LOCUS       sequence-326996-       12617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carboxysome operon with point mutation to CbbL, prk; Substitution of
            CsoS2-binding tyrosine with arginine prevents rubisco encapsulation 
            creating rubisco-less carboxysomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12617)
  AUTHORS   Flamholz AI, Dugan E, Blikstad C, Gleizer S, Ben-Nissan R, Amram S, 
            Antonovsky N, Ravishankar S, Noor E, Bar-Even A, Milo R, Savage D
  TITLE     Functional reconstitution of a bacterial CO2 concentrating mechanism
            in E. coli.
  JOURNAL   Elife. 2020 Oct 21;9. pii: 59882. doi: 10.7554/eLife.59882.
  PUBMED    33084575
REFERENCE   2  (bases 1 to 12617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-21"; volume: "9. pii: 59882. doi"; pages: " 
            10.7554/eLife.59882"
COMMENT     Sequence Label: pCB' CbbL Y72R
FEATURES             Location/Qualifiers
     source          1..12617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     168..187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        443..547
                     /gene="bla"
                     /label=AmpR promoter
     CDS             548..1171
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVAQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(621..640)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      complement(1344..1430)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2480..2497)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2543..2598
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    2544..2562
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2574..2592
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     RBS             10977..10988
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(10998..11023,11024..11029,11030..11046,
                     11047..11052,11053..11071))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    11053..11071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(11092..11110)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             11289..12083
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11343..11362)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11953..11972
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      12114..12208
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(12296..12617,1..267)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
       61 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gccctaggtc
      421 tagcgcgaat tgtcgaggga aacgcggaac ccctatttgt ttatttttct aaatacattc
      481 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      541 gaagagtatg tctagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa
      601 tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagcgca
      661 gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga
      721 gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt
      781 tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
      841 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
      901 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg
      961 gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag
     1021 ggaaacacct actactgata gtatgccgcc attattacga caagctatcg aattatttga
     1081 tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga
     1141 aaaacaactt aaatgtgaaa gtgggtctta acagttcctg gatatccgga tgaaggcacg
     1201 aacccagtgg acataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1261 tattcaacat ttccgtgtcg cccttattcc cttttgcttg gattctgcgt ttgtttccgt
     1321 ctacgaactg ctagcggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     1381 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctctag
     1441 cacgcgtacc atgggatccc ccgggctgca ggaattccat ctagacacta gcggcgacgg
     1501 gtgctgcttg ggaggtcaaa cgctcatagg ccgcccgcat tttcaggccg gtcagcactt
     1561 ggaacagacc cgtgccgttg ttcgaacccg ggtaatcttt gtgctgtagc agcaggtggg
     1621 tcaactcacc gtagtactgc gtgtcggtct tgctgaggtg gccctcgacg tagatcatct
     1681 cttcgaggtt ctcgaactga ccgtcgacct caagcactga gacttcgtga ccgtagtagg
     1741 tgtcagggcc gtaggctaag cgaatgcccg gataggagca ggtcagctta cgaccgcagg
     1801 gcgtccactg gatggtcgaa ccttcgtcga acaggtaagc cggctcaaaa ccatcgcggc
     1861 cttcccgttg gatcaactgc acccgcagca ccttgcgctc ggtgtcattg gggattagct
     1921 gggtcggcat gacgcggatg acgatgtccg catggccacg ctggggctca atgtaggcct
     1981 tgaagtcagg gcggcgcgct tcgatcgagg cgaggacatc ttcgtaggag tggccgcgtt
     2041 ctgccatatc gcgttggatc ttccaagcga ttttgacttc gtcatcgatg tcgaggtaaa
     2101 cgctgaaatc gagcagttca cgcacgcgct cgtcgtaaag cggatgcaga ccctcgatca
     2161 caatgatgcg attgggttcg attttttcgg gcggatcgat caagccggtt tcatggttgt
     2221 agatcggctt catgatcgtt tcgccgttct tcaacgcctt gacctgttca tacatcaagt
     2281 caaagttgtt ggcgcgggga tccaaagccg ttacgcctgc ttccttccgg cccttgcgat
     2341 cgagactgtg atagtcatcc aagcagatga ccgtcatcaa ttccgtacca aacaagtcgg
     2401 caaggcgatt taggaaggtt gatttgccgc aaccggagtc accggcaacg ccgatcaaaa
     2461 caacacgatc tggcttgctg tggtgatggt gatgatgcat gcggaattcc ccctcttact
     2521 actggctggc tttttttcac ttttctctat cactgatagg gagtggtaaa ataactctat
     2581 caatgataga gtgtcaacaa aaattaggaa ttaatcatga tttagaaccc ttcagcgcga
     2641 cgcgcacgcg cgttgatgct tgcgattgtg gcctcagccg cccgcatggc ttcttcggtt
     2701 tccgcttcgc tgcccatcat ggtcaggcga ccaaagttac caaaagcacg tacatcgatc
     2761 agagtcacat gagctgcttt ctcggcctcg ttggcagcct gaacaatata acctgccggt
     2821 tctgtttcaa ggatgaacat gcttttgccc ggcaacagca tagagccttt acgcaactgg
     2881 cgattgatca aggtggcatg atcgggggta atcgctcgaa tgatctcttt ccatgcgatg
     2941 cgacaaggca gacgatcttc ttgcgcgtga ttgatttctc ttaaaacggc ctcaccggat
     3001 gcgagaacgt cgctttggtt tcggtaatga atttccatcg agccaaaagc acgctcgact
     3061 acctgttcgc cgagacgaac gttcgtggcc tttagcgcaa tatcactgag gcgatggacg
     3121 gccatgcccg gcgcgacttc aatccacaag caggcatcac ccggaacggg aagaaagcct
     3181 tgcgatgatg tcgcaagata ggatgcaagc tgcggttgaa gcgaatcgat aaacgaatag
     3241 acgcgcaaat caatgttgtt catgcgcatc ttccctacta gacattagct attcagattt
     3301 gcgatacacc acgcggaatc tgagtcttcg ggcgtctcgg gtaggatgat ctcaacttca
     3361 gaatgaacgc gcgcaatgat gtgcgcagca accaggccat cgccaacacg ttcacaggcg
     3421 tcagcacccg cacgaacggc agcatttact gcacctgtct caccacgaac cattacggtc
     3481 acgtaaccgc cgccaacaaa tgatcgtccg accagacgga cttccgccgc tttggtcatg
     3541 gcatctgcgg cttcaatggc aggaaccaat cctcgtgttt caatcatgcc cagggcaata
     3601 ccgtgagtcg ttgccataac agatcctcta aaactagtgt tgagcacgtt cagaacgagc
     3661 ccagaaattt ggttagacgg tttcggcgac gggtgtcgcc gaagtgagcc gtcttaggaa
     3721 tatctgactt aggcttgtgg cgccttaggc agaatgtttt ctacttctga gtggacacgc
     3781 gcaatgatgt gcgcagcaac caagccatca ccaacacgtt cgcaagcatc ggcgccagca
     3841 cgaacagcgg cgttaacagc gcctgtttcg ccccgaacca atacggtgac gtaaccgcca
     3901 ccaacaaatt gacgaccgac caaacgcact tcagccgctt tagtcatggc gtccgctgct
     3961 tcaatcgcag gaactaagcc acgtgtttcg atcataccca gagcaatacc agttacatca
     4021 gccatttaaa taaactccta agctgtcact ttatgcaaag aaccggaaca agcctgcgcc
     4081 ggttcgtctt tcccaatcct taagcttcag gggctttcgg caggatgttt tcgacttcgg
     4141 aatggacacg ggcaatgata tgcgccgcga ccagaccatc gccgactcgt tcgcaggcat
     4201 cagcgcccgc acgaactgct gcgttgacgg caccggtttc accacggacc aaaacggtca
     4261 cgtaaccacc accaacaaat tgacggccaa ccaaacgtac ttcggcggcc ttggtcatgg
     4321 catcggcagc ttcaatcgct ggaaccagac cacgtgtttc aatcataccc agtgcaatac
     4381 ctgttactgc tgccatttct tctctcctca ataaagactc attcaaaatc aactcatcta
     4441 gcgatggtca agttacccag tgatcgatga tgccaccaat cgtcaaatcc gtgagaatct
     4501 caaaatcacc cacgccaaac cgagcggcag agccgctaat cgtaaagacc caacatccct
     4561 cgggaacgcc aatgggatcg caagcgacac tgaccttacc cgtagcatcc tccatgacac
     4621 gcaaagagat atttttaaga ccgggaatcc tgcgtgttgc gattaggtcg gaacgaacgc
     4681 gcattacttc cattactcac cattccactg atcaataatc ccgataatcg tcaaatcaga
     4741 gggataagac ttgcttcctg cagcctctcg tgctgccgat gacccaacgc acaaaaccca
     4801 atcgcccggt atgcagccaa tcgcatccac ggcgacctgc ctgggcgcgc ccggtttctc
     4861 ccataccact aatagtggtt tgtgacccat atcagcaata cggtttgttg aaaccaacgt
     4921 tttctcaact tgcatgattt tcatgcggat gcaacctctt caatcgggct tccgatatcc
     4981 tgcgcctgaa cgctcaaacg aaactgcaat agattctgct catccaagga tgagaaccgc
     5041 tctttaatgg cgttgtatat ccgtcttgct ttgaccacgg ttctttctct ggagcctgga
     5101 acattggggt catagcgata gtggatggca atcggaatcg gtaaacctcg actcaaatta
     5161 agtttggtga aaattttgat tcccacatcc acatcaatcg cattttcttc aaccgtatcc
     5221 aaatgcgcgt agtaggctaa attccggatt tgaacttcat caaaaccatc accaacactg
     5281 atatatcgct cagcatgacc aatatcattg ggtggatagc gaccaccatg acgatttact
     5341 acgtaatcga tttgcgagag gttgttgatc aaaagctgac caataaaacg acgcatcccc
     5401 tcgctggcta ctccatttcc tttagcccaa ccatcgctgc ggtttgcgtt gagaattgct
     5461 tcgtgaataa tcacacgagc ctcatccggc gccagatgta gtgtttgcgc ataagttact
     5521 gtgttgtcaa cataacgata cggattcaaa aaacctttgc tatccggaat atgaacgcga
     5581 atggcatccg tatccgtatc aacgccaatc agtaaaatat cgatacttgc gccgcagcaa
     5641 tgggcatttt caaccgcttc tctgaatttc atcagctggg ttaaagccgc ttccagtgct
     5701 gcacgatcat tactgccgtg tgccgcgcag ccagagtgtg tcgggtcaga ggtgctgaaa
     5761 tgatacacag caattttcag gtatcgtgtt ggctgatccg ctgtatttgg aacaccttca
     5821 cgataacggc ggagttcatt tttctcccac tgtgccagat catcttcaat atcgaacatc
     5881 gagcctgcgt aggctttacg ataggtgaat gccgtaaggg gcaaacgcaa tatgtaaggc
     5941 agtaaccctt tgagacgacc atcggcacaa gggcttatgt ccactgcgtg gaacccgcaa
     6001 tccaggaaga agccaatggc cgatgcggaa tcgtccttct cagacattat tttacgactg
     6061 aattgttccg tggctgctgt tagtgcttta gcagtcgcgt agccaaaaat gccttttaaa
     6121 ttgtgcccac tgatccaatt atctttgagt aattcttcgg gcagcgtatg tccaagcgac
     6181 gactgggcta attcttgaac acgggtaaca taatccagac caagaggaat gccagcaaca
     6241 acttgcaaga aagggataac gcgatcaaaa cggttcttga cgctatcctc gcaacgaccc
     6301 agttgttcgt tggcttcaag gtcagttaac gggtgccggc aggtacgctc aggcaacgtt
     6361 atgcaagcag gatggcggtc ataggcaggg tttggtgctt gctcaatcaa gtcaagccga
     6421 ggtttgactg atgagctaac accaaacggt gcgcgttgct tgcttcgtgt gttacgggtg
     6481 ttcatgatcg ttacacttta cttaatcaac cgcgcgcgcc gccggagtaa gtgatcagac
     6541 taccttgggt gtcattacca ctactgcccg tgatcttgga gcccggcttt tcaggttttg
     6601 gcacattgcg attggcaaac gcgctggttt cgaccacacg cgcattaccg cgcatggaag
     6661 ggttgcgacc actcgcccat tgaccggctg taccggtgat gtgcttgtta actgaccagt
     6721 catcgccagt gatgtggcaa cgttctttgc cttcgccact cacacgatcc atttgatcgc
     6781 ttcttggtgc acataccaac tcaggctttt cctgagttgc aaccacttct ggctgcacaa
     6841 caggtgcttg agcagcctta ttacggttgg ccatggccat tgccatgggt tgtggagaac
     6901 caaccaactc gcgcgcagcg tgcctgaatt cgggggttcc tgtaatcaag ccggttgcca
     6961 gcataccagg gcctgtaatg cgacctgagg gcgcaatgtc gttacctgta atgcgattct
     7021 gagccgagcg agctggggtt tgaatactaa agtcagtagg cattacttca cctggtgcaa
     7081 agttttgttc tggctgattg gtgttcttaa aacccattga ctgaacattg ccagtttggt
     7141 tgaagcgtgg actagtggga aggcaacctg tctgctgcgc gccaacatag gcgtcaccgc
     7201 tgatgagttg ctgttcaccg atttcatttc ctgtgaccaa atcactggcg tcaactgaag
     7261 tgccgctaat aatttgtcct tcacaggtca atgattgttc agttgattga gcttcgggct
     7321 ctggggtgct cgtacaaaac ggttcgaaat gttcacgacc gtagtactca ttaccggtga
     7381 cgggcatgca cccgccgcgc tcgtcaccgg acatctttgg ggcatgatct agctgttggc
     7441 cagatactga ggtgccgcga agggtgcgca ttgagcgcac ttttcccacg ccaccaccgc
     7501 agattttgct ttggccatac tgagagcctg taacccgaga gcatgaacct ggttcgttac
     7561 ctgtaaccag ttcggaacga tcaaccaagt taccagtcac agactgaccc ttgttcgtgc
     7621 gatcttggcc aaccttgaac gggctgcgtt gaggttttgt gtcacaaaag gattggaatt
     7681 gctcgttact gagatactcg gtgcctgaga ttgaacgaca cgaaccgctt tcatcaccag
     7741 ttacgcgagt agagcgaccg atttccgtgc cggtaacgtc agagcccgca aaggtgtggg
     7801 ttctggctac ttttgcaggt gctccgttga tcgtgagtcg cgcaagacct tcgtctgcgt
     7861 actgcgagcc ggtaattgtg cgttttgcac ccgattcgtt acccgtaaca gaagagggcc
     7921 tgaattcatc actgccactg accacttgat gcgttcgtgc tgtggcgccg aacatgactt
     7981 tatccgcttg tgaaggcttt gcggctgttc cacatagaga aaagtgagct tcattactca
     8041 tgtattcggt gccggtaacg ttacggcaga caccggattc gttgccagtt accttctcgg
     8101 tccgtgaaac ttccgtaccg ctaacagggc gaccacgagc cgttgtggtc acattgacct
     8161 tcgtcgcatt tggcttgggg ctggtattgc aaaaagaggt gaattgctca gtacctacgt
     8221 actcggtgcc cgtgactgct cggcaagtgc ccggctcgtt tccagtcaca tgagatttac
     8281 gatccacttg cgtgcccgta acagatccgc cggatagggt gtgaccggtt tcaacctttt
     8341 ttggcgctgc gttgcccgac tgtacagaat ttttgacacg gcctgttggc ttgcaggaag
     8401 gtgctgtgcc gcggccgtat tgacagagca tttcacggtg gcgcttggcg gcatcgcggc
     8461 catgaagtgc ggtatctagg tatccatcaa tttgatagcc attgcgtcct ggaccacctt
     8521 gagattgcgg ctttggcggc acggcgcgag ttccacgctc agagagcgct tgacggcgtg
     8581 cacggcagat atcacggacg gttttggctt gtgaaccaaa acgctccggc atgtcgtctg
     8641 ctatggagca gatttcggaa agttcattat ttaatgaagt ggacgatgat tggttagtat
     8701 caccggcatt ttcgatcatt gcgtcgttgt gaaccactga ttgctgtggt cgaacgcgac
     8761 ccgacggctt gccgttggtt ttaaccgctt ttttaccgat agttgatagt gcctctctgc
     8821 gttgacggca gagatctcta acgcgattgc tttcggtacc aaagtcagca tgatcaatcg
     8881 tagagcacaa ttgatccgcc atttggtaat cagtgggcac ttcatttaca gaagagcgca
     8941 cctgatttgt attgatcgaa gatgccgcct gtttagtgct acggtttacg ctagccgttt
     9001 tattagaaac agctgctttc ccttgcttgg atagtgcagc gcggcgtgcg cgtgccagtt
     9061 ccttgccaga gagtccgctc aggtcggcag gattcattcc tgactgtgaa ggcatgtttg
     9121 accccatctt gaataagtgc tgtgcgtaga gaaacgcaca gcgcaatgac agacttgact
     9181 tagttgccgc ggtagaccac gaaggccaga cctaagcttt gcgcatagtt gtcataagcc
     9241 accagtttga cctggtgtgt tggatacgca ctacgacacg cttcaatttc agccaacacg
     9301 ttgtcgacat tttgttcgcc gaagaaggga agtttccaca tgtaccaata ttggttcatg
     9361 gagtttttca cttctacgtg ctcaatgccg gggctccagc cttgagcaat tgcgtacttg
     9421 atttgagcgc ggatgcgttc cgcgttcatg ggtggaagat aagagaaagt ctcatatttg
     9481 aggctttgct tgtaatcctg catttcagcc atggttactc accttagtat gttgtgtggt
     9541 acgagggatc aacgattttg agtgtcgagt ttgtcgacag tgtcaaattc gaatttgatc
     9601 tctttccaag tttccatggc aatcttcagt tctgggctgt gctgtgcagc agcagtcaga
     9661 atttctttgc cttctttttc gatatcgcgg ccttggttac gcgcttctac gcaggcttcc
     9721 agagcaacac ggttggcagc agcaccggca gcgttgcccc atggatgacc cagcgtaccg
     9781 ccaccgaatt ggaggacaga gtcgtcacca aagatgttaa ccagcgcagg catgtgccat
     9841 acgtgaatac caccagaggc cacagcgaat acgcctggca ttgaacccca gtcttgatcg
     9901 aagaagatac cgcgcgagcg atcttcaggg ataaacgatt cgcggagcaa atcaatccag
     9961 cccagagtag aggcacggtc gccttccagt ttgccgacaa cggtaccggt gtgcaggtga
    10021 tcgccacccg acaaacgcag aatcttggtc agaacacgga agtgaatacc gtggttcggg
    10081 ttacggtcga taaccgcatg catcgcacgg tggatgtgca gcagtacgcc gttgtcttga
    10141 caccacttgg ccaagccagt gttggccgtg aagccaccgg tgatgtagtc gtgcataatg
    10201 attggcgcgc caatttcttt ggcgaattct gcgcgcttgt acatttcttc aggagttggc
    10261 gccgttacgt tgaggtaatg gcctttgcgt tcgccggttt gggcttcagc agtttcggtc
    10321 gcgtcttgta cgaacaagaa gcgatcgcgc cagcgcatga acggctgaga attgatgttt
    10381 tcatcatctt tagtgaagtc gaggccgcca cggaggcact cgtatacggc acggccgtag
    10441 tttttcgcag acaaaccaag ttttggcttg atggtgcaac ccaacagtgg gcgaccatat
    10501 ttgttcatct tgtcgcgttc gacttgaata ccgtgcggtg ggccgccaca ggttttaacg
    10561 taggcgagtg ggaagcgaac atcttccaga cgcaggccgc gtaccgcttt gaagccgaac
    10621 acgttaccaa ccagtgaggt aaacacgtta acaactgacc cttcttcgaa caggtcgatt
    10681 gggtaggcga taaaggcata gaatgccgca tcgtcaccgg gtacgtcttc aatgcgatag
    10741 gcacggcctt tgtagcggtc catgtcggtc agcaaatcgg tccacacggt ggtccatgtg
    10801 ccggttgaag attctgctgc aaccgcggct gcggcttctt cgcgatcaac acccggttgt
    10861 ggggtgattt tgaagcatgc aaggatgtcg gaatccaacg gtgtgtattc cggcatccaa
    10921 taggtctgcc ggtattcttt tacaccagca ctatactttt taactgccat ggtacctttc
    10981 tcctctttaa tgaattcggt cagtgcgtcc tgctgatgtg ctcagtatat ctatcactga
    11041 tagggatgtc aatctctatc actgataggg actcgaggtg aagacgaaag ggcctcgtga
    11101 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcggaattgc
    11161 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
    11221 tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat
    11281 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
    11341 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
    11401 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
    11461 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
    11521 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
    11581 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
    11641 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
    11701 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
    11761 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
    11821 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
    11881 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
    11941 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
    12001 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
    12061 gccttcttga cgagttcttc tgagcgggac tctggggttc gagagctcgc ttggactcct
    12121 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
    12181 ccgccgggcg ttttttattg gtgagaatcc aagcactagt catgaccaaa atcccttaac
    12241 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    12301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    12361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    12421 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
    12481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    12541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    12601 agcggtcggg ctgaacg
//
