LOCUS       sequence-326991-       12351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This is the FIV PPR based second generation packaging vector, part 
            of the FELIX vector system from Nolan lab.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12351)
  TITLE     Nolan Lab Plasmids
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCPRDEnv
FEATURES             Location/Qualifiers
     source          1..12351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      173..761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     662..681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        972..1351
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1352..1555
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1506..1526
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    10299..10420
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10310..10329)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10364..10383
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      10601..11056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10688..10707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10898..10919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11123..11142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11242..11264
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11302..11320)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11388..11492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11493..11561,11562..12351,1..2)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11711..11730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
       61 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      121 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      181 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      241 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      301 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      361 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      421 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      481 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      541 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      601 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      661 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      721 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      781 ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag atcttcaata
      841 ttggccatta gccatattat tcattggtta tatagcataa atcaatattg gctattggcc
      901 attgcatacg ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg
      961 accgccatgt tggcattgat tattgactag ttattaatag taatcaatta cggggtcatt
     1021 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     1081 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     1141 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     1201 ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa
     1261 atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta
     1321 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg
     1381 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     1441 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc
     1501 gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcataa
     1561 tcaagtcact gtttaaaggc ccagataaat tacatttggt gactcttcgc ggaccttcaa
     1621 gccaggagat tcgccgaggg acagctaaca aggtaggaga gactctacag caacatgggg
     1681 aatggacagg ggcgagattg gaaaatggcc attaagagat gtagtaatgt tgctgtagga
     1741 gtagggggga agagtaaaaa atttggagag gggaatttta ggtgggccat aagaatggct
     1801 aatgtatcta caggacgaga acctggtgat ataccagaga ctttagatca actaaggttg
     1861 gttatttgcg atttacaaga aagaagagaa aaatttggat ctagcaaaga aattgacatg
     1921 gcaattacaa cattaaaagt ctttgcagta gtgggacttt taaatatgac agtgtctact
     1981 gctgctgcag ctgaaaatat gtatactcag atgggattag acactagacc gtctacaaaa
     2041 gaagcgggag gaaaagagga aggccctcca caggcatatc ctattcaaac agtaaatgga
     2101 gcaccacaat atgtagcact tgacccaaaa atggtgtcca tttttatgga aaaggcaaga
     2161 gagggattag gaggtgagga agttcaacta tggtttacag ccttctctgc aaatttaaca
     2221 cctactgaca tggccacatt aataatggcc gcacccgggt gcgctgcaga taaagaaata
     2281 ttggatgaaa gcttaaagca attgacagca gaatatgatc ggacacatcc ccctgatggt
     2341 cctagaccat taccctattt tactgcagca gaaattatgg gtataggatt aactcaagaa
     2401 caacaagcag aagcaagatt tgcaccagct aggatgcaat gtagagcatg gtatcttgag
     2461 gcattaggaa aattagccgc cataaaggct aaatctcctc gagctgtgca gttaagacaa
     2521 ggagctaagg aagattattc atcctttata gacagattgt ttgcccaaat agatcaagaa
     2581 caaaatacag ctgaagttaa gttatatcta aaacagtcat taagcatagc taatgctaat
     2641 gcagaatgca aaaaggcaat gagtcatctt aagccagaaa gtaccctaga agaaaagttg
     2701 agagcttgtc aagagatagg atccccagga tataaaatgc aactcttggc agaagctctt
     2761 acaaaagttc aagtagtgca atcaaaagga tcaggaccag tgtgttttaa ttgtaaaaaa
     2821 ccagggcatc tagcaagaca gtgtagagat gtgaaaaaat gtaataaatg tggaaaacct
     2881 ggtcatttag ctgccaaatg ttggcaaggt ggtaaaagaa attcgggaaa ctggaaggcg
     2941 gggcgagctg cagccccagt gaatcaagtg cagcaaacag taatgccatc tgcacctcca
     3001 atggaggaaa aattattgga tttataaatt acaataaagt aggtactact acatcattag
     3061 aaaagaggcc agaaatactt atatttgtga atgggtaccc tataaaattt ttattagata
     3121 caggagcaga tataacaatt ttaaatagga gagattttca agtaaaaaac tctatagaaa
     3181 atggaagaca aaatatgatt ggagtagggg gaggaaagag aggaacaaat tatatcaatg
     3241 tgcatttaga gattagagat gaaaattaca agacacaatg tatatttggc aatgtttgtg
     3301 tcttagaaga taactcatta atacaaccat tattagggag agataatatg attaaattta
     3361 atatcaggtt agtaatggct caaatttctg ataagattcc aatagtaaaa gtaaagatga
     3421 aggatcctaa taaaggacct caaataaaac aatggccatt atcaaatgaa aaaattgaag
     3481 ctttaacaga aatagtagaa agactagaaa gggaagggaa agtaaaaaga gcagatccaa
     3541 ataatccatg gaatacacca gtatttgcta taaaaaagaa aagtggaaaa tggaggatgc
     3601 tcatagattt tagagaattg aacaaattaa ctgagaaagg agcagaagtc cagttgggac
     3661 tacctcaccc tgctggttta caaatgaaaa aacaaataac agtattagat ataggggatg
     3721 catatttcac cattcccctt gacccagatt atgctcctta tacagcattt actttaccta
     3781 ggaagaataa tgcgggacca ggaagaagat ttgtgtggtg tagtctacca caaggctgga
     3841 ttttaagtcc attgatatat caaagtacat tagataatat aatacaacct tttattagac
     3901 aaaatcctca attagatatt tatcaatata tggatgacat ttatatagga tcaaacttaa
     3961 gtaaaaagga gcataaagaa aaagtagaag aattaagaaa attactatta tggtggggat
     4021 ttgaaactcc agaggataaa ttacaggaag aacccccata taaatggatg ggttatgaat
     4081 tacatccatt aacatggaca atacaacaga aacagttaga aattccagaa aagcctacat
     4141 taaatgaatt acaaaaatta gcaggaaaaa ttaattgggc tagccaaact attccagaat
     4201 taagtataaa agcattaact aacatgatga gaggaaatca aaacctaaat tcaacaagag
     4261 agtggactga ggaagctaga ctagaagtac agaaggccaa aagggctatt gaagaacaag
     4321 tacaactagg atattatgac cctagtaaag aattgtatgc taaattaagc ttagtgggac
     4381 cacatcaaat aagttatcaa gtatatcaga agtgtccaga aaagatctta tggtatggaa
     4441 aaatgagtag gcaaaagaaa aaggcagaaa atacgtgtga tatagcgtta agagcatgct
     4501 acaaaataag ggaagaatcc attataagaa taggaaaaga accaagatat gaaataccta
     4561 cttctagaga agcctgggaa tcaaatttaa ttaattcacc atatcttaaa gccccacctc
     4621 cagaagtaga ctatatccat gctgctttaa acataaaaag agcactaagt atgataaaag
     4681 atgctccaat atcaggagca gaaacgtggt atatagatgg aggtagaaag ctaggaaaag
     4741 cagcaaaagc agcctattgg acagatacag gaaagtggca agtaatggaa ttagaaggta
     4801 gtaatcagaa ggcggaaata caagcattat tattggcatt aaaggcagga ccagaggaaa
     4861 tgaatattat aacagattct cagtatatga taaatattct tagtcaacaa ccagataaga
     4921 tggaaggaat ctggcaagaa gttttagaag aattggaaaa gaaaacagca atatttatag
     4981 attgggtccc aggacataaa ggtattccag gaaatgagga agtagataag ctttgtcaaa
     5041 caatgatgat aatagaaggg gatgggatat tagataaaag aacagaagat gcaggatatg
     5101 atttattagc tgcaaaagaa atacatctat taccaggaga ggtaaaagta ataccaacgg
     5161 gagtaaaact aatgttgcct aaaggacatt ggggattaat aatgggaaaa agctcgatag
     5221 ggagtaaagg attggatgta ttaggagggg taatagatga aggatatcga ggtgaaattg
     5281 gagtaataat gattaattta tcaaaaaaat caatcacttt gttggaacaa cagaagatag
     5341 cacaattaat aatattgcct cataaacatg aagcattaga acaggggaaa gtagtaatgg
     5401 attcagagag aggagaaaaa ggttatgggt caacaggagt attctcctct tgggttgaca
     5461 gaattgagga agcagaaaca aatcatgaaa aatttcactc agatccgcaa tacttaagga
     5521 ctgaatttaa tttacccaag atggtggcag aagagataag acgaaaatgc cctgtatgta
     5581 ggattagagg agaacaagtg ggagggcaat tgaagatagg gcctggtatc tggcaaatgg
     5641 attgcacaca ctttgatggc aaaataattc ttgtggctat acatgtggaa tcaggatata
     5701 tatgggcaca aataatctct caagaaactg ctgattgtac agttaaagct gtcttacaat
     5761 tattgagtgc tcataatgtt acggagttac aaacagataa tggaccaaat tttaaaaatc
     5821 aaaagatgga aggagtactc aattatatgg gtgtgaaaca taagtttggt atcccaggaa
     5881 acccacaatc acaagcatta gttgaaaatg taaatcagac attaaaagtc tgggttcaca
     5941 aatttttgcc tgaaacaacc tccttggata atgcattagc tctcgctgtg cattgtctca
     6001 attttaaaca aaggggtaga ataggaggga tggcccctta tgaattatta gcacaacaag
     6061 aatccttaag aatacaagat tatttctctg caataccaca aaaattgcaa gcacaatgga
     6121 tttattataa agatcaaaaa gataagaaat ggaaagggcc aatgagagta gaatactggg
     6181 gacaaggatc agtgttatta aaggatgaag agaagggata ttttcttata cctaggagac
     6241 acgtaaagag agtcccagaa ccctgcgctc ttcctgaagg ggatgagtga cgaagattgg
     6301 caggtaagta gaagactctt tgcagtgctc caaggagggg tatataacgc tatgctatat
     6361 atatctagac tacctcagga cgaaagagaa aaatataaaa aggatttcaa gaaaagactt
     6421 ttagatacag aaacaggatt tataaaaaga ctaaggaaag ctgaaggaat aaaatggagc
     6481 tttcatacta gagattatca tgtaggatat gttagagaaa tggtagcagg acccactaca
     6541 ccacatagtc taaggctgta tgtgtatata agtaatccac tatggcattc tcagtatcgt
     6601 ccaggcttgg taaattttaa taaggaatgg ccttttgtaa atctatggat aaaaacagga
     6661 tttatgtggg atgatattga aaaacaaaat atttgtatag gaggagaagt ttcaccagga
     6721 tggggacctg ggatgatagg tatagcgata aaagctttta gttgtggcga aagaaagatt
     6781 gaggctactc ctgtaatgat tataagagga gaaataaatc caaaaaaatg gtgtggagac
     6841 tgttggaatt tgatgtgtct tagaaactca cctccagaga ctttacaaag gctcgctatg
     6901 ttggcatgtg gagtacaggc taagagctgg cgaggatgct gtaatcaacg ttttgtttct
     6961 ccttacagaa cacctgctga tttagaggtt attcaatcca aacccggctg gtgcatgtta
     7021 tggcgaggaa aactgtgaat ggaagtaata cggatattta ataaggtcgc tgaaagatta
     7081 gacaaggaag cagccatcag gatatttgta ttagcacatc aattagagag ggataaattg
     7141 attagacttc tgcaaggact actttggaga ctgagattta gaaaacctaa atcaaaagat
     7201 tgtttatgtt ggttttgctg cagattatat tattggcagt tgcagtctac attatccata
     7261 gatactgctt agaaatattt ataataatat ttcatttgca acaataatta tggcagaagg
     7321 gtttgcagcc aatagacaat ggatagggcc agaagaagct gaagagctat tagattttga
     7381 taaagcaaca caaatgaatg aagaagggcc actaaatcca ggagtaaacc catttagagt
     7441 acctgcagta acagaagcag acaagcaaga atattgtaag atattacaac cccgattaca
     7501 agagataagg aatgaaattc aagaagtaaa actagaagaa ggaaatgcag gtaagtttag
     7561 aagagcaaga ttcttgagat attctgatga aagtatatta tccttaattc atttgttcat
     7621 agggtattgt acatacttag taaatagaag gaggttagga tctttaaggc atgacataaa
     7681 tatagaagcg cctcaagaag agcagtatag cagtagagag cagggcacaa ctgagaatat
     7741 aaaatatggt agacgatgct tgataggaac agcaagtctg tacttgttgc tttttatagg
     7801 agtggcaata tatttaggta caaccaatgc tcagatagta tggagacttc caccattagt
     7861 agtcccagta gaagaatcag aaataatttt ttgggattgt tgggcaccag aggagcccgc
     7921 ctgtcaagac tttcttgggg caatgataca tctaaaagct agtacaaata taagtataca
     7981 agaaggacct accttgggga attgggctag agaaatatgg ggaacattat tcaaaaaagc
     8041 tactagacaa tgtaggagaa ataaaatatg gaaaaggtgg aatgaaacta taacaggacc
     8101 agtaggatgt gctaataata catgttataa tatctctgta ataatacctg attatcaatg
     8161 ttatctagat agagtagata cttggttaca agggaaagta aatatatcat tatgcctaac
     8221 aggaggaaaa atgttgtata atagagatac aaaacaatta agctattgta cagacccatt
     8281 acaaatccca ctgatcgatc aattatacat ttgggcctaa tcaaacatgt atgtggaaca
     8341 cttcacagat tcaagacccg gagataccaa aatgtggatg gtggaatcaa atagcctatt
     8401 ataacagttg tagatgggaa agcacaaatg taaagtttta ttgtcaaaga acacagagtc
     8461 agcctggaac atggattaga acaatctcat catggagaca aaagaataga tgggaatgga
     8521 gaccagactt tgaaagcgaa aaagttaaaa tatcattaca atgtaatagt acacataatt
     8581 taacttttgc aatgagaagt tcaggagatt atggagaagt aatgggagct tggatagaat
     8641 ttggatgtca taggaacaaa tcaagattcc atactgaagc aaggtttaga attagatgta
     8701 gatggaatgt aggggataat acctcactca ttgatacatg tggaaaaaat ctaaatgttt
     8761 caggtgccaa tcctgtagat tgtaccatgt atgcaaataa aatgtataac tgttccttac
     8821 aaaacgggtt tactatgaag gtagatgacc ttattatgca tttcaatatg acaaaagcag
     8881 tagaaatgta taacattgct gggaattggt cttgtaaatc tgatttacca caaaattggg
     8941 gatatatgaa ttgtaattgt acgaatggta cgagtaatga caataaaatg gcatgtcctg
     9001 aagataaggg tatcttaaga aattggtata atccagtagc aggattaaga caagcattag
     9061 aaaaatatca agtggtaaaa cagccagaat atatagtagt accaacagaa gttatgacct
     9121 ataaatacaa acagaaaaga gcagcaattc atattatgtt agctcttgca acagtattgt
     9181 ctatagctgg ggcaggaaca ggtgctacag caattgggat ggtgactcaa tatcagcaag
     9241 ttttagctac tcatcaagaa gcattggata aaataactga agcattgaaa ataaataatt
     9301 taaggttagt tactttagag catcaaatgt tagtcatagg attgaaagta gaagctatag
     9361 aaaaattttt atatactgct tttgctatgc aagaactagg atgtaatcaa aatcaattct
     9421 tttgtgaaat tcccaaagag ctatggctaa ggtataatat gacattaaat caaacaattt
     9481 ggaatcatgg aaatataact ttaggggaat ggtacaatca gacaaaatat ttacaacaaa
     9541 aattttatga aataattatg gatatagaac aaaataatgt acaaggaaaa caaggattac
     9601 aaaaattaca aaattggcaa gattggatgg gatggatagg aaaaatacct caatacttaa
     9661 agggactctt gggaggcatt ttgggaatag gtttgggaat tctattattg attttatgtt
     9721 tacccacttt agttgattgt attagaaatt gtattagtaa agttctagga tatacagtaa
     9781 tcgcaatgcc tgagatagat gatgaagaag aaacggtaca aatggaattg aggaaaaatg
     9841 gcaggcaatg tggcatgtct gaaaaagagg aggaatgatg gagtgcctca gaactgctta
     9901 atgcaggaga ggtgctgagc tgatttcttc cctttgagga agatatgtca tatgaatcca
     9961 ttttgaatca aaataataag tatttgtatt ataaggtaaa atgaaaaaga aaagacaaag
    10021 aagaagaaga aagaagaagg cctttaagaa aatgatgaca gatttagaag accgcttcag
    10081 aaaactattc ggctcaccct ctaaagatga atacacagaa attgagatag aagaagaccc
    10141 tcctaaaaaa gaaaaaaggg tggactggga tgagtattgg gaccctgaag aaatagaaag
    10201 aatgcttatg gactaagaac tgtcacaaac aaatgataaa tggaaacagc tgaacatgac
    10261 tcatagttaa agcgggcggc cgcttcgagc agacatgata agatacattg atgagtttgg
    10321 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
    10381 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
    10441 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta
    10501 caaatgtggt aaaatcgata aggatccggg ctggcgtaat agcgaagagg cccgcaccga
    10561 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
    10621 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    10681 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    10741 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    10801 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    10861 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    10921 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    10981 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    11041 ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
    11101 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
    11161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
    11221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
    11281 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
    11341 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    11401 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    11461 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    11521 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    11581 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    11641 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    11701 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    11761 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    11821 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    11881 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    11941 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    12001 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    12061 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    12121 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    12181 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    12241 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    12301 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg t
//
