LOCUS       sequence-326664-       10697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for ER calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   de Juan-Sanz J, Holt GT, Schreiter ER, de Juan F, Kim DS, Ryan TA
  TITLE     Axonal Endoplasmic Reticulum Ca2+ Content Controls Release 
            Probability in CNS Nerve Terminals.
  JOURNAL   Neuron. 2017 Jan 30. pii: S0896-6273(17)30034-X. doi: 
            10.1016/j.neuron.2017.01.010.
  PUBMED    28162809
REFERENCE   2  (bases 1 to 10697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2017 Jan 30. pii: S0896-6273(17)30034-X. doi: 
            10.1016/j.neuron.2017.01.010."
COMMENT     Sequence Label: ER-GCaMP6-150
FEATURES             Location/Qualifiers
     source          1..10697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..43)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     270..290
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(299..727)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(409..430)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     621..640
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    773..997
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     986..1003
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             1029..1209
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1714..1730
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1715..1731
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1734..2322
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1840..1851
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2249..2269
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     2943..2962
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(3563..3619)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             complement(join(3647..3661,3662..3670))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(3710..3727)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      3799..3808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3821..5109)
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     misc_feature    5154..5271
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5569..5613)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5798..6031
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6524..6649
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6696..6876
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6894..7092)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6919..6939)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7094..7473
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7645..7664
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7739..7843
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7844..7912,7913..8704)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8062..8081)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8875..9463
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9364..9383
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9617..9634
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9751..9772
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9787..9792,9793..9810,9811..9817)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9825..9841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9830..9852
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9849..9865
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9849..9865
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9914..10035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9925..9944)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9979..9998
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(10165..10539)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(10558..10605)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(10653..10697,1..285))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10667..10697,1..105)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
       61 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
      121 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
      181 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
      241 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagaattaa
      301 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
      361 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
      421 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
      481 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
      541 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
      601 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
      661 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
      721 ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag
      781 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
      841 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
      901 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
      961 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa
     1021 acgggccctg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     1081 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     1141 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     1201 gagagaccca gtacagtccg gatgcagctc tcgggccatg tgatgaaatg ctaggcggct
     1261 gtcaaacctc cactctaata cttctctctc cgggtcatcc atcccatgca ggctcacagg
     1321 gtgtaacaag cgggtgttct ctccttcatt ggcttcttct accttctctt gctcaactgg
     1381 tactagcttg tagcaccatc caaaggtcag tggatatctg atccctggcc ctggtgtgta
     1441 gttctgccaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg
     1501 tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag
     1561 atctacagct gccttgtaag tcattggtct taaaggtacc tgaggtgtga ctggaaaacc
     1621 cacctcctcc tcctcttgtg cttctagcca ggcacaatca gcattggtag ctgctgtatt
     1681 gctacttgtg attgctccat gtttttctag gtctcgaggt cgacggtatc gatgcgggga
     1741 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg
     1801 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
     1861 gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga
     1921 cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
     1981 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
     2041 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
     2101 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
     2161 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
     2221 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
     2281 accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttatcgataa gcttgatatc
     2341 gaattctcga tcacagctca tccttgcctc cgccagcgct tccgcccttg gcggtcatca
     2401 tctgcacgaa ctcttcgtag ttcacttggc catccccgtc gatgtcggcc tctctgatca
     2461 tctcgtccac ctcttcgtct gtcagcttct cgcccaggtt ggtcatcacg tgtctcagct
     2521 cggcggcaga gatgtagccg ttgccatcct tgtcgaacac gccgaaggcc tcgcggatct
     2581 cttcctctgt gtctctgtac ttcatcttcc gggccatcat ggtcaggaac tcagggaagt
     2641 cgatagtgcc gtccccgcca gcgtccacct cgttgatcat gtcctgcagc tcggcctcgg
     2701 tagggttctg gcccaggctt ctcatcacgg tgcccagttc tttggtggtg attgttccgt
     2761 cgccgccctt atcgaacagg ctgaactctt ctttgaactc ggcgatctgt tcctcggtca
     2821 gctggtcggg caggttgtac tccagcttgt ggcccaggat gttgccgtcc tctttgaagt
     2881 cgatgccctt cagctcgatt ctgttcacga gggtgtcgcc ctcgaacttc acttcggccc
     2941 tggtcttgta gttgccgtcg tccttaaaga agatggttct ttcctggatg tagccctcgg
     3001 gcatggcgct cttgaagaag tcgtgctgct tcatatggtc ggggtatctg ctgaagcact
     3061 gcacgccata ggtcagtgtg gtcacgaggg taggccaagg cacgggcagc ttgccggtgg
     3121 tgcagatgaa cttcagggtc agctttccgt atgtggcgtc gccttcgccc tcgccggaca
     3181 cgctgaactt gtggccgttc acgtcgccat ccagttccac caggatgggc accacgccgg
     3241 tgaacagttc ctcgcccttg gacaccatgg agccgcctgt gccgcccttg tacagctcgt
     3301 ccatgcccag ggtgatgcca gcggcggtca caaattccag cagcaccatg tggtccctct
     3361 tctcgttggg gtccttgctc agcttgctct gcacgctcag gtagtggttg tcaggcagca
     3421 gcacagggcc gtcgccgata ggggtgttct gctggtagtg gtaggccagc tgcacgccgc
     3481 cgtcctcgat gttgtgccgg atgtggaagt tagccttgat gccgttcttc tgcttgtcgg
     3541 ccttgatgta cacgttttcc aggctagaca gtctgccgat ggcccgcacg gcgtggcctg
     3601 tcttgttcca cttccgcctg ctgctgtcca ccattgtggc caggtccttg tcatcgtcgt
     3661 cgtacagatc tctgcccatc tgctggccgc ctgtcatgct ggccatgccg tggtgatggt
     3721 ggtggtgaga gcccattccg ccaccggtag ccacagccag tcccagcagg cccagcagca
     3781 gaggcacaga cagcagtccc atggtggcgg cggatccccc gctgccccca gaactagggg
     3841 ccactcgcct gcccgtgctc ctgagtgcaa acggagaacc ggcttgactg acgagcttgg
     3901 ggcttctgag cagggcactg tggctgctca cagcctcgcg agccttcgtc agcatccagg
     3961 tccccatggc aaccacctcc gaaacgccct gttcccgttg ccccggtaac tgcctcccca
     4021 acacctgcct gcctttcact ttaaaacctg ctcctctttg ccctggcctt atatacacaa
     4081 tactgcagga ctgtgtgggc ccaggagcaa gtggaccctg ttcccccagg gtgatgtact
     4141 gagggggttg gagaagggga tgcgaacaaa cttagtccac aagtgatcat cgctcttcct
     4201 acctccccca cctccaacta tagggccctt ttaacagacg ggatgggatt aagtggggca
     4261 aagaggcagt tgctatggta acggctagtg ggtgccacat tctgggatgg tccttgagaa
     4321 agacaggatg tagttaccct ggcaacttca tctcctctga agagacaggt attgccccgt
     4381 tgccctggca acagctgggt tctaaggttt acaggggaag ctaattcatc tctccttccc
     4441 ttcccccttg tagtctctgt aatggtttct ttgcttcctc tgggaattgg tctggttgac
     4501 ttggcctctg tctctgtcct tgtttctttc cagctctccc tgctttttag ccattgtgca
     4561 tatcacaccg ccaccttagc gtgagaagaa gtaccaaaca gacccagaat gctgacggaa
     4621 tgactctgtc tgctttctca cccctatctg tcaccactga gcagcagggc ccctcctgag
     4681 gcctctgcca cagctctccc cacccatcct cccagaggga ccatgaagat ttggtctcct
     4741 ggggctcctg tcccagtttc tgcttgtgct tcccgtcaag gtaggactgg agagaggcaa
     4801 ctataatgag gtgaacgtgg ctggactcac ccagggctgg gcgggtggtg tagatgtacc
     4861 ctgccctgga ctcttctttc ctaaactcat tggtcatctg ccctaatgag ttcactcctt
     4921 ctgtatatcg tacttgcatg cccccaaggc atggaattta agattagtaa tctgagttca
     4981 agttctagct ctgacatttc tgatgtgacc ctgggcaggt cactgaggaa gctctctgca
     5041 cccccatctc attttctaga aaatcagaag aagaacccca tggagatgaa gtgacctaag
     5101 gccataatgt taattaacca aactggatct ctgctgtccc tgtaataaac ccgaaaattt
     5161 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
     5221 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg
     5281 aatactgcca tttgtctgca gaattggcgc acgcagtgcc gatccgttca ctaatcgaat
     5341 ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc
     5401 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     5461 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     5521 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     5581 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     5641 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     5701 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     5761 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     5821 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     5881 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     5941 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     6001 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     6061 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     6121 tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag
     6181 cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag
     6241 cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat
     6301 tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta
     6361 tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct
     6421 ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc
     6481 gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat
     6541 ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct
     6601 cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt
     6661 tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta
     6721 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     6781 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     6841 ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaac gcgctgctta
     6901 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     6961 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     7021 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     7081 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     7141 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     7201 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     7261 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     7321 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     7381 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     7441 attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgc
     7501 gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt
     7561 gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag
     7621 ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     7681 ggggatcggg agatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg
     7741 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7801 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7861 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7921 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7981 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8041 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8101 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8161 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8221 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8281 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8341 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8401 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8461 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8521 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8581 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8641 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8701 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8761 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8821 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8881 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8941 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9001 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9061 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9121 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9181 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9241 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9301 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9361 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9421 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9481 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9541 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9601 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9661 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     9721 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     9781 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     9841 atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg
     9901 tatacagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     9961 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
    10021 tgcaataaac aagttggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
    10081 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
    10141 ggaatcgaaa tctcgtagca cgtgtcagtc ctgctcctcg gccacgaagt gcacgcagtt
    10201 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
    10261 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
    10321 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
    10381 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
    10441 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
    10501 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtttagttcc tcaccttgtc
    10561 gtattatact atgccgatat actatgccga tgattaattg tcaacacgtg ctgatcagat
    10621 ccgaaaatgg atatacaagc tcccgggagc tttttgcaaa agcctaggcc tccaaaaaag
    10681 cctcctcact acttctg
//
