagcagac ttgacctgat agtttggctg
     1321 tgagcaatta tgtgcttagt gcatctaacg cttgagttaa gccgcgccgc gaagcggcgt
     1381 cggcttgaac gaatttctag ctagacatta tttgccgact accttggtga tctcgccttt
     1441 cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
     1501 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     1561 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     1621 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     1681 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     1741 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     1801 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     1861 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     1921 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     1981 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     2041 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
     2101 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     2161 tagttgagtc gatacttcgg cgatcaccgc ttcccccatg atgtttaact ttgttttagg
     2221 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
     2281 gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa aacagtcata
     2341 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
     2401 ccagttgcgt gagcgcatac gctacttgca ttacagctta cgaaccgaac aggcttatgt
     2461 ccactgggtt cgtgcccgaa ttgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     2521 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     2581 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
     2641 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     2701 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
     2761 aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttgaattatc
     2821 agcttgcatg ccggtcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     2881 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     2941 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     3001 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     3061 aaactttatt gccaaatgtt tgaacgatct gcttgactct agggaattaa ttcctgaatc
     3121 actgcgaccg gccctcccgc gacccagccg agcgagctta gcgaactgtg gacgagaact
     3181 gtgccaccaa gcgtaaggcc gttctctcgc atttgccttg ctaggctcgc gcgagttgct
     3241 ggctgaggcg ttctcgaaat cagctcttgt tcggtcggca tctactctat tcctttgccc
     3301 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
     3361 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
     3421 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
     3481 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc cttggcgatc
     3541 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
     3601 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
     3661 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
     3721 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
     3781 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
     3841 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
     3901 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgata gcatccatag
     3961 cctccgcgac cggctgaaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
     4021 caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaactcc ccaatgtcaa
     4081 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     4141 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     4201 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
     4261 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atagggggga
     4321 ttcgagttga gagtgaatat gagactctaa ttggataccg aatttatgga acgtcagtgg
     4381 agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg
     4441 caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctcagt
     4501 ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat
     4561 cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcgataa ttcgtcgacg
     4621 gcgcgccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
     4681 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
     4741 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
     4801 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
     4861 tgccaaatgt ttgaacgatc gggaattgga tcctacagga acaggtggtg gcggccctcg
     4921 gtgcgctcgt actgctccac gatggtgtag tcctcgttgt gggaggtgat gtccagcttg
     4981 gagtccacgt agtagtagcc gggcagctgc acgggcttct tggccatgta gatggacttg
     5041 aactccacca ggtagtggcc gccgtccttc agcttcaggg ccttgtggat ctcgcccttc
     5101 agcacgccgt cgcgggggta caggcgctcg gtggaggcct cccagcccat ggtcttcttc
     5161 tgcattacgg ggccgtcgga ggggaagttc acgccgatga acttcacctt gtagatgaag
     5221 cagccgtcct gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc
     5281 atcacgcgct cccacttgaa gccctcgggg aaggacagct tcttgtagtc ggggatgtcg
     5341 gcggggtgct tcacgtacac cttggagccg tactggaact ggggggacag gatgtcccag
     5401 gcgaagggca gggggccgcc cttggtcacc ttcagcttca cggtgttgtg gccctcgtag
     5461 gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacggt gccctccatg
     5521 cgcaccttga agcgcatgaa ctccttgatg acgttcttgg aggagcgcgc catggcaaag
     5581 atctgcatct gttaatcaga aaaactcaga ttaatcgaca aattcgatcg cacaaactag
     5641 aaactaacac cagatctaga tagaaatcac aaatcgaaga gtaattattc gacaaaactc
     5701 aaattatttg aacaaatcgg atgatatcta tgaaacccta atcgagaatt aagatgatat
     5761 ctaacgatca aacccagaaa atcgtcttcg atctaagatt aacagaatct aaaccaaaga
     5821 acatatacga aattgggatc gaacgaaaac aaaatcgaag attttgagag aataaggaac
     5881 acagaaattt accttgatca cggtagagag aattgagaga aagtttttaa gattttgaga
     5941 aattgaaatc tgaattgtga agaagaagag ctctttgggt attgttttat agaagaagaa
     6001 gaagaaaaga cgaggacgac taggtcacga gaaagctaag gcggtgaagc aatagctaat
     6061 aataaaatga cacgtgtatt gagcgttgtt tacacgcaaa gttgtttttg gctaattgcc
     6121 ttatttttag gttgaggaaa agtatttgtg ctttgagttg ataaacacga ctcgtgtgtg
     6181 ccggctgcaa ccactttgac gccgtttatt actgactcgt cgacaaccac aatttctaac
     6241 ggtcgtcata agatccagcc gttgagattt aacgatcgtt acgatttata tttttttagc
     6301 attatcgttt tattttttaa atatacggtg gagctgaaaa ttggcaataa ttgaaccgtg
     6361 ggtcccactg cattgaagcg tatttcgtat cttctagaat tcttcgtgct ttatttcttt
     6421 tcctttttgt ttttttttgc catttatcta atgcaagtgg gcttataaaa tcagtgaatt
     6481 tcttggaaaa gtaacttctt tatcgtataa catattgtga aattatccat ttcttttaat
     6541 tttttagtgt tattggatat ttttgtatga ttattgattt gcataggata atgacttttg
     6601 tatcaagttg gtgaacaagt ctcgttaaaa aaggcaagtg gtttggtgac tcgatttatt
     6661 cttgttattt aattcatata tcaatggatc ttatttgggg cctggtccat atttaacact
     6721 cgtgttcagt ccaatgacca ataatatttt ttcattaata acaatgtaac aagaatgata
     6781 cacaaaacat tctttgaata agttcgctat gaagaaggga acttatccgg tcctagatca
     6841 tcagttcata caaacctcca tagagttcaa catcttaaac aagaatatcc tgatctgaag
     6901 aatgtggagg ctttagtccc ttggatactt gggaggctgt ggaagaacag aaacgagctg
     6961 gtgcttaaag ggagggaatt tggaaccaat gaggtattag taaggacaca agaagatgca
     7021 gatgagtgga ttagaaggaa agaggctcag aatgtaagga aagcaccaac cacaacggcg
     7081 cgcccgggag agaggatttt gaggaaataa ttaattgaat tacttgtatt attgataaag
     7141 taatttagat aagttgttag tacaacttat tgcaactatg gatagacaaa aatcacttat
     7201 tttaagggga gaagtatata acaacttatt ataaaatttc tgagcagccg gcctcctcca
     7261 atcaatcata gagagtggag cccattctgg aaaaccaagg aacccccaac ttgcactggt
     7321 gcggtggccc aattcaaaaa aaacggggcc cagcaaagta acgccgttgt aacgatttat
     7381 caatccaaac tcaaaaggcg gcgagatgcg tttaactccg tgaaattaac aaaccgccaa
     7441 caacttgcaa tttgcaacta ccgtttccaa gaagaactca accacacaac gtatcctatc
     7501 ccaaaccaca cgcaactagt gacgcgtcat aaggacacgt gtcacaattt gactggttaa
     7561 taatttcacc gcttttgcta taaattacct ccaatcccct tagcttcttc acaattcagt
     7621 tcccaaccct aacaattctt gttcatatcg cttctctcta ctttcaaggt atgatccaat
     7681 ttctctcttc tttctctgta atcctttcgt tgagttttgt ttccgatcaa tcataggtag
     7741 ttttcttgtt tcgaagcatg agatctagga attttttgtg attttccaaa attgagatcg
     7801 gtttgaaatt gaattttaca gcttgaatct cagatcttgt tttatcaatg ttttcgatgg
     7861 cttgcgatgt agatctatga taattgtggt tcagttttgt taggaatcga tttcggttta
     7921 gcaattgcag attaattagg gtttccaatt gaattcttca gatccgttat ggaattatgt
     7981 caaataattt attcaaattg gaaattattg ttagatccac tcttaatctg tttgatccaa
     8041 gcttcaatta gggttttcac ttgtttcaat ttcttgttat ggattctgat ttatctgttg
     8101 atgttagatc cactcttaat ctgtttgatc caagcgttaa ttagggtttt cacttgtttc
     8161 aatttcgtgt gttggattct gatttatctg ttgttgatgt gattacagat gaagccttct
     8221 tgtgtagtag tttctcttct cattttgtca cttctcctta caaaaactca aggtaaatat
     8281 ataaatataa tttaacagca tgcagttcaa acaataatta actagtcctt gttacatttc
     8341 taatcttcct tttctgtttg tgtgcaggaa tacgtttggg aaaagggtct ttagcatttg
     8401 agcaacaaaa acaacatgta ggatattatc tactttgaag ttttatgtat ttaaaccttt
     8461 catatttatg catgctcacg tttcaatgga tgttgatata gaagttcact tttctgactg
     8521 caggatgagg aaagaagtac tatgttgaaa agaagcaaca ctgattctga ggaagctact
     8581 ctttgcaatg accaacaaaa atgcacaggt agcttgaaga aaacagcttc taaagtatcc
     8641 agaaaatcac acaaccagtt accacagatt catgaagatt actatggacc tagacgacat
     8701 agatctagtc accattagtt ttttaagtcc acaagttttt cttccttttt tctttataaa
     8761 aaaattaggg tagtggaaaa ttattatcta aagtggcgcg aatgaccatt ttacaattgt
     8821 agatttgaac ttcgtccttt gaaattacaa ggacatattt ttaccactaa gctgacaaat
     8881 tttgtcccaa aaccttatga ctatgcaaaa atgcaaggct ttattctctt ctcttggtat
     8941 atctggttta aaacatagta taaaatatat ttatacaata aagcatcttt catatataaa
     9001 tcatagtatt aattaagcat tacaaatgtt tttattttgc tgcaggaaat ataaagaata
     9061 ggaaacttgt taccacttca atttctacaa ctaaatcctt gtcaaaggtt ggttataaga
     9121 gatcaaataa attatatcat aaaccttgga atctattttt tttttacatc tttatttata
     9181 ataattgtat atatgcttca tgaacagaat gtgaagaaca gtgaggatgt ggctcacacc
     9241 tcagtaaatg gcaatactag aaatgtgaat ctgaatgggg aagcaaagga aataaaagta
     9301 aaatcattga gtacttctaa ttatctccct gaggaccttg tagacataac tgagatggat
     9361 tattcaccag ctaggaaaaa gtctcccata cacaattaac ggccatgcta gagtccgcaa
     9421 aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg
     9481 agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata
     9541 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     9601 tcctaaaacc aaaatccagt gaaagcttag cttgagcttg gatcagattg tcgtttcccg
     9661 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     9721 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     9781 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt taaagtactt
     9841 taaagtactt taaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     9901 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     9961 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
    10021 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
    10081 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
    10141 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
    10201 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
    10261 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
    10321 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
    10381 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
    10441 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
    10501 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
    10561 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
    10621 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
    10681 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
    10741 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
    10801 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
    10861 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
    10921 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
    10981 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
    11041 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
    11101 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
    11161 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
    11221 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
    11281 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
    11341 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
    11401 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
    11461 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
    11521 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
    11581 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
    11641 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
    11701 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
    11761 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
    11821 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
    11881 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
    11941 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
    12001 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct
    12061 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
    12121 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
    12181 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
    12241 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
    12301 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
    12361 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
    12421 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
    12481 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
    12541 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
    12601 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
    12661 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
    12721 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
    12781 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
    12841 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
    12901 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
    12961 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
    13021 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
    13081 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
    13141 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
    13201 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
    13261 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
    13321 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
    13381 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
    13441 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
    13501 ccccgccgct tcgcgtcggc ctatcgc
//
