LOCUS       sequence-326593-       10277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitously expresses rtTAM2, dexamethasone-inducible human SOX17, 
            and hygromycin-resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Yoshimatsu S, Nakajima M, Iguchi A, Sanosaka T, Sato T, Nakamura M, 
            Nakajima R, Arai E, Ishikawa M, Imaizumi K, Watanabe H, Okahara J, 
            Noce T, Takeda Y, Sasaki E, Behr R, Edamura K, Shiozawa S, Okano H
  TITLE     Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts
            of Multiple Mammalian Species.
  JOURNAL   Stem Cell Reports. 2021 Mar 23. pii: S2213-6711(21)00132-6. doi: 
            10.1016/j.stemcr.2021.03.002.
  PUBMED    33798453
REFERENCE   2  (bases 1 to 10277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2021 Mar 23. pii: S2213-6711(21)00132-6. doi: 
            10.1016/j.stemcr.2021.03.002."
COMMENT     Sequence Label: pPBCAG-rtTAM2-2A-SOX17-GR-IH
FEATURES             Location/Qualifiers
     source          1..10277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          587..1603
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1527..1549
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1611..1630
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1670..2287,2288..2293,2294..2329,2330..2332,2333..2368,
                     2369..2371,2372..2407,2408..2410)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLP"
     CDS             2411..2464
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3413..3430
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             3419..3436
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             4559..4582
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    4603..5176
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4770..4787)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4997..5016
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5215..6234
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5215..6234
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     polyA_signal    6642..6763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6679..6698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6733..6752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6952..7014
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7038..7056)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7077..7093)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7077..7093)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7090..7112)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7101..7117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7125..7131,7132..7149,7150..7155))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7170..7191
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7308..7325)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7479..8067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7559..7578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8238..9029,9030..9098))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8861..8880
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9099..9203)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9229..9684)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9366..9387)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9578..9597
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9811..9833
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9825..9842
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9826..9842
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9849..9868
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9849..9867
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   9886..9920
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        join(10198..10277,1..304)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
      361 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
      421 gggggggggg cgcgcgccrg gggggggggg gggggggggg gggggggggg gggsgrggsg
      481 grgrggkgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
      541 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
      601 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
      661 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
      721 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
      781 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
      841 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
      901 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
      961 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     1021 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     1081 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     1141 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     1201 cggggrgggc tcgggggrgg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
     1261 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     1321 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     1381 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     1441 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     1501 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     1561 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     1621 ggttattgtg ctgtctcatc attttggcaa agaattcctc gaggccacca tgtctagact
     1681 ggacaagagc aaagtcataa acggcgctct ggaattactc aatggagtcg gtatcgaagg
     1741 cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca
     1801 cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca
     1861 tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa
     1921 gtcattccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg
     1981 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg
     2041 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg
     2101 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga
     2161 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc
     2221 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
     2281 aagcggcggg ccggccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc
     2341 ccttgacgac tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga
     2401 catgctcccc gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg
     2461 ccccatgagc agcccggatg cgggatacgc cagtgacgac cagagccaga cccagagcgc
     2521 gctgcccgcg gtgatggccg ggctgggccc ctgcccctgg gccgagtcgc tgagccccat
     2581 cggggacatg aaggtgaagg gcgaggcgcc ggcgaacagc ggagcaccgg ccggggccgc
     2641 gggccgagcc aagggcgagt cccgtatccg gcggccgatg aacgctttca tggtgtgggc
     2701 taaggacgag cgcaagcggc tggcgcagca gaatccagac ctgcacaacg ccgagttgag
     2761 caagatgctg ggcaagtcgt ggaaggcgct gacgctggcg gagaagcggc ccttcgtgga
     2821 ggaggcagag cggctgcgcg tgcagcacat gcaggaccac cccaactaca agtaccggcc
     2881 gcggcggcgc aagcaggtga agcggctgaa gcgggtggag ggcggcttcc tgcacggcct
     2941 ggctgagccg caggcggccg cgctgggccc cgagggcggc cgcgtggcca tggacggcct
     3001 gggcctccag ttccccgagc agggcttccc cgccggcccg ccgctgctgc ctccgcacat
     3061 gggcggccac taccgcgact gccagagtct gggcgcgcct ccgctcgacg gctacccgtt
     3121 gcccacgccc gacacgtccc cgctggacgg cgtggacccc gacccggctt tcttcgccgc
     3181 cccgatgccc ggggactgcc cggcggccgg cacctacagc tacgcgcagg tctcggacta
     3241 cgctggcccc ccggagcctc ccgccggtcc catgcacccc cgactcggcc cagagcccgc
     3301 gggtccctcg attccgggcc tcctggcgcc acccagcgcc cttcacgtgt actacggcgc
     3361 gatgggctcg cccggggcgg gcggcgggcg cggcttccag atgcagccgc aacaccagca
     3421 ccagcaccag caccagcacc accccccggg ccccggacag ccgtcgcccc ctccggaggc
     3481 actgccctgc cgggacggca cggaccccag tcagcccgcc gagctcctcg gggaggtgga
     3541 ccgcacggaa tttgaacagt atctgcactt cgtgtgcaag cctgagatgg gcctccccta
     3601 ccaggggcat gactccggtg tgaatctccc cgacagccac ggggccattt cctcggtggt
     3661 gtccgacgcc agctccgcgg tatattactg caactatcct gacgtggggt ctggtattca
     3721 gcaggccact acaggagtct cacaagaaac ctctgaaaat cctggtaaca aaacaatagt
     3781 tcctgcaacg ttaccacaac tcacccctac cctggtgtca ctgttggagg ttattgaacc
     3841 tgaagtgtta tatgcaggat atgatagctc tgttccagac tcaacttgga ggatcatgac
     3901 tacgctcaac atgttaggag ggcggcaagt gattgcagca gtgaaatggg caaaggcaat
     3961 accaggtttc aggaacttac acctggatga ccaaatgacc ctactgcagt actcctggat
     4021 gtttcttatg gcatttgctc tggggtggag atcatataga caatcaagtg caaacctgct
     4081 gtgttttgct cctgatctga ttattaatga gcagagaatg actctaccct gcatgtacga
     4141 ccaatgtaaa cacatgctgt atgtttcctc tgagttacac aggcttcagg tatcttatga
     4201 agagtatctc tgtatgaaaa ccttactgct tctctcttca gttcctaagg acggtctgaa
     4261 gagccaagag ctatttgatg aaattagaat gacctacatc aaagagctag gaaaagccat
     4321 tgtcaagagg gaaggaaact ccagccagaa ctggcagcgg ttttatcaac tgacaaaact
     4381 cttggattct atgcatgaag tggttgaaaa tctccttaac tattgcttcc aaacattttt
     4441 ggataagacc atgagtactg aattccccga gatgttagct gaaatcatca ccaatcagat
     4501 accaaaatat tcaaatggaa atatcaaaaa acttctgttt catcaaaagg ggtctggttg
     4561 gtctcatccg caattcgaga agtgagcact agaggaattc cgcccctctc cccccccccc
     4621 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     4681 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     4741 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     4801 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     4861 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     4921 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     4981 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     5041 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     5101 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     5161 aaaaacacga tgataagctt gccacaaccc gtaccaaaga tggatagatc cggaaagcct
     5221 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     5281 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     5341 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     5401 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     5461 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     5521 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     5581 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     5641 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     5701 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     5761 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     5821 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     5881 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     5941 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     6001 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     6061 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     6121 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     6181 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagagtaga
     6241 tgccgaccga acaagagctg atttcgagaa cgcctcagcc agcaactcgc gcgagcctag
     6301 caaggcaaat gcgagagaac ggccttacgc ttggtggcac agttctcgtc cacagttcgc
     6361 taagctcgct cggctgggtc gcgggagggc cggtcgcagt gattcaggcc cttctggatt
     6421 gtgttggtcc ccagggcacg attgtcatgc ccacgcactc gggtgatctg actgatcccg
     6481 cagattggag atcgccgccc gtgcctgccg attgggtgca gatctctaga taactgatca
     6541 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     6601 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     6661 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6721 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgctcg agtgcatgga
     6781 tcctttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata aataaataaa
     6841 cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt
     6901 aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac acatgcgtca
     6961 attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct agggttaaag
     7021 agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
     7081 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7141 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7201 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7261 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8521 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8821 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     9241 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     9301 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     9361 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     9421 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     9481 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     9541 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     9601 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     9661 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     9721 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     9781 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     9841 gtgaattgta atacgactca ctatagggcg aattgggtac cttaacccta gaaagatagt
     9901 ctgcgtaaaa ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa
     9961 tccgtcgctg tgcatttagg acatctcagt cgccgcttgg agctcccgtg aggcgtgctt
    10021 gtcaatgcgg taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc
    10081 atgattatct tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc
    10141 atgttctact tacgtgataa cttattatat atatattttc ttgttataga tatcgtcgac
    10201 attgattatt gactagctag ttattaatag taatcaatta cggggtcatt agttcatagc
    10261 ccatatatgg agttccg
//
