LOCUS       sequence-32694-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Collins JJ, Hou X, Romanova EV, Lambrus BG, Miller CM, Saberi A, 
            Sweedler JV, Newmark PA
  TITLE     Genome-wide analyses reveal a role for peptide hormones in planarian
            germline development.
  JOURNAL   PLoS Biol. 2010  . 8(10):e1000509.
  PUBMED    20967238
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2010  . 8(10):e1000509."
COMMENT     Sequence Label: pJC138.3-npp-3
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(287..305)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(288..305)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(308..327)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(309..327)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      341..388
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(400..417)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(651..670)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atctaatgag ttcagccgaa tcgtgtaaga actatggctc tttttgagtc gtcattattg
       61 agaaaatatc gccttgatcc tcgttttccg tatctcgtca gtaagatcgc tcttttaccg
      121 gtttggtagt ttaattcgtg tttactgatc ggatcaagaa gttcatatcg attttcatat
      181 tgatctggca tctgatcggc aaattgaatg taaaagcaca aacagagaac aatcgataaa
      241 tttatgataa aatatgggaa ttgttttgcc atcattcaag gtcacttcta tagtgtcacc
      301 taaatctccc tatagtgagt cgtattagga tccggcgcgc ctagcataac cccttggggc
      361 ctctaaacgg gtcttgaggg gttttttgac gcgtgcttcc tcgctcactg actcgctgcg
      421 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      481 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      541 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      601 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      661 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      721 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgta
//
