LOCUS       sequence-326726-        6518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yEVenus under the control of GAL1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Gligorovski V, Sadeghi A, Rahi SJ
  TITLE     Multidimensional characterization of inducible promoters and a 
            highly light-sensitive LOV-transcription factor.
  JOURNAL   Nat Commun. 2023 Jun 27;14(1):3810. doi: 10.1038/s41467-023-38959-8.
  PUBMED    37369667
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-06-27"; volume: "14(1):3810. doi"; pages: " 
            10.1038/s41467-023-38959-8"
COMMENT     Sequence Label: pVG49
FEATURES             Location/Qualifiers
     source          1..6518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..39
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        21..39
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        294..735
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    294..411
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     706..729
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             745..1458
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     terminator      2016..2203
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2071..2095)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2271..2287)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2272..2288)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2312..2331)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2313..2331)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2341..2358)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2341..2357)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2350..2372)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2498..2953
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2585..2604)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2795..2816
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3084..3887)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3888..4103)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(4127..4146)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4246..4268
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4306..4324)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4392..4496
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4497..4565,4566..5357)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4715..4734)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5528..6116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6017..6036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6270..6287
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6404..6425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6440..6445,6446..6463,6464..6470)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6478..6494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6483..6505
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6502..6518
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6502..6518
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac
       61 cgcggtggcg gccgccagct gaagcttcgt acgctgcagg tcgacggatc ctttggatgg
      121 acgcaaagaa gtttaataat catattacat ggcattacca ccatatacat atccatatct
      181 aatcttactt atatgttgtg gaaatgtaaa gagccccatt atcttagcct aaaaaaacct
      241 tctctttgga actttcagta atacgcttaa ctgctcattg ctatattgaa gtacggatta
      301 gaagccgccg agcgggcgac agccctccga cggaagactc tcctccgtgc gtcctcgtct
      361 tcaccggtcg cgttcctgaa acgcagatgt gcctcgcgcc gcactgctcc gaacaataaa
      421 gattctacaa tactagcttt tatggttatg aagaggaaaa attggcagta acctggcccc
      481 acaaaccttc aaattaacga atcaaattaa caaccatagg atgataatgc gattagtttt
      541 ttagccttat ttctggggta attaatcagc gaagcgatga tttttgatct attaacagat
      601 atataaatgg aaaagctgca taaccacttt aactaatact ttcaacattt tcagtttgta
      661 ttacttctta ttcaaatgtc ataaaagtat caacaaaaaa ttgttaatat acctctatac
      721 tttaacgtca aggagttaat taacatgtct aaaggtgaag aattattcac tggtgttgtc
      781 ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa
      841 ggtgaaggtg atgctactta cggtaaattg accttaaaat tgatttgtac tactggtaaa
      901 ttgccagttc catggccaac cttagtcact actttaggtt atggtttgca atgttttgct
      961 agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat
     1021 gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc
     1081 aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa
     1141 gatggtaaca ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc
     1201 actgctgaca aacaaaagaa tggtatcaaa gctaacttca aaattagaca caacattgaa
     1261 gatggtggtg ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca
     1321 gtcttgttac cagacaacca ttacttatcc tatcaatctg ccttatccaa agatccaaac
     1381 gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt
     1441 attgatgaat tgtacaaagc atccaacttg aacatttcga gaaagcttac catatcaacc
     1501 ccatcatgct ctttcgaaaa ttcaaatagc acatccattc cttcgcccgc ttcctcatct
     1561 caaagccaca ctccaatgag aaacatgagc tcactctctg ataacagcgt tttcagccgg
     1621 aatatggaac aatcatcacc aatcactcca agtatgtacc aatttggtca gcagcagtca
     1681 aacagtatat gtggtagcac cgttagtgtg aatagtctgg tgaatacaaa taacaaacaa
     1741 aggatctacg aacaaatcac gggtcctaac agcaataacg caaccaatga ttatattgat
     1801 ttgctaaacc taaatgagtc taacaaggaa aaccaaaatc ccgcaacggc gcattacctc
     1861 aatgggggcc cacccaagac aagcttcatt aaccatggaa tgttcccctc gccaactggg
     1921 accataaata gcggtaaatc tagcagtgcc tcatctttaa tttcttttgg tatgggcaat
     1981 acccaagtaa tatagggcgc gccacttcta aataagcgaa tttcttatga tttatgattt
     2041 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     2101 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     2161 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata
     2221 acagggtaat atagatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc
     2281 gacctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     2341 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     2401 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     2461 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     2521 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2581 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2641 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2701 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2761 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     2821 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     2881 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     2941 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3001 gcatagggta ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat
     3061 gcatttactt ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc
     3121 cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt
     3181 cctcttccaa caataataat gtcagatcct gtagagacca catcatccac ggttctatac
     3241 tgttgaccca atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa
     3301 tcgtaacctt catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct
     3361 ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc
     3421 ttgcatgaca attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc
     3481 gcctgcttca aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct
     3541 gcccattctg ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg
     3601 tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc
     3661 ggcttaactg tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa
     3721 caaattttgg gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc
     3781 aatgaagcac acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca
     3841 ggactaggat gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt
     3901 ttcctgcagg tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct
     3961 tcaacactac atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc
     4021 ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa
     4081 taaaaaaaaa atgatgaatt gaaaaggtgg tatggtgcac tctcagtaca atctgctctg
     4141 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4201 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4261 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     4321 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4381 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4441 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4501 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4561 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4621 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4681 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4741 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4801 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4861 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4921 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4981 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5041 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5101 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5161 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5221 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5281 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5341 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5401 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5461 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5641 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6301 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6361 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6421 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6481 tgagcggata acaatttcac acaggaaaca gctatgac
//
