LOCUS       sequence-326611-       19883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and 
            Lin28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19883)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 19883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Mar 26."
COMMENT     Sequence Label: pEP4 E02S CK2M EN2L
FEATURES             Location/Qualifiers
     source          1..19883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..1175
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          244..1166
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    2385..2958
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2408..2960
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    3977..4111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        4621..5000
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5001..5204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5155..5175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    6716..7289
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6739..7291
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             7351..8670
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     primer_bind     complement(8727..8744)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8733..8957
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        9178..10340
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9409..10331
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    11384..11957
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    11407..11959
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    12652..12786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(15540..17465)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGXGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGXGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGXGXGGXGGRGRG
                     GSGGRGRGGSGGRGRGGSGGRRGRGRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(17904..17922)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        17990..18094
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(18095..18163,18164..18955)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(18313..18332)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      19126..19714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     19615..19634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagcttcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
       61 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      121 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      181 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      241 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
      301 gtgccttgaa ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca
      361 ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      421 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      481 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      541 atagtcttgt aaatgcgggc caggatctgc acactggtat ttcggttttt ggggccgcgg
      601 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
      661 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
      721 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
      781 cgtgagcgga aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc
      841 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct
      901 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
      961 tctggagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1021 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1081 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1141 ttcaaagttt ttttcttcca tttcaggtgt cgtgaacacg tggtcgcggc caattctgca
     1201 gtcgagctca agcttcgaac tagtgattat ggcgggacac ctggcttcgg atttcgcctt
     1261 ctcgccccct ccaggtggtg gaggtgatgg gccagggggg ccggagccgg gctgggttga
     1321 tcctcggacc tggctaagct tccaaggccc tcctggaggg ccaggaatcg ggccgggggt
     1381 tgggccaggc tctgaggtgt gggggattcc cccatgcccc ccgccgtatg agttctgtgg
     1441 ggggatggcg tactgtgggc cccaggttgg agtggggcta gtgccccaag gcggcttgga
     1501 gacctctcag cctgagggtg aagcaggagt cggggtggag agcaactccg atggggcctc
     1561 cccggagccc tgcaccgtca cccctggtgc cgtgaagctg gagaaggaga agctggagca
     1621 aaacccggag gagtcccagg acatcaaagc tctgcagaaa gaactcgagc aatttgccaa
     1681 gctcctgaag cagaagagga tcaccctggg atatacacag gccgatgtgg ggctcaccct
     1741 gggggttcta tttgggaagg tattcagcca aacgaccatc tgccgctttg aggctctgca
     1801 gcttagcttc aagaacatgt gtaagctgcg gcccttgctg cagaagtggg tggaggaagc
     1861 tgacaacaat gaaaatcttc aggagatatg caaagcagaa accctcgtgc aggcccgaaa
     1921 gagaaagcga accagtatcg agaaccgagt gagaggcaac ctggagaatt tgttcctgca
     1981 gtgcccgaaa cccacactgc agcagatcag ccacatcgcc cagcagcttg ggctcgagaa
     2041 ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag ggcaagcgat caagcagcga
     2101 ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct ttctcagggg gaccagtgtc
     2161 ctttcctctg gccccagggc cccattttgg taccccaggc tatgggagcc ctcacttcac
     2221 tgcactgtac tcctcggtcc ctttccctga gggggaagcc tttccccctg tctccgtcac
     2281 cactctgggc tctcccatgc attcaaactg aggtgcctgc cctctagttt acgcgtgggg
     2341 gcggccggcc gctttctcga ggccatcgat gtttaaacga tccgcccctc tccctccccc
     2401 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     2461 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     2521 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     2581 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     2641 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     2701 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     2761 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     2821 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt
     2881 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     2941 tgaaaaacac gatgataata tggccacacc gcgaattaat tcgattccgc atgtacaaca
     3001 tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc ggcggcggca
     3061 actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc gtcaagcggc
     3121 ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc caggagaacc
     3181 ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa cttttgtcgg
     3241 agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg cacatgaagg
     3301 agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg aagaaggata
     3361 agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg agcggggtcg
     3421 gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac gcgcacatga
     3481 acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac ccgcagcacc
     3541 cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac gacgtgagcg
     3601 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca
     3661 gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg ggttcggtgg
     3721 tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac tccagggcgc
     3781 cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc gccgaggtgc
     3841 cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc ggcccggtgc
     3901 ccggcacggc cattaacggc acactgcccc tctcacacat gtgagggccg gaaatcacta
     3961 gctagttcga aggatcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4021 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4081 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4141 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     4201 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     4261 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4321 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gaggtcgacc
     4381 aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc cattgctgca
     4441 ggcgcagaac tggtaggtat ggaagatcga tctatacatt gaatcaatat tggcaattag
     4501 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     4561 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
     4621 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4681 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     4741 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     4801 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     4861 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     4921 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     4981 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     5041 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     5101 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     5161 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
     5221 agatccgcta ggatctcgag ctcaagcttc gaattcgcga tcgccatggc tgtcagcgac
     5281 gcgctgctcc catctttctc cacgttcgcg tctggcccgg cgggaaggga gaagacactg
     5341 cgtcaagcag gtgccccgaa taaccgctgg cgggaggagc tctcccacat gaagcgactt
     5401 cccccagtgc ttcccggccg cccctatgac ctggcggcgg cgaccgtggc cacagacctg
     5461 gagagcggcg gagccggtgc ggcttgcggc ggtagcaacc tggcgcccct acctcggaga
     5521 gagaccgagg agttcaacga tctcctggac ctggacttta ttctctccaa ttcgctgacc
     5581 catcctccgg agtcagtggc cgccaccgtg tcctcgtcag cgtcagcctc ctcttcgtcg
     5641 tcgccgtcga gcagcggccc tgccagcgcg ccctccacct gcagcttcac ctatccgatc
     5701 cgggccggga acgacccggg cgtggcgccg ggcggcacgg gcggaggcct cctctatggc
     5761 agggagtccg ctccccctcc gacggctccc ttcaacctgg cggacatcaa cgacgtgagc
     5821 ccctcgggcg gcttcgtggc cgagctcctg cggccagaat tggacccggt gtacattccg
     5881 ccgcagcagc cgcagccgcc aggtggcggg ctgatgggca agttcgtgct gaaggcgtcg
     5941 ctgagcgccc ctggcagcga gtacggcagc ccgtcggtca tcagcgtcag caaaggcagc
     6001 cctgacggca gccacccggt ggtggtggcg ccctacaacg gcgggccgcc gcgcacgtgc
     6061 cccaagatca cgcaggaggc ggtctcttcg tgcacccact tgggcgctgg accccctctc
     6121 agcaatggcc accggccggc tgcacacgac ttccccctgg ggcggcagct ccccagcagg
     6181 actaccccga ccctgggtct tgaggaagtg ctgagcagca gggactgtca ccctgccctg
     6241 ccgcttcctc ccggcttcca tccccacccg gggcccaatt acccatcctt cctgcccgat
     6301 cagatgcagc cgcaagtccc gccgctccat taccaagagc tcatgccacc cggttcctgc
     6361 atgccagagg agcccaagcc aaagagggga agacgatcgt ggccccggaa aaggaccgcc
     6421 acccacactt gtgattacgc gggctgcggc aaaacctaca caaagagttc ccatctcaag
     6481 gcacacctgc gaacccacac aggtgagaaa ccttaccact gtgactggga cggctgtgga
     6541 tggaaattcg cccgctcaga tgaactgacc aggcactacc gtaaacacac ggggcaccgc
     6601 ccgttccagt gccaaaaatg cgaccgagca ttttccaggt cggaccacct cgccttacac
     6661 atgaagaggc atttttaaat cccagacagt ggatatgacc cgtttaaacg atccgcccct
     6721 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     6781 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     6841 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     6901 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     6961 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     7021 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     7081 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     7141 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtacacat
     7201 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     7261 tggttttcct ttgaaaaaca cgatgataat atggccacac cgcgatcgcg aattctgcag
     7321 tcgagctcaa gcttcgaatt cgcgatcgcc atgcccctca acgttagctt caccaacagg
     7381 aactatgacc tcgactacga ctcggtgcag ccgtatttct actgcgacga ggaggagaac
     7441 ttctaccagc agcagcagca gagcgagctg cagcccccgg cgcccagcga ggatatctgg
     7501 aagaaattcg agctgctgcc caccccgccc ctgtccccta gccgccgctc cgggctctgc
     7561 tcgccctcct acgttgcggt cacacccttc tcccttcggg gagacaacga cggcggtggc
     7621 gggagcttct ccacggccga ccagctggag atggtgaccg agctgctggg aggagacatg
     7681 gtgaaccaga gtttcatctg cgacccggac gacgagacct tcatcaaaaa catcatcatc
     7741 caggactgta tgtggagcgg cttctcggcc gccgccaagc tcgtctcaga gaagctggcc
     7801 tcctaccagg ctgcgcgcaa agacagcggc agcccgaacc ccgcccgcgg ccacagcgtc
     7861 tgctccacct ccagcttgta cctgcaggat ctgagcgccg ccgcctcaga gtgcatcgac
     7921 ccctcggtgg tcttccccta ccctctcaac gacagcagct cgcccaagtc ctgcgcctcg
     7981 caagactcca gcgccttctc tccgtcctcg gattctctgc tctcctcgac ggagtcctcc
     8041 ccgcagggca gccccgagcc cctggtgctc catgaggaga caccgcccac caccagcagc
     8101 gactctgagg aggaacaaga agatgaggaa gaaatcgatg ttgtttctgt ggaaaagagg
     8161 caggctcctg gcaaaaggtc agagtctgga tcaccttctg ctggaggcca cagcaaacct
     8221 cctcacagcc cactggtcct caagaggtgc cacgtctcca cacatcagca caactacgca
     8281 gcgcctccct ccactcggaa ggactatcct gctgccaaga gggtcaagtt ggacagtgtc
     8341 agagtcctga gacagatcag caacaaccga aaatgcacca gccccaggtc ctcggacacc
     8401 gaggagaatg tcaagaggcg aacacacaac gtcttggagc gccagaggag gaacgagcta
     8461 aaacggagct tttttgccct gcgtgaccag atcccggagt tggaaaacaa tgaaaaggcc
     8521 cccaaggtag ttatccttaa aaaagccaca gcatacatcc tgtccgtcca agcagaggag
     8581 caaaagctca tttctgaaga ggacttgttg cggaaacgac gagaacagtt gaaacacaaa
     8641 cttgaacagc tacggaactc ttgtgcgtaa gtttggccgc ccccacgcgt aaactagttc
     8701 gaaggatcct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     8761 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     8821 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     8881 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     8941 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tgggggcgcg cccctcgaga
     9001 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     9061 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     9121 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagc ttcgtgaggc
     9181 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     9241 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     9301 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     9361 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     9421 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     9481 tccacctggc tccagtacgt gattcttgat cccgagctgg agccaggggc gggccttgcg
     9541 ctttaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     9601 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     9661 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     9721 cgggccagga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     9781 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     9841 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     9901 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     9961 ggccgcttcc cggccctgct ccagggggct caaaatggag gacgcggcgc tcgggagagc
    10021 gggcgggtga gtcacccaca caaaggaaag gggcctttcc gtcctcagcc gtcgcttcat
    10081 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctgg agcttttgga
    10141 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
    10201 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
    10261 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
    10321 ttccatttca ggtgtcgtga acacgtggtc gcggccaatt ctgcagtcga gctcaagctt
    10381 cgaactaggg ccgcgactct agtgattcat gagtgtggat ccagcttgtc cccaaagctt
    10441 gccttgcttt gaagcatccg actgtaaaga atcttcacct atgcctgtga tttgtgggcc
    10501 tgaagaaaac tatccatcct tgcaaatgtc ttctgctgag atgcctcaca cggagactgt
    10561 ctctcctctt ccttcctcca tggatctgct tattcaggac agccctgatt cttccaccag
    10621 tcccaaaggc aaacaaccca cttctgcaga gaagagtgtc gcaaaaaagg aagacaaggt
    10681 cccggtcaag aaacagaaga ccagaactgt gttctcttcc acccagctgt gtgtactcaa
    10741 tgatagattt cagagacaga aatacctcag cctccagcag atgcaagaac tctccaacat
    10801 cctgaacctc agctacaaac aggtgaagac ctggttccag aaccagagaa tgaaatctaa
    10861 gaggtggcag aaaaacaact ggccgaagaa tagcaatggt gtgacgcaga aggcctcagc
    10921 acctacctac cccagccttt actcttccta ccaccaggga tgcctggtga acccgactgg
    10981 gaaccttcca atgtggagca accagacctg gaacaattca acctggagca accagaccca
    11041 gaacatccag tcctggagca accactcctg gaacactcag acctggtgca cccaatcctg
    11101 gaacaatcag gcctggaaca gtcccttcta taactgtgga gaggaatctc tgcagtcctg
    11161 catgcagttc cagccaaatt ctcctgccag tgacttggag gctgccttgg aagctgctgg
    11221 ggaaggcctt aatgtaatac agcagaccac taggtatttt agtactccac aaaccatgga
    11281 tttattccta aactactcca tgaacatgca acctgaagac gtgtgaagat gaatcgaatt
    11341 cccgcggccg ctttctcgag gccatcgatg tttaaacgat ccgcccctct ccctcccccc
    11401 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
    11461 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
    11521 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
    11581 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
    11641 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
    11701 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
    11761 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
    11821 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg
    11881 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
    11941 gaaaaacacg atgataatat ggccacaccg cgatcgcgaa ttcgattatc gccatgggct
    12001 ccgtgtccaa ccagcagttt gcaggtggct gcgccaaggc ggcagaagag gcgcccgagg
    12061 aggcgccgga ggacgcggcc cgggcggcgg acgagcctca gctgctgcac ggtgcgggca
    12121 tctgtaagtg gttcaacgtg cgcatggggt tcggcttcct gtccatgacc gcccgcgccg
    12181 gggtcgcgct cgacccccca gtggatgtct ttgtgcacca gagtaagctg cacatggaag
    12241 ggttccggag cttgaaggag ggtgaggcag tggagttcac ctttaagaag tcagccaagg
    12301 gtctggaatc catccgtgtc accggacctg gtggagtatt ctgtattggg agtgagaggc
    12361 ggccaaaagg aaagagcatg cagaagcgca gatcaaaagg agacaggtgc tacaactgtg
    12421 gaggtctaga tcatcatgcc aaggaatgca agctgccacc ccagcccaag aagtgccact
    12481 tctgccagag catcagccat atggtagcct catgtccgct gaaggcccag cagggcccta
    12541 gtgcacaggg aaagccaacc tactttcgag aggaagaaga agaaatccac agccctaccc
    12601 tgctcccgga ggcacagaat tgagtttaaa caatcactag ctagttcgaa ggatccagac
    12661 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
    12721 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
    12781 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
    12841 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tccggctgcc
    12901 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    12961 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    13021 ttggcgggtg tcggggcgca gccatgaggt cgatcgactc tagctagagg atcgatcccc
    13081 gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat
    13141 gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg
    13201 gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt
    13261 gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg
    13321 caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg
    13381 acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg
    13441 ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag
    13501 gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc
    13561 agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag
    13621 ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt
    13681 tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg
    13741 gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc
    13801 cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct
    13861 cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg
    13921 gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt
    13981 aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt
    14041 aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc
    14101 aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg
    14161 ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca
    14221 ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa
    14281 caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc
    14341 acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa
    14401 ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga
    14461 atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga
    14521 caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg
    14581 aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc
    14641 tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
    14701 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
    14761 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
    14821 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgcatata
    14881 cmgataccaa aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
    14941 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
    15001 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
    15061 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
    15121 tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga gtgctatcct
    15181 ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc ttttcctgct
    15241 ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat
    15301 tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct
    15361 gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga
    15421 caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct
    15481 ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat
    15541 cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca
    15601 tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca
    15661 tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca
    15721 aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg
    15781 aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt
    15841 ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct
    15901 cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa
    15961 gttccttcgt cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct
    16021 gcaatgttct caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct
    16081 ccttttttgc gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct
    16141 gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg
    16201 ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt
    16261 ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc
    16321 ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg tcctctaccc
    16381 cggcggcctc cactacctcc tcgaccccgg cctccactac ctcctcgacc ccggcctcca
    16441 ctrcctcctc gaccccggcc tccacykcct cctcswcccc sgcctccacc tcctgctcct
    16501 gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc
    16561 cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct
    16621 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct
    16681 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
    16741 gctcctgccc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct
    16801 cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgctcct
    16861 gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
    16921 cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctsc ccctcctgcc
    16981 cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
    17041 gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctsc tcctgcccct
    17101 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
    17161 cctcctgctc ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg
    17221 cagccaatgc aacttggacg tttttggggt ctccggacac catctctatg tcttggccct
    17281 gatcctgagc cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt
    17341 cctcgtccat ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt
    17401 ccagatgtgt ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca
    17461 gacatgattc acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata
    17521 cagggagtgc agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc
    17581 gctgtcagac agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc
    17641 accaattact cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc
    17701 ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc
    17761 tcgggacccc tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga
    17821 agaaaagctg ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag
    17881 aattaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
    17941 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    18001 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    18061 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    18121 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    18181 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    18241 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    18301 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
    18361 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    18421 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    18481 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    18541 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    18601 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
    18661 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    18721 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    18781 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    18841 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    18901 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    18961 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    19021 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    19081 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    19141 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    19201 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    19261 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    19321 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    19381 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    19441 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    19501 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    19561 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    19621 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    19681 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    19741 gttcctggcc ttttgctggc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga
    19801 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg
    19861 gaaggccatc cagcctcgcg tcg
//
