LOCUS       sequence-326978-        6870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yEVenus under the control of PHO5 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Gligorovski V, Sadeghi A, Rahi SJ
  TITLE     Multidimensional characterization of inducible promoters and a 
            highly light-sensitive LOV-transcription factor.
  JOURNAL   Nat Commun. 2023 Jun 27;14(1):3810. doi: 10.1038/s41467-023-38959-8.
  PUBMED    37369667
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-06-27"; volume: "14(1):3810. doi"; pages: " 
            10.1038/s41467-023-38959-8"
COMMENT     Sequence Label: pVG46
FEATURES             Location/Qualifiers
     source          1..6870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1048..1761
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     terminator      2319..2506
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2374..2398)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2574..2590)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2575..2591)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2615..2634)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2616..2634)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2644..2661)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2644..2660)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2653..2675)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2801..3256
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2888..2907)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3098..3119
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3387..4190)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4191..4406)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(4430..4449)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4549..4571
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4609..4627)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4695..4799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4800..4868,4869..5660)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5018..5037)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5831..6419
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6320..6339
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6573..6590
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6707..6728
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6743..6748,6749..6766,6767..6773)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6781..6797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6786..6808
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6805..6821
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6805..6821
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6840..6860
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6842..6860
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tggagctcca ccgcggtggc ggccgccagc tgaagcttcg tacgcaatgt tccttggtta
       61 tcccatcgcc aataattttt atttttacca ctgttgaaga agcgaaagaa aaaaaaaggg
      121 aaaatcaaaa cattccctgt gcactaatag aagaaaacaa gagactccgt ccctctttag
      181 tgagaaaatt gaccagagat ggtttttgtc catcttttcg caaaaaatta gttctatttt
      241 ttacacatcg gactgataag ttactactgc acattggcat tagctaggag ggcatccaag
      301 taataattgc gagaaacgtg acccaacttt gttgtaggtc cgctccttct aataatcgct
      361 tgtatctcta catatgttct atttactgac cgaaagtagc tcgctacaat aataatgttg
      421 acctgatgtc agtccccacg ctaatagcgg cgtgtcgcac gctctcttta caggacgccg
      481 gagaccggca ttacaaggat ccgaaagttg tattcaacaa gaatgcgcaa atatgtcaac
      541 gtatttggaa gtcatcttat gtgcgctgct ttaatgtttt ctcatgtaag cggacgtcgt
      601 ctataaactt caaacgaagg taaaaggttc atagcgcttt ttctttgtct gcacaaagaa
      661 atatatatta aattagcacg ttttcgcata gaacgcaact gcacaatgcc aaaaaaagta
      721 aaagtgatta aaagagttaa ttgaataggc aatctctaaa tgaatcgata caaccttggc
      781 actcacacgt gggactagca cagactaaat ttatgattct ggtccctgtt ttcgaagaga
      841 tcgcacatgc caaattatca aattggtcac cttacttggc aaggcatata cccatttggg
      901 ataagggtaa acatctttga attgtcgaaa tgaaacgtat ataagcgctg atgttttgct
      961 aagtcgaggt tagtatggct tcatctctca tgagaataag aacaacaaca aatagagcaa
     1021 gcaaattcga gattaccatt aattaacatg tctaaaggtg aagaattatt cactggtgtt
     1081 gtcccaattt tggttgaatt agatggtgat gttaatggtc acaaattttc tgtctccggt
     1141 gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa aattgatttg tactactggt
     1201 aaattgccag ttccatggcc aaccttagtc actactttag gttatggttt gcaatgtttt
     1261 gctagatacc cagatcatat gaaacaacat gactttttca agtctgccat gccagaaggt
     1321 tatgttcaag aaagaactat ttttttcaaa gatgacggta actacaagac cagagctgaa
     1381 gtcaagtttg aaggtgatac cttagttaat agaatcgaat taaaaggtat tgattttaaa
     1441 gaagatggta acattttagg tcacaaattg gaatacaact ataactctca caatgtttac
     1501 atcactgctg acaaacaaaa gaatggtatc aaagctaact tcaaaattag acacaacatt
     1561 gaagatggtg gtgttcaatt agctgaccat tatcaacaaa atactccaat tggtgatggt
     1621 ccagtcttgt taccagacaa ccattactta tcctatcaat ctgccttatc caaagatcca
     1681 aacgaaaaga gagaccacat ggtcttgtta gaatttgtta ctgctgctgg tattacccat
     1741 ggtattgatg aattgtacaa agcatccaac ttgaacattt cgagaaagct taccatatca
     1801 accccatcat gctctttcga aaattcaaat agcacatcca ttccttcgcc cgcttcctca
     1861 tctcaaagcc acactccaat gagaaacatg agctcactct ctgataacag cgttttcagc
     1921 cggaatatgg aacaatcatc accaatcact ccaagtatgt accaatttgg tcagcagcag
     1981 tcaaacagta tatgtggtag caccgttagt gtgaatagtc tggtgaatac aaataacaaa
     2041 caaaggatct acgaacaaat cacgggtcct aacagcaata acgcaaccaa tgattatatt
     2101 gatttgctaa acctaaatga gtctaacaag gaaaaccaaa atcccgcaac ggcgcattac
     2161 ctcaatgggg gcccacccaa gacaagcttc attaaccatg gaatgttccc ctcgccaact
     2221 gggaccataa atagcggtaa atctagcagt gcctcatctt taatttcttt tggtatgggc
     2281 aatacccaag taatataggg cgcgccactt ctaaataagc gaatttctta tgatttatga
     2341 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
     2401 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
     2461 ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga tccgctaggg
     2521 ataacagggt aatatagatc ccccgggctg caggaattcg atatcaagct tatcgatacc
     2581 gtcgacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg
     2641 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     2701 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     2761 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
     2821 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2881 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2941 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3001 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3061 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3121 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3181 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3241 ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3301 accgcatagg gtaataactg atataattaa attgaagctc taatttgtga gtttagtata
     3361 catgcattta cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct
     3421 tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat
     3481 agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta
     3541 tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac
     3601 caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa
     3661 tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag
     3721 cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct
     3781 gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg
     3841 tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca
     3901 atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt
     3961 agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt
     4021 aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca
     4081 tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca
     4141 acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt
     4201 cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt
     4261 tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct
     4321 tccttctgtt cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa
     4381 gaataaaaaa aaaatgatga attgaaaagg tggtatggtg cactctcagt acaatctgct
     4441 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     4501 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     4561 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     4621 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     4681 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4741 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4801 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4861 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4921 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4981 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5041 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5101 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5161 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5221 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5281 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5341 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5401 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5461 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5521 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5581 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5641 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5701 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5761 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5821 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5881 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5941 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6001 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6061 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6121 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6181 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6241 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6301 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6361 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6421 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6481 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6541 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6601 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6661 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     6721 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6781 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     6841 caattaaccc tcactaaagg gaacaaaagc
//
