LOCUS       sequence-326828-        4062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 MERS-CoV S_nostop
FEATURES             Location/Qualifiers
     source          1..4062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3565..3576)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgatacact cagtgtttct actgatgttc ttgttaacac ctacagaaag ttacgttgat
       61 gtagggccag attctgttaa gtctgcttgt attgaggttg atatacaaca gactttcttt
      121 gataaaactt ggcctaggcc aattgatgtt tctaaggctg acggtattat ataccctcaa
      181 ggccgtacat attctaacat aactatcact tatcaaggtc tttttcccta tcagggagac
      241 catggtgata tgtatgttta ctctgcagga catgctacag gcacaactcc acaaaagttg
      301 tttgtagcta actattctca ggacgtcaaa cagtttgcta atgggtttgt cgtccgtata
      361 ggagcagctg ccaattccac tggcactgtt attattagcc catctaccag cgctactata
      421 cgaaaaattt accctgcttt tatgctgggt tcttcagttg gtaatttctc agatggtaaa
      481 atgggccgct tcttcaatca tactctagtt cttttgcccg atggatgtgg cactttactt
      541 agagcttttt attgtattct agagcctcgc tctggaaatc attgtcctgc tggcaattcc
      601 tatacttctt ttgccactta tcacactcct gcaacagatt gttctgatgg caattacaat
      661 cgtaatgcca gtctgaactc ttttaaggag tattttaatt tacgtaactg cacctttatg
      721 tacacttata acattaccga agatgagatt ttagagtggt ttggcattac acaaactgct
      781 caaggtgttc acctcttctc atctcggtat gttgatttgt acggcggcaa tatgtttcaa
      841 tttgccacct tgcctgttta tgatactatt aagtattatt ctatcattcc tcacagtatt
      901 cgttctatcc aaagtgatag aaaagcttgg gctgccttct acgtatataa acttcaaccg
      961 ttaactttcc tgttggattt ttctgttgat ggttatatac gcagagctat agactgtggt
     1021 tttaatgatt tgtcacaact ccactgctca tatgaatcct tcgatgttga atctggagtt
     1081 tattcagttt cgtctttcga agcaaaacct tctggctcag ttgtggaaca ggctgaaggt
     1141 gttgaatgtg atttttcacc tcttctgtct ggcacacctc ctcaggttta taatttcaag
     1201 cgtttggttt ttaccaattg caattataat cttaccaaat tgctttcact tttttctgtg
     1261 aatgatttta cttgtagtca aatatctcca gcagcaattg ctagcaactg ttattcttca
     1321 ctgattttgg attacttttc atacccactt agtatgaaat ccgatctcag tgttagttct
     1381 gctggtccaa tatcccagtt taattataaa cagtcctttt ctaatcccac atgtttgatt
     1441 ttagcgactg ttcctcataa ccttactact attactaagc ctcttaagta cagctatatt
     1501 aacaagtgct ctcgtcttct ttctgatgat cgtactgaag tacctcagtt agtgaacgct
     1561 aatcaatact caccctgtgt atccattgtc ccatccactg tgtgggaaga cggtgattat
     1621 tataggaaac aactatctcc acttgaaggt ggtggctggc ttgttgctag tggctcaact
     1681 gttgccatga ctgagcaatt acagatgggc tttggtatta cagttcaata tggtacagac
     1741 accaatagtg tttgccccaa gcttgaattt gctaatgaca caaaaattgc ctctcaatta
     1801 ggcaattgcg tggaatattc cctctatggt gtttcgggcc gtggtgtttt tcagaattgc
     1861 acagctgtag gtgttcgaca gcagcgcttt gtttatgatg cgtaccagaa tttagttggc
     1921 tattattctg atgatggcaa ctactactgt ttgcgtgctt gtgttagtgt tcctgtttct
     1981 gtcatctatg ataaagaaac taaaacccac gctactctat ttggtagtgt tgcatgtgaa
     2041 cacatttctt ctaccatgtc tcaatactcc cgttctacgc gatcaatgct taaacggcga
     2101 gattctacat atggccccct tcagacacct gttggttgtg tcctaggact tgttaattcc
     2161 tctttgttcg tagaggactg caagttgcct cttggtcaat ctctctgtgc tcttcctgac
     2221 acacctagta ctctcacacc tcgcagtgtg cgctctgttc caggtgaaat gcgcttggca
     2281 tccattgctt ttaatcatcc tattcaggtt gatcaactta atagtagtta ttttaaatta
     2341 agtataccca ctaatttttc ctttggtgtg actcaggagt acattcagac aaccattcag
     2401 aaagttactg ttgattgtaa acagtacgtt tgcaatggtt tccagaagtg tgagcaatta
     2461 ctgcgcgagt atggccagtt ttgttccaaa ataaaccagg ctctccatgg tgccaattta
     2521 cgccaggatg attctgtacg taatttgttt gcgagcgtga aaagctctca atcatctcct
     2581 atcataccag gttttggagg tgactttaat ttgacacttc tagaacctgt ttctatatct
     2641 actggcagtc gtagtgcacg tagtgctatt gaggatttgc tatttgacaa agtcactata
     2701 gctgatcctg gttatatgca aggttacgat gattgcatgc agcaaggtcc agcatcagct
     2761 cgtgatctta tttgtgctca atatgtggct ggttacaaag tattacctcc tcttatggat
     2821 gttaatatgg aagccgcgta tacttcatct ttgcttggca gcatagcagg tgttggctgg
     2881 actgctggct tatcctcctt tgctgctatt ccatttgcac agagtatctt ttataggtta
     2941 aacggtgttg gcattactca acaggttctt tcagagaacc aaaagcttat tgccaataag
     3001 tttaatcagg ctctgggagc tatgcaaaca ggcttcacta caactaatga agcttttcag
     3061 aaggttcagg atgctgtgaa caacaatgca caggctctat ccaaattagc tagcgagcta
     3121 tctaatactt ttggtgctat ttccgcctct attggagaca tcatacaacg tcttgatgtt
     3181 ctcgaacagg acgcccaaat agacagactt attaatggcc gtttgacaac actaaatgct
     3241 tttgttgcac agcagcttgt tcgttccgaa tcagctgctc tttccgctca attggctaaa
     3301 gataaagtca atgagtgtgt caaggcacaa tccaagcgtt ctggattttg cggtcaaggc
     3361 acacatatag tgtcctttgt tgtaaatgcc cctaatggcc tttacttcat gcatgttggt
     3421 tattacccta gcaaccacat tgaggttgtt tctgcttatg gtctttgcga tgcagctaac
     3481 cctactaatt gtatagcccc tgttaatggc tactttatta aaactaataa cactaggatt
     3541 gttgatgagt ggtcatatac tggctcgtcc ttctatgcac ctgagcccat tacctccctt
     3601 aatactaagt atgttgcacc acaggtgaca taccaaaaca tttctactaa cctccctcct
     3661 cctcttctcg gcaattccac cgggattgac ttccaagatg agttggatga gtttttcaaa
     3721 aatgttagca ccagtatacc taattttggt tccctaacac agattaatac tacattactc
     3781 gatcttacct acgagatgtt gtctcttcaa caagttgtta aagcccttaa tgagtcttac
     3841 atagacctta aagagcttgg caattatact tattacaaca aatggccgtg gtacatttgg
     3901 cttggtttca ttgctgggct tgttgcctta gctctatgcg tcttcttcat actgtgctgc
     3961 actggttgtg gcacaaactg tatgggaaaa cttaagtgta atcgttgttg tgatagatac
     4021 gaggaatacg acctcgagcc gcataaggtt catgttcact aa
//
