LOCUS       sequence-326613-        7948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTriEx based vector for protein expression in mammalian cells for 
            targets fused with N-terminal uv-GFP, Flag and His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Bruni R, Kloss B
  TITLE     High-throughput cloning and expression of integral membrane proteins
            in Escherichia coli.
  JOURNAL   Curr Protoc Protein Sci. 2013 Nov 5;74:29.6.1-29.6.34. doi: 
            10.1002/0471140864.ps2906s74.
  PUBMED    24510647
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Protein Sci."; date: "2013-11-5"; volume: "74:29.6.1-29.6.34. doi"; 
            pages: " 10.1002/0471140864.ps2906s74"
COMMENT     Sequence Label: pRB33-GFP
FEATURES             Location/Qualifiers
     source          1..7948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     181..1008
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             181..305
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(343..948)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        1140..1443
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1449..1726
                     /label=chicken beta-actin promoter
     primer_bind     2005..2027
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2089..2108
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     2150..2169
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2150..2168
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2169..2193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2209..2318
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             2339..2368
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             2372..2395
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2396..3106
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2423..2451)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3046..3066
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3110..3130
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(3160..3193)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3208..3653
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(3654..3740,3741..5075)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYKVPEFDSSTIKNISSAKGLDVWDSWPLQNTDGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     complement(5243..5262)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5308..5363
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5362..5381)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      5451..5498
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     5511..6216
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           5599..5971
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(5628..6216)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(6225..6258)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(6426..7013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6505..6524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7026..7817,7818..7886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7649..7668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg
     1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct
     1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta
     1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc
     1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
     2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
     2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
     2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatgggcca
     2341 ccatcatcat catcatcacc accatcactc tgattacaag gatgacgacg ataagagcaa
     2401 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     2461 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gctacatacg gaaagcttac
     2521 ccttaaattt atttgcacta ctggaaaact acctgttccc tggccaacac ttgtcactac
     2581 tttctcttat ggtgttcaat gcttttcccg ttatccggat catatgaaac ggcatgactt
     2641 tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt tcaaagatga
     2701 cgggaactac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat
     2761 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt ctcggacaca aacttgagta
     2821 caactataac tcacacaatg tatacatcac ggcagacaaa caaaagaatg gaatcaaagc
     2881 taacttcaaa attcgccaca acattgaaga tggatccgtt caactagcag accattatca
     2941 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtcgac
     3001 acaatctgcc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc ttcttgagtt
     3061 tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaaaccg agaacctgta
     3121 cttccaatcc ataagctagc ttctcctcct gaaagatcca taacttcgta tagcatacat
     3181 tatacgaagt tatgcggccg cgacgtccac atatacctgc cgttcactat tatttagtga
     3241 aatgagatat tatgatattt tctgaattgt gattaaaaag gcaactttat gcccatgcaa
     3301 cagaaactat aaaaaataca gagaatgaaa agaaacagat agatttttta gttctttagg
     3361 cccgtagtct gcaaatcctt ttatgatttt ctatcaaaca aaagaggaaa atagaccagt
     3421 tgcaatccaa acgagagtct aatagaatga ggtcgaaaag taaatcgcgc gggtttgtta
     3481 ctgataaagc aggcaagacc taaaatgtgt aaagggcaaa gtgtatactt tggcgtcacc
     3541 ccttacatat tttaggtctt tttttattgt gcgtaactaa cttgccatct tcaaacagga
     3601 gggctggaag aagcagaccg ctaacacagt acataaaaaa ggagacatga acgatgaaca
     3661 tcaaaaagtt tgcaaaacaa gcaacagtat taacctttac taccgcactg ctggcaggag
     3721 gcgcaactca agcgtttgcg aaagaaacga accaaaagcc atataaggaa acatacggca
     3781 tttcccatat tacacgccat gatatgctgc aaatccctga acagcaaaaa aatgaaaaat
     3841 ataaagttcc tgagttcgat tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg
     3901 acgtttggga cagctggcca ttacaaaaca ctgacggcac tgtcgcaaac tatcacggct
     3961 accacatcgt ctttgcatta gccggagatc ctaaaaatgc ggatgacaca tcgatttaca
     4021 tgttctatca aaaagtcggc gaaacttcta ttgacagctg gaaaaacgct ggccgcgtct
     4081 ttaaagacag cgacaaattc gatgcaaatg attctatcct aaaagaccaa acacaagaat
     4141 ggtcaggttc agccacattt acatctgacg gaaaaatccg tttattctac actgatttct
     4201 ccggtaaaca ttacggcaaa caaacactga caactgcaca agttaacgta tcagcatcag
     4261 acagctcttt gaacatcaac ggtgtagagg attataaatc aatctttgac ggtgacggaa
     4321 aaacgtatca aaatgtacag cagttcatcg atgaaggcaa ctacagctca ggcgacaacc
     4381 atacgctgag agatcctcac tacgtagaag ataaaggcca caaatactta gtatttgaag
     4441 caaacactgg aactgaagat ggctaccaag gcgaagaatc tttatttaac aaagcatact
     4501 atggcaaaag cacatcattc ttccgtcaag aaagtcaaaa acttctgcaa agcgataaaa
     4561 aacgcacggc tgagttagca aacggcgctc tcggtatgat tgagctaaac gatgattaca
     4621 cactgaaaaa agtgatgaaa ccgctgattg catctaacac agtaacagat gaaattgaac
     4681 gcgcgaacgt ctttaaaatg aacggcaaat ggtacctgtt cactgactcc cgcggatcaa
     4741 aaatgacgat tgacggcatt acgtctaacg atatttacat gcttggttat gtttctaatt
     4801 ctttaactgg cccatacaag ccgctgaaca aaactggcct tgtgttaaaa atggatcttg
     4861 atcctaacga tgtaaccttt acttactcac acttcgctgt acctcaagcg aaaggaaaca
     4921 atgtcgtgat tacaagctat atgacaaaca gaggattcta cgcagacaaa caatcaacgt
     4981 ttgcgcctag cttcctgctg aacatcaaag gcaagaaaac atctgttgtc aaagacagca
     5041 tccttgaaca aggacaatta acagttaaca aataaaaacg caaaagaaaa tgccgatatc
     5101 ctattggcat tgacgtcagg tggcactttt cgaggagttt actagtaagt aaaggtggat
     5161 acggcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     5221 tcacaaatac cactgagatc gatctttttc cctctgccaa aaattatggg gacatcatga
     5281 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     5341 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     5401 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgtaa ctagcataac
     5461 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaagcatgc ggaggaaatt
     5521 ctccttgaag tttccctggt gttcaaagta aaggagtttg caccagacgc acctctgttc
     5581 actggtccgg cgtattaaaa cacgatacat tgttattagt acatttatta agcgctagat
     5641 tctgtgcgtt gttgatttac agacaattgt tgtacgtatt ttaataattc attaaattta
     5701 taatctttag ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc tttatatctg
     5761 aatttaaata ttaaatcctc aatagatttg taaaataggt ttcgattagt ttcaaacaag
     5821 ggttgttttt ccgaaccgat ggctggacta tctaatggat tttcgctcaa cgccacaaaa
     5881 cttgccaaat cttgtagcag caatctagct ttgtcgatat tcgtttgtgt tttgttttgt
     5941 aataaaggtt cgacgtcgtt caaaatatta tgcgcttttg tatttctttc atcactgtcg
     6001 ttagtgtaca attgactcga cgtaaacacg ttaaatagag cttggacata tttaacatcg
     6061 ggcgtgttag ctttattagg ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt
     6121 gcttccgaag acgattttgc catagccaca cgacgcctat taattgtgtc ggctaacacg
     6181 tccgcgatca aatttgtagt tgagcttttt ggaattgcga tcgcataact tcgtatagca
     6241 tacattatac gaagttataa gctcggaacg ctgcgctcgg tcgttcggct gcggcgagcg
     6301 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6421 gcgtttttcc ataggctccg ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6481 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6541 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6601 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6661 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6721 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6781 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6841 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6901 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6961 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7021 ctttgttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7081 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7141 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7201 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7261 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7321 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7381 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7441 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7501 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7561 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7621 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7681 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7741 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7801 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7861 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7921 cagggttatt gtctcatgtc cgcgcgtt
//
