LOCUS       sequence-326682-        7030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal
            mRFP fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Guerrier S, Coutinho-Budd J, Sassa T, Gresset A, Jordan NV, Chen K, 
            Jin WL, Frost A, Polleux F
  TITLE     The F-BAR domain of srGAP2 induces membrane protrusions required for
            neuronal migration and morphogenesis.
  JOURNAL   Cell. 2009 Sep 4;138(5):990-1004. doi: 10.1016/j.cell.2009.06.047.
  PUBMED    19737524
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.06"; journalName: "Cell"; date: "2009-09-4- 4";
            volume: "138"; issue: "5"; pages: "990-1004"
COMMENT     Sequence Label: pCAG-SRGAP2A-FBAR-mRFP
FEATURES             Location/Qualifiers
     source          1..7030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(122..141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(800..1591,1592..1660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1423..1442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1661..1765)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1796..2175
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2177..2454
                     /label=chicken beta-actin promoter
     intron          2455..3467
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3391..3413
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3475..3494
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5046..5723
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             5046..5723
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(5176..5194)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5444..5463
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5638..5661
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5814..5833)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5879..5934
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5933..5952)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6325..6341)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6325..6341)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6338..6360)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6349..6365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6373..6379,6380..6397,6398..6403))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6418..6439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6498..6693
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6544..6679
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6699..6833
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6736..6755)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6790..6809
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6901..6918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 agacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      781 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      841 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
      901 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
      961 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1021 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1081 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1141 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1201 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1261 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1321 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1381 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1441 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1501 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1561 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1621 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1681 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1741 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat
     1801 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1861 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1921 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1981 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2041 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2101 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2161 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     2221 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     2281 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2341 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2401 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2461 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     2521 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     2581 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     2641 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     2701 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     2761 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     2821 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     2881 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     2941 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     3001 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3061 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     3121 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     3181 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     3241 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     3301 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     3361 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     3421 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3481 tgctggttat tgtgctgtct catcattttg gcaaagaatt gctcgaggcc accatgacgt
     3541 ctccagccaa attcaaaaag gataaggaga tcatcgcaga gtacgatact caggtcaaag
     3601 agattagggc tcagctcaca gagcagatga aatgcctaga tcagcaatgt gagctccggg
     3661 tgcagctgtt gcaggacctg caggacttct tccgcaagaa ggctgagatt gaaatggact
     3721 actctcgaaa cctggagaag ctagccgagc gctttctagc caagacacga agcaccaagg
     3781 accagcaatt taagaaggac cagaatgttc tttctccagt caactgctgg aacctcctct
     3841 taaaccaagt gaagcgggag agcagggatc ataccaccct gagtgacatc tacctgaata
     3901 atataattcc tcgatttgtt caagtcagcg aggactcagg aagactcttt aaaaagagta
     3961 aagaagttgg ccagcaactc caagatgatt tgatgaaagt cctgaacgag ctttactcgg
     4021 tcatgaagac atatcacatg tacaatgccg acagcatcag tgctcagagc aaactgaagg
     4081 aagcagagaa acaagaagag aagcaaattg gcaaatcagt aaagcaagag gaccggcaga
     4141 cccctcgctc ccctgactcc acagccaatg tccgcattga ggagaagcat gtccggagga
     4201 gctcagtgaa gaagattgag aagatgaagg agaagcgaca agccaagtac acagaaaata
     4261 agctgaaggc cattaaagcc cggaatgagt atttattggc tttggaggca accaatgcat
     4321 ctgtcttcaa gtactatatc catgacctgt ctgatattat tgatcaatgt tgtgacctag
     4381 gctaccatgc tagcctgaac cgggctctac gcactttcct atctgctgaa ttaaatctgg
     4441 aacagtcaaa acatgaaggt ctggatgcta ttgaaaatgc agtagaaaac ctagatgcca
     4501 ccagtgacaa gcaacggctc atggagatgt acaacaatgt tttttgcccc cctatgaaat
     4561 ttgaattcca gccccacatg ggagatatgg cctctcaact ctgtgcccag cagcctgtcc
     4621 agagtgagct ggtgcagaga tgccaacagc tgcagtctcg cttatccact ttgaagattg
     4681 agaatgaaga ggtgaaaaag acaatggagg ccaccctgca gaccattcaa gacattgtga
     4741 ctgttgagga ttttgatgta tctgactgct tccagtatag caattccatg gagtctgtca
     4801 aatcaactgt ctctgaaaca ttcatgagca agcccagcat tgctaagagg agagcgaacc
     4861 agcaagagac ggaacagttt tatttcacga aaatgaagga gtacttggag ggtaggaacc
     4921 tcatcacaaa gctacaagcc aagcatgacc tcctgcagaa aaccctggga gaaagtcagc
     4981 ggacagactg tagtcttgcc aggcgaagct caactgtgag gaaacaggat tccagcgccc
     5041 gggcaatggc ctcctccgag gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg
     5101 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     5161 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     5221 acatcctgtc ccctcagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca
     5281 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     5341 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     5401 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     5461 agaccatggg ctgggaggcc tccaccgagc ggatgtaccc cgaggacggc gccctgaagg
     5521 gcgagatcaa gatgaggctg aagctgaagg acggcggcca ctacgacgcc gaggtcaaga
     5581 ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc
     5641 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg
     5701 gccgccactc caccggcgcc taagcggccg caattcactc ctcaggtgca ggctgcctat
     5761 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
     5821 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     5881 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct atcactcgga
     5941 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     6001 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
     6061 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     6121 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     6181 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     6241 cctcttctct tatggagatc cctcgacctg caccgtcgac cagctggtcg acggtgcacc
     6301 gtcgaccagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6361 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6421 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6481 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6541 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6601 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     6661 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
     6721 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6781 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
     6841 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7021 aaaaggccag
//
