LOCUS       sequence-326680-        8826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full length SRGAP2 with a C-termonal EGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Guerrier S, Coutinho-Budd J, Sassa T, Gresset A, Jordan NV, Chen K, 
            Jin WL, Frost A, Polleux F
  TITLE     The F-BAR domain of srGAP2 induces membrane protrusions required for
            neuronal migration and morphogenesis.
  JOURNAL   Cell. 2009 Sep 4;138(5):990-1004. doi: 10.1016/j.cell.2009.06.047.
  PUBMED    19737524
REFERENCE   2  (bases 1 to 8826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.06"; journalName: "Cell"; date: "2009-09-4- 4";
            volume: "138"; issue: "5"; pages: "990-1004"
COMMENT     Sequence Label: pCAG-SRGAP2A-EGFP
FEATURES             Location/Qualifiers
     source          1..8826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(122..141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(800..1591,1592..1660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1423..1442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1661..1765)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1796..2175
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2177..2454
                     /label=chicken beta-actin promoter
     intron          2455..3467
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3391..3413
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3475..3494
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      6793..6802
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6799..6801,6802..6804,6805..7518)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6799..6801,6802..6804,6805..7515)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6844..6865)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7105..7124)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7452..7473
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7610..7629)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7675..7730
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7729..7748)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8121..8137)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8121..8137)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8134..8156)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8145..8161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8169..8175,8176..8193,8194..8199))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8214..8235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8294..8489
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8340..8475
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8495..8629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8532..8551)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8586..8605
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8697..8714)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 agacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      781 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      841 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
      901 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
      961 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1021 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1081 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1141 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1201 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1261 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1321 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1381 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1441 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1501 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1561 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1621 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1681 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1741 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat
     1801 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1861 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1921 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1981 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2041 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2101 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2161 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     2221 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     2281 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2341 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2401 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2461 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     2521 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     2581 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     2641 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     2701 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     2761 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     2821 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     2881 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     2941 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     3001 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3061 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     3121 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     3181 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     3241 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     3301 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     3361 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     3421 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3481 tgctggttat tgtgctgtct catcattttg gcaaagaatt gctcgagctc aagctaattc
     3541 atgacgtctc cagccaaatt caaaaaggat aaggagatca tagcagagta cgatactcag
     3601 gtcaaagaga tccgtgctca gctcacagag cagatgaaat gcctggacca gcagtgtgag
     3661 cttcgggtgc aactgttgca ggacctccag gacttcttcc gaaagaaggc agagattgag
     3721 atggactact cccgcaacct ggagaagctg gcagaacgct tcctggccaa gacacgcagc
     3781 accaaggacc agcaattcaa gaaggatcag aatgttctct ctccagtcaa ctgctggaat
     3841 ctcctcttaa accaggtgaa gcgggaaagc agggaccata ccaccctgag tgacatctac
     3901 ctgaataata tcattcctcg atttgtacaa gtcagcgagg actcaggaag actctttaaa
     3961 aagagtaaag aagtcggcca gcagctccaa gatgatttga tgaaggtcct gaacgagctc
     4021 tactcggtga tgaagacata tcacatgtac aatgccgaca gcatcagtgc tcagagcaaa
     4081 ctaaaggagg cggagaagca ggaggagaag caaattggta aatcggtaaa gcaggaggac
     4141 cggcagaccc cacgctcccc tgactccacg gccaacgttc gcattgagga gaaacatgtc
     4201 cggaggagct cagtgaagaa gattgagaag atgaaggaga agcgtcaagc caagtacacg
     4261 gagaataagc tgaaggccat caaagcccgg aatgagtact tgctggcttt ggaggcaacc
     4321 aatgcatctg tcttcaagta ctacatccat gacctatctg accttattga tcagtgttgt
     4381 gacttaggct accatgcaag tctgaaccgg gctctacgca ccttcctctc tgctgagtta
     4441 aacctggaac agtcgaagca tgagggtctg gatgccatcg agaatgcagt agaaaacctg
     4501 gatgccacca gtgacaagca gcgcctcatg gagatgtaca acaacgtctt ctgcccccct
     4561 atgaagtttg agtttcagcc ccacatgggg gatatggctt cccagctctg tgcccagcag
     4621 cctgtccaga gtgagctggt acagagatgc caacaactgc agtctcgctt atccactcta
     4681 aagattgaaa acgaagaggt aaagaagaca atggaggcca ccctgcaaac catccaggac
     4741 attgtgactg tcgaggactt tgatgtgtct gactgcttcc agtacagcaa ctccatggag
     4801 tccgtcaagt ccacggtctc tgaaaccttc atgagcaagc ccagcattgc taagaggaga
     4861 gccaaccagc aagagacaga gcagttttat ttcacaaaaa tgaaagagta cctggagggc
     4921 aggaacctca tcaccaagtt acaagccaag catgaccttc tgcagaaaac cctgggagaa
     4981 agtcagcgga cagattgcag tctagccagg cgcagctcaa ctgtgaggaa acaggactcc
     5041 agccaggcaa ttcctctggt ggtggaaagc tgtatccggt ttatcagcag acacggacta
     5101 cagcatgaag gaattttccg ggtgtcagga tcccaggtgg aagtgaatga catcaaaaat
     5161 gcctttgaga gaggagagga ccccctggct ggggaccaga acgaccatga catggattcc
     5221 atagctggtg tcctgaagct ttacttccgg gggctggaac accctctctt ccccaaggac
     5281 atctttcatg acctgatggc ctgcgtcaca atggacaacc tgcaggagag agctctgcac
     5341 atccggaaag tcctcctagt cctgcccaaa accactctga ttatcatgag atacctcttt
     5401 gccttcctca atcatttatc acagttcagt gaagagaaca tgatggaccc ctacaacctc
     5461 gccatctgct tcgggccctc gctaatgtca gtgccagagg gccacgacca ggtgtcctgc
     5521 caagcccacg tgaatgagct gatcaaaacc atcatcatcc agcatgagaa catcttccca
     5581 agccccaggg agctggaggg ccctgtctac agcagaggag gaagcatgga ggattactgt
     5641 gatagccctc atggagagac tacctcggtt gaagactcaa cccaggatgt gaccgcagag
     5701 caccacacga gcgatgacga atgtgagccc atcgaggcca ttgccaagtt tgactacgtg
     5761 ggccggacag cccgagagct gtcctttaag aagggagcat ccctgctgct ttaccagcgg
     5821 gcttccgacg actggtggga aggccggcac aatggcatcg acggactcat cccccatcag
     5881 tacatcgtgg tccaagacac cgaggacggt gtcgtggaga ggtccagccc caagtctgag
     5941 attgaggtca tttctgagcc acctgaagaa aaggtgacag ccagagcggg ggccagctgt
     6001 cccagtgggg gtcatgtagc cgatatttat cttgcaaaca tcaacaagca aaggaagcgt
     6061 ccagaatctg ggagcatccg gaaaactttt cggagtgaca gccatgggct gagcagttcc
     6121 ctgactgact cctcctcccc aggggtgggg gctagctgcc gcccatcctc ccagcccatc
     6181 atgagccaga gcctccccaa agaagggcca gataagtgtt ccatcagtgg gcacgggagc
     6241 ctcaactcca tcagccgcca ctcatccctg aagaatcggc tggatagtcc acagatccgg
     6301 aagactgcca cagcgggaag gtcaaaaagc ttcaataacc atcggcccat ggaccctgag
     6361 gtcattgctc aggatattga ggcaacaatg aactcggccc tgaatgagct acgggaacta
     6421 gaacggcaga gcagtgtcaa acacacccct gacgtggttc tggacacctt ggagcccctc
     6481 aaaacctccc cagtggtggc ccccacgtca gagccctcca gccctctgca cacccagctc
     6541 ctcaaggacc ccgagcccgc cttccagcgc agcgccagta ctgctgggga catcgcctgc
     6601 gccttccggc ctgtcaagtc tgtcaagatg gctgccccgg tcaaaccacc agccacacgg
     6661 cccaagccca ctgtcttccc caaaacaaat gccactagcc ctggtgtcaa ctcatcaact
     6721 tccccacagt ctactgacaa gtcttgtact gtcgctcgat cgacggtacc gcgggcccgg
     6781 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     6841 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     6901 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     6961 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     7021 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     7081 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     7141 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     7201 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     7261 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     7321 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     7381 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     7441 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     7501 gacgagctgt acaagtaaag cggccgcaat tcactcctca ggtgcaggct gcctatcaga
     7561 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
     7621 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     7681 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctatca ctcggaagga
     7741 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     7801 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     7861 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     7921 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     7981 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     8041 ttctcttatg gagatccctc gacctgcacc gtcgaccagc tggtcgacgg tgcaccgtcg
     8101 accagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8161 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8221 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8281 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8341 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8401 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8461 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg
     8521 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8581 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa
     8641 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8701 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8761 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8821 ggccag
//
