LOCUS       sequence-326986-        6332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yEVenus under the control of GALL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Gligorovski V, Sadeghi A, Rahi SJ
  TITLE     Multidimensional characterization of inducible promoters and a 
            highly light-sensitive LOV-transcription factor.
  JOURNAL   Nat Commun. 2023 Jun 27;14(1):3810. doi: 10.1038/s41467-023-38959-8.
  PUBMED    37369667
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-06-27"; volume: "14(1):3810. doi"; pages: " 
            10.1038/s41467-023-38959-8"
COMMENT     Sequence Label: pVG10
FEATURES             Location/Qualifiers
     source          1..6332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(15..20,21..38,39..45)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    53..69
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     58..80
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     77..93
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     77..93
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     112..132
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        114..132
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     595..618
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             652..1365
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     terminator      1923..2110
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1978..2002)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2178..2194)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2179..2195)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2219..2238)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2220..2238)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2248..2265)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2248..2264)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2257..2279)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2405..2860
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2492..2511)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2702..2723
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2991..3794)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3795..4010)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(4034..4053)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4153..4175
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4213..4231)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4299..4403
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4404..4472,4473..5264)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4622..4641)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5435..6023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5924..5943
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6177..6194
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6311..6332
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
       61 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      121 cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgcca gctgaagctt
      181 cgtacgctgc aggtcgacgg atccgggaca gccctccgaa ggaagactct cctccgtgcg
      241 tcctcgtctt caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg
      301 aacaataaag attctacaat actagctttt atggttatga agaggaaaaa ttggcagtaa
      361 cctggcccca caaaccttca aatgaacgaa tcaaattaac aaccatagga tgataatgcg
      421 attagttttt tagccttatt tctggggtaa ttaatcagcg aagcgatgat ttttgatcta
      481 ttaacggata tataaatgca aaaactgcat aaccacttta actaatactt tcaacatttt
      541 cggtttgtat tacttcttat tcaaatgtaa taaaagtatc aacaaaaaat tgttaatata
      601 cctctatact ttaacgtcaa ggagaaaaaa ccccggattc tattaattaa catgtctaaa
      661 ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat
      721 ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg taaattgacc
      781 ttaaaattga tttgtactac tggtaaattg ccagttccat ggccaacctt agtcactact
      841 ttaggttatg gtttgcaatg ttttgctaga tacccagatc atatgaaaca acatgacttt
      901 ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac
      961 ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc
     1021 gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa attggaatac
     1081 aactataact ctcacaatgt ttacatcact gctgacaaac aaaagaatgg tatcaaagct
     1141 aacttcaaaa ttagacacaa cattgaagat ggtggtgttc aattagctga ccattatcaa
     1201 caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta cttatcctat
     1261 caatctgcct tatccaaaga tccaaacgaa aagagagacc acatggtctt gttagaattt
     1321 gttactgctg ctggtattac ccatggtatt gatgaattgt acaaagcatc caacttgaac
     1381 atttcgagaa agcttaccat atcaacccca tcatgctctt tcgaaaattc aaatagcaca
     1441 tccattcctt cgcccgcttc ctcatctcaa agccacactc caatgagaaa catgagctca
     1501 ctctctgata acagcgtttt cagccggaat atggaacaat catcaccaat cactccaagt
     1561 atgtaccaat ttggtcagca gcagtcaaac agtatatgtg gtagcaccgt tagtgtgaat
     1621 agtctggtga atacaaataa caaacaaagg atctacgaac aaatcacggg tcctaacagc
     1681 aataacgcaa ccaatgatta tattgatttg ctaaacctaa atgagtctaa caaggaaaac
     1741 caaaatcccg caacggcgca ttacctcaat gggggcccac ccaagacaag cttcattaac
     1801 catggaatgt tcccctcgcc aactgggacc ataaatagcg gtaaatctag cagtgcctca
     1861 tctttaattt cttttggtat gggcaatacc caagtaatat agggcgcgcc acttctaaat
     1921 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     1981 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     2041 ctttcctgta ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca
     2101 cctctaccgg cagatccgct agggataaca gggtaatata gatcccccgg gctgcaggaa
     2161 ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc
     2221 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     2281 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     2341 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2401 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2461 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2521 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2581 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     2641 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2701 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     2761 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2821 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
     2881 ttacgcatct gtgcggtatt tcacaccgca tagggtaata actgatataa ttaaattgaa
     2941 gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc
     3001 tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc
     3061 ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta
     3121 gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa
     3181 cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt
     3241 tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta
     3301 gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct
     3361 ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc
     3421 accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag
     3481 tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa
     3541 ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc
     3601 aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc
     3661 agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc
     3721 atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat
     3781 gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg cagttgggtt
     3841 aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata taccaatcta
     3901 agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt
     3961 caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa aaggtggtat
     4021 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     4081 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4141 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4201 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4261 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4321 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4381 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4441 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4501 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4561 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4621 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4681 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4741 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4801 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4861 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4921 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4981 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5041 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5101 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5161 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5221 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5281 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5341 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5401 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5461 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5521 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5581 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5641 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5701 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5761 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5821 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5881 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5941 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6001 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6061 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6121 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6181 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6241 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6301 gcaacgcaat taatgtgagt tagctcactc at
//
