LOCUS       sequence-326662-        7120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV for glutamate reporter fused to TARP gamma-8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Hao Y, Toulme E, Konig B, Rosenmund C, Plested AJR
  TITLE     Targeted sensors for glutamatergic neurotransmission.
  JOURNAL   eLife. 2023 Jan 9;12:e84029. doi: 10.7554/eLife.84029.
  PUBMED    36622100
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2023-01-9"; volume: "12:e84029. doi"; pages: " 
            10.7554/eLife.84029"
COMMENT     Sequence Label: pAAV-syn-SnFR-gamma8-minWPRE
FEATURES             Location/Qualifiers
     source          1..7120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..448
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     sig_peptide     540..599
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     1568..1589
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1695..1716)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1956..1975)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2186..2204
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(3826..3846)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   4222..4362
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4222..4351
                     /label=AAV2 ITR
     rep_origin      4437..4892
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4524..4543)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4734..4755
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4909..4928)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5028..5050
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5088..5106)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5174..5278
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5279..5347,5348..6139)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5497..5516)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6310..6898
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6799..6818
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6960..7089
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
       61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      421 aggagtcgtg tcgtgcctga gagcgcagtc gctagcgttt aaacttaagc ttggtaccga
      481 actcggatcc actagtaacg gccgccagtg tgctggaatt cggcttgggg atatccacca
      541 tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt tccactggtg
      601 acagatctgc cgcaggcagc acgctggaca aaatcgccaa aaacggtgtg attgtcgtcg
      661 gtcaccgtga atcttcagtg cctttctctt attacgacaa tcagcaaaaa gtggtgggtt
      721 actcgcagga ttactccaac gccattgttg aagcagtgaa aaagaaactc aacaaaccgg
      781 acttgcaggt aaaactgatt ccgattacct cacaaaaccg tattccactg ctgcaaaacg
      841 gcactttcga ttttgaatgt ggttctacca ccaacaacgt cgaacgccaa aaacaggcgg
      901 ctttctctga cactattttc gtggtcggta cgcgcctgtt gaccaaaaag ggtggcgata
      961 tcaaagattt tgccaacctg aaagacaaag ccgtagtcgt cacttccggc actacctctg
     1021 aagttttgct caacaaactg aatgaagagc aaaaaatgaa tatgcgcatc atcagcgcca
     1081 aagatcacgg tgactctttc cgcaccctgg aaagcggtcg tgccgttgcc tttatgatgg
     1141 atgacgtgct gctggccggt gaacgtgcga aagcgaagaa accagacaac tgggaaatcg
     1201 tcggcaagcc gcagtctcag gaggcctacg gttgtatgtt gcgtaaagat gatccgcagt
     1261 tcaaaaagct gatggatgac accatcgctc aggtgcagac ctccggtgaa gcggaaaaat
     1321 ggtttgataa gtggttcaaa aatccaattc tggtgagcca caacgtctat atcatggccg
     1381 acaagcagag gaacggcatc aaggcgaact tcaagatccg ccacaacatc gaggacggcg
     1441 gcgtgcagct cgcctaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1501 tgcccgacaa ccactacctg agcacccagt ccaaactttc gaaagacccc aacgagaagc
     1561 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1621 agctgtacaa gggcggtacc ggagggagca tggtgagcaa gggcgaggag ctgttcaccg
     1681 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1741 ccggcgaggg tgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1801 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1861 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1921 aaggctacat ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1981 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     2041 tcaaggagga cggcaacatc ctggggcata agctggagta caactttaac aaccccctga
     2101 acatgaattt cgaactgtca gacgaaatga aagcactgtt caaagaaccg aatgacaagg
     2161 cactgaaagg tgggcgcgcc cgggcagatg tgtataagag acaggaaaag aaaaaagcag
     2221 gactgtttga acaaatcacg aagactcatg ggacgatcat tggtgtcaca tcagggatcg
     2281 tcctggtcct tctcattatt tctattttag tacaagtgaa acagccccgg aaaaaggtca
     2341 tggcttgcaa aactgcattt aataaaactg ggttccagga agtatttgat cctcctcatt
     2401 atgaactatt ttcactaaga gagaaagaga tttctgcaga cgccgggtcc gagtcattga
     2461 aacgctggaa tgaagagagg gggttgtggt gcgaaaaggg cgttcaggta ctactgacca
     2521 ccataggcgc ctttgcagct tttggcctca tgaccatcgc catcagcact gactactggc
     2581 tctacacaag agctctcatc tgcaacacca ccaacctcac agcaggtgat gatggaccac
     2641 cccatcgtgg gggcagtggc tcctcagaga agaaggaccc tgggggcctc acacattcag
     2701 gcctctggcg gatatgctgc ctggaagggt tgaaaagagg tgtctgcgtg aagatcaacc
     2761 acttcccgga ggacacggac tacgaccacg acagcgcgga gtacctgctc cgagtagtcc
     2821 gggcctccag catctttcct atcctgagcg ccatcctgct gctgctcggg ggcgtgtgcg
     2881 tagctgcctc tcgcgtctac aaatccaaaa ggaacatcat cctgggcgca gggatcctgt
     2941 tcgtggcagc aggcctgagc aacatcatcg gggtgattgt gtacatatcg gccaacgccg
     3001 gcgagcctgg ccccaagagg gacgaggaga agaaaaacca ctactcgtac ggctggtcct
     3061 tctacttcgg cgggctgtcc ttcatcctgg ctgaggtgat cggagtactg gccgtcaaca
     3121 tctacatcga gcgcagccgc gaggcacact gccaatcacg ctcggacctg ctcaaggccg
     3181 gcggcggcgc gggcggcagt ggcgggagcg gcccctcggc catcctccgt ctgcccagtt
     3241 accgcttccg ctaccgccgc cgctcccgct ccagctcccg aggctccagc gaggcgtcgc
     3301 catcccggga tgcgtctccc ggcggccccg ggggcccggg cttcgcctcc acggacatct
     3361 ccatgtacac gctcagccgc gacccgtcca agggcagcgt ggctgcgggg ctggcgagcg
     3421 ccggtggcgg cggcagcggt gccggcgtgg gtgcctacgg cggggcggcc ggggcggcgg
     3481 ggggcggcgg ggcgggctcg gagcgggacc gcgggagctc ggcgggtttt ctcacgctgc
     3541 acaacgcctt ccccaaggaa gcggcgtccg gcgtcacggt cacagtcacc ggaccgcccg
     3601 ctgcacccgc gcccgcgccc gcgccgcccg ctcctgcagc gcccgcgccc gggaccctgt
     3661 ccaaagaggc cgcggcgtcc aacaccaaca cgctcaacag gaaaaccacg cccgtgtagg
     3721 aattctgcag atatccagca cagtggcggc cgctcgagtc tagactcgag ataatcaacc
     3781 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3841 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3901 cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc
     3961 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4021 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa
     4081 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     4141 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca
     4201 cggtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     4261 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     4321 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     4381 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     4441 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4501 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4561 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4621 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     4681 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4741 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
     4801 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4861 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     4921 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     4981 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5041 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5101 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5161 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5221 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5281 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5341 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5401 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5461 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5521 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5581 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5641 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5701 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5761 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5821 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5881 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5941 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6001 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6061 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6121 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6181 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6241 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6301 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6361 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6421 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6481 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6541 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6601 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6661 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6721 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6781 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6841 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6901 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtc
     6961 ctgcaggcag ctgcgcgctc gctcgctcac tgaggccgcc cgggcgtcgg gcgacctttg
     7021 gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta
     7081 ggggttcctg cggccgcacg cgtagagggc cctgcgtatg
//
