LOCUS       sequence-327003-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-fragment of the DiB2‐split protein. This plasmid needs to be 
            co-transformed with Plasmid 168257: pMRBad-C-wtBlc to obtain 
            DiB2‐split protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Bozhanova NG, Gavrikov AS, Mishin AS, Meiler J
  TITLE     DiB-splits: nature-guided design of a novel fluorescent labeling 
            split system.
  JOURNAL   Sci Rep. 2020 Jul 6;10(1):11049. doi: 10.1038/s41598-020-67095-2.
  PUBMED    32632329
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-07-6"; volume: "10(1):11049. doi"; pages: " 
            10.1038/s41598-020-67095-2"
COMMENT     Sequence Label: pET11a-N-DiB2
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(408..426)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      422..469
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(644..649,650..666,667..672))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(699..717)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatggggc atcaccacca ccatcacctc
       61 gaatcaacca gcttgtacaa gaaatccagc tccacgccgc cgcgtggcgt gaccgtagta
      121 aataatttcg actgcaaacg ttatctcggt acctggtatg agattgcccg ttttgatcac
      181 cgctttgaac gtggactgga aaaagtcacc gcaacataca gcctgcgtga tgacggcggc
      241 ctgaatgtca ttaataaagg ctataaccct gacagaggaa tgtggcagca gagtgaaggg
      301 aaagcgtact ttaccggcgc accaactcgc gctgcgctga aagtgtcatt cttttaagga
      361 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      421 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      481 aactatatcc ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct
      541 acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac
      601 ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg
      661 ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt
      721 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
      781 tgtgcgcgga a
//
