LOCUS       sequence-326983-        6392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yEVenus under the control of ARG3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Gligorovski V, Sadeghi A, Rahi SJ
  TITLE     Multidimensional characterization of inducible promoters and a 
            highly light-sensitive LOV-transcription factor.
  JOURNAL   Nat Commun. 2023 Jun 27;14(1):3810. doi: 10.1038/s41467-023-38959-8.
  PUBMED    37369667
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-06-27"; volume: "14(1):3810. doi"; pages: " 
            10.1038/s41467-023-38959-8"
COMMENT     Sequence Label: pVG88
FEATURES             Location/Qualifiers
     source          1..6392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      987..1174
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1042..1066)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1242..1258)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1243..1259)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1283..1302)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1284..1302)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1312..1329)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1312..1328)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1321..1343)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1469..1924
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1556..1575)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1766..1787
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2055..2858)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(2859..3074)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(3098..3117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3217..3239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3277..3295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3363..3467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3468..3536,3537..4328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3686..3705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4499..5087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4988..5007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5241..5258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5375..5396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5411..5416,5417..5434,5435..5441)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5449..5465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5454..5476
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5473..5489
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5473..5489
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5508..5528
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5510..5528
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             join(6108..6392,1..429)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 agaactattt ttttcaaaga tgacggtaac tacaagacca gagctgaagt caagtttgaa
       61 ggtgatacct tagttaatag aatcgaatta aaaggtattg attttaaaga agatggtaac
      121 attttaggtc acaaattgga atacaactat aactctcaca atgtttacat cactgctgac
      181 aaacaaaaga atggtatcaa agctaacttc aaaattagac acaacattga agatggtggt
      241 gttcaattag ctgaccatta tcaacaaaat actccaattg gtgatggtcc agtcttgtta
      301 ccagacaacc attacttatc ctatcaatct gccttatcca aagatccaaa cgaaaagaga
      361 gaccacatgg tcttgttaga atttgttact gctgctggta ttacccatgg tattgatgaa
      421 ttgtacaaag catccaactt gaacatttcg agaaagctta ccatatcaac cccatcatgc
      481 tctttcgaaa attcaaatag cacatccatt ccttcgcccg cttcctcatc tcaaagccac
      541 actccaatga gaaacatgag ctcactctct gataacagcg ttttcagccg gaatatggaa
      601 caatcatcac caatcactcc aagtatgtac caatttggtc agcagcagtc aaacagtata
      661 tgtggtagca ccgttagtgt gaatagtctg gtgaatacaa ataacaaaca aaggatctac
      721 gaacaaatca cgggtcctaa cagcaataac gcaaccaatg attatattga tttgctaaac
      781 ctaaatgagt ctaacaagga aaaccaaaat cccgcaacgg cgcattacct caatgggggc
      841 ccacccaaga caagcttcat taaccatgga atgttcccct cgccaactgg gaccataaat
      901 agcggtaaat ctagcagtgc ctcatcttta atttcttttg gtatgggcaa tacccaagta
      961 atatagggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta
     1021 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     1081 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt
     1141 atgaggtcgc tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtaa
     1201 tatagatccc ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag
     1261 ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     1321 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     1381 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     1441 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     1501 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     1561 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     1621 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     1681 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     1741 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     1801 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     1861 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     1921 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagggt
     1981 aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact
     2041 tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct
     2101 tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca
     2161 acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc
     2221 aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct
     2281 tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc
     2341 ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac
     2401 aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc
     2461 aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct
     2521 gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat
     2581 tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact
     2641 gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg
     2701 ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca
     2761 cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga
     2821 tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg tttcctgcag
     2881 gtttttgttc tgtgcagttg ggttaagaat actgggcaat ttcatgtttc ttcaacacta
     2941 catatgcgta tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg
     3001 gagattaccg aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa
     3061 aatgatgaat tgaaaaggtg gtatggtgca ctctcagtac aatctgctct gatgccgcat
     3121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     3181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     3301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4621 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     5281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     5341 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     5401 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     5461 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     5521 actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgccagctg aagcttcgta
     5581 cgctgcaggt cgggatcctc ttctaagaaa aaatatttag atcatattat tttagataac
     5641 cgagacatcg ttagcaacca tgactccagt aaacaaaaat tcaagatcca gaatattttg
     5701 aactcgacct tctaacatta cgctccttcg tattactcat tcagctcttc ctctgatagc
     5761 agtgaatttt cgagggtcac gtcgtgactc atatgctttc ttgttccgtt tcgtttcgag
     5821 atgacaaaaa actggtcatt ttttccgtta agtgcaactc acagcagtat cggccgctga
     5881 gaaatgcccg gacaaatttt tttgagccgg attggtcacc gtttctttct tcggcgcggc
     5941 ttcccattcc cgtccatcca aaaaaatcta cctatataaa tcgacttttc acctctaaag
     6001 gcagtttatt ccttgtatgt cctttaagta cagttaataa cgagcaattt tttttttttt
     6061 ttttagccat ctacccatca acttgtacac tcgttacctt aattaacatg tctaaaggtg
     6121 aagaattatt cactggtgtt gtcccaattt tggttgaatt agatggtgat gttaatggtc
     6181 acaaattttc tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa
     6241 aattgatttg tactactggt aaattgccag ttccatggcc aaccttagtc actactttag
     6301 gttatggttt gcaatgtttt gctagatacc cagatcatat gaaacaacat gactttttca
     6361 agtctgccat gccagaaggt tatgttcaag aa
//
