LOCUS       sequence-32696-e         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Collins JJ, Hou X, Romanova EV, Lambrus BG, Miller CM, Saberi A, 
            Sweedler JV, Newmark PA
  TITLE     Genome-wide analyses reveal a role for peptide hormones in planarian
            germline development.
  JOURNAL   PLoS Biol. 2010  . 8(10):e1000509.
  PUBMED    20967238
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2010  . 8(10):e1000509."
COMMENT     Sequence Label: pJC136.4-spp-8
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(270..288)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(271..288)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(291..310)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(292..310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      324..371
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(383..400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atacgactca ctataggttt tttttttttt ttttaaaaca tgatgaaaca tttattaatg
       61 agaaaaatgt tcatttttat ctcaggttgg caaaccctga cccaaatgtc atcggatcta
      121 ttagtctctt ctccagcatt cgcttataca gaagcatatt tctgttgaat ccgaatccca
      181 tagtccgctt ctctacctct cctaaggctt caaaagcaca aacagccaga aaaatagcta
      241 gtaaaataat aattttcatg aaggtcactt ctatagtgtc acctaaatct ccctatagtg
      301 agtcgtatta ggatcgggcg cgcctagcat aaccccttgg ggcctctaaa cgggtcttga
      361 ggggtttttt gacgcgtgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      421 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      481 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      541 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      601 caagtcagag gtggcgaaac ccgacaggac tataaagata cca
//
