LOCUS       sequence-326741-        3765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 SARS-CoV-1 S_nostop
FEATURES             Location/Qualifiers
     source          1..3765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3715..3726
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 atgtttattt tcttattatt tcttactctc actagtggta gtgaccttga ccggtgcacc
       61 acttttgatg atgttcaagc tcctaattac actcaacata cttcatctat gaggggggtt
      121 tactatcctg atgaaatttt tagatcagac actctttatt taactcagga tttatttctt
      181 ccattttatt ctaatgttac agggtttcat actattaatc atacgtttgg caaccctgtc
      241 atacctttta aggatggtat ttattttgct gccacagaga aatcaaatgt tgtccgtggt
      301 tgggtttttg gttctaccat gaacaacaag tcacagtcgg tgattattat taacaattct
      361 actaatgttg ttatacgagc atgtaacttt gaattgtgtg acaacccttt ctttgctgtt
      421 tctaaaccca tgggtacaca gacacatact atgatattcg ataatgcatt taattgcact
      481 ttcgagtaca tatctgatgc cttttcgctt gatgtttcag aaaagtcagg taattttaaa
      541 cacttacgag agtttgtgtt taaaaataaa gatgggtttc tctatgttta taagggctat
      601 caacctatag atgtagttcg tgatctacct tctggtttta acactttgaa acctattttt
      661 aagttgcctc ttggtattaa cattacaaat tttagagcca ttcttacagc cttttcacct
      721 gctcaagaca tttggggcac gtcagctgca gcctattttg ttggctattt aaagccaact
      781 acatttatgc tcaagtatga tgaaaatggt acaatcacag atgctgttga ttgttctcaa
      841 aatccacttg ctgaactcaa atgctctgtt aagagctttg agattgacaa aggaatttac
      901 cagacctcta atttcagggt tgttccctca ggagatgttg tgagattccc taatattaca
      961 aacttgtgtc cttttggaga ggtttttaat gctactaaat tcccttctgt ctatgcatgg
     1021 gagagaaaaa aaatttctaa ttgtgttgct gattactctg tgctctacaa ctcaacattt
     1081 ttttcaacct ttaagtgcta tggcgtttct gccactaagt tgaatgatct ttgcttctcc
     1141 aatgtctatg cagattcttt tgtagtcaag ggagatgatg taagacaaat agcgccagga
     1201 caaactggtg ttattgctga ttataattat aaattgccag atgatttcat gggttgtgtc
     1261 cttgcttgga atactaggaa cattgatgct acttcaactg gtaattataa ttataaatat
     1321 aggtatctta gacatggcaa gcttaggccc tttgagagag acatatctaa tgtgcctttc
     1381 tcccctgatg gcaaaccttg caccccacct gctcttaatt gttattggcc attaaatgat
     1441 tatggttttt acaccactac tggcattggc taccaacctt acagagttgt agtactttct
     1501 tttgaacttt taaatgcacc ggccacggtt tgtggaccaa aattatccac tgaccttatt
     1561 aagaaccagt gtgtcaattt taattttaat ggactcactg gtactggtgt gttaactcct
     1621 tcttcaaaga gatttcaacc atttcaacaa tttggccgtg atgtttctga tttcactgat
     1681 tccgttcgag atcctaaaac atctgaaata ttagacattt caccttgcgc ttttgggggt
     1741 gtaagtgtaa ttacacctgg aacaaatgct tcatctgaag ttgctgttct atatcaagat
     1801 gttaactgca ctgatgtttc tacagcaatt catgcagatc aactcacacc agcttggcgc
     1861 atatattcta ctggaaacaa tgtattccag actcaagcag gctgtcttat aggagctgag
     1921 catgtcgaca cttcttatga gtgcgacatt cctattggag ctggcatttg tgctagttac
     1981 catacagttt ctttattacg tagtactagc caaaaatcta ttgtggctta tactatgtct
     2041 ttaggtgctg atagttcaat tgcttactct aataacacca ttgctatacc tactaacttt
     2101 tcaattagca ttactacaga agtaatgcct gtttctatgg ctaaaacctc cgtagattgt
     2161 aatatgtaca tctgcggaga ttctactgaa tgtgctaatt tgcttctcca atatggtagc
     2221 ttttgcacac aactaaatcg tgcactctca ggtattgctg ctgaacagga tcgcaacaca
     2281 cgtgaagtgt tcgctcaagt caaacaaatg tacaaaaccc caactttgaa atattttggt
     2341 ggttttaatt tttcacaaat attacctgac cctctaaagc caactaagag gtcttttatt
     2401 gaggacttgc tctttaataa ggtgacactc gctgatgctg gcttcatgaa gcaatatggc
     2461 gaatgcctag gtgatattaa tgctagagat ctcatttgtg cgcagaagtt caatggactt
     2521 acagtgttgc cacctctgct cactgatgat atgattgctg cctacactgc tgctctagtt
     2581 agtggtactg ccactgctgg atggacattt ggtgctggcg ctgctcttca aatacctttt
     2641 gctatgcaaa tggcatatag gttcaatggc attggagtta cccaaaatgt tctctatgag
     2701 aaccaaaaac aaatcgccaa ccaatttaac aaggcgatta gtcaaattca agaatcactt
     2761 acaacaacat caactgcatt gggcaagctg caagacgttg ttaaccagaa tgctcaagca
     2821 ttaaacacac ttgttaaaca acttagctct aattttggtg caatttcaag tgtgctaaat
     2881 gatatccttt cgcgacttga taaagtcgag gcggaggtac aaattgacag gttaattaca
     2941 ggcagacttc aaagccttca aacctatgta acacaacaac taatcagggc tgctgaaatc
     3001 agggcttctg ctaatcttgc tgctactaaa atgtctgagt gtgttcttgg acaatcaaaa
     3061 agagttgact tttgtggaaa gggctaccac cttatgtcct tcccacaagc agccccgcat
     3121 ggtgttgtct tcctacatgt cacgtatgtg ccatcccagg agaggaactt caccacagcg
     3181 ccagcaattt gtcatgaagg caaagcatac ttccctcgtg aaggtgtttt tgtgtttaat
     3241 ggcacttctt ggtttattac acagaggaac ttcttttctc cacaaataat tactacagac
     3301 aatacatttg tctcaggaaa ttgtgatgtc gttattggca tcattaacaa cacagtttat
     3361 gatcctctgc aacctgagct tgactcattc aaagaagagc tggacaagta cttcaaaaat
     3421 catacatcac cagatgttga tcttggcgac atttcaggca ttaacgcttc tgtcgtcaac
     3481 attcaaaaag aaattgaccg cctcaatgag gtcgctaaaa atttaaatga atcactcatt
     3541 gaccttcaag aattgggaaa atatgagcaa tatattaaat ggccttggta tgtttggctc
     3601 ggcttcattg ctggactaat tgccatcgtc atggttacaa tcttgctttg ttgcatgact
     3661 agttgttgca gttgcctcaa gggtgcatgc tcttgtggtt cttgctgcaa gtttgatgag
     3721 gatgactctg agccagttct caagggtgtc aaattacatt acaca
//
