LOCUS       sequence-327005-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be 
            co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain 
            DiB2‐split-Zip protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Bozhanova NG, Gavrikov AS, Mishin AS, Meiler J
  TITLE     DiB-splits: nature-guided design of a novel fluorescent labeling 
            split system.
  JOURNAL   Sci Rep. 2020 Jul 6;10(1):11049. doi: 10.1038/s41598-020-67095-2.
  PUBMED    32632329
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-07-6"; volume: "10(1):11049. doi"; pages: " 
            10.1038/s41598-020-67095-2"
COMMENT     Sequence Label: pET11a-Z-N-DiB2
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             429..440
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(518..536)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      532..579
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(754..759,760..776,777..782))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(809..827)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatggggca tcaccaccac catcacctcg
       61 aatcaaccag cttgtacaag aaatccagct ccacgccgcc gcgtggcgtg accgtagtaa
      121 ataatttcga ctgcaaacgt tatctcggta cctggtatga gattgcccgt tttgatcacc
      181 gctttgaacg tggactggaa aaagtcaccg caacatacag cctgcgtgat gacggcggcc
      241 tgaatgtcat taataaaggc tataaccctg acagaggaat gtggcagcag agtgaaggga
      301 aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtggct
      361 ctggctctgg ctcgagcgcc ctcaaaaaag aattgcaggc aaacaaaaaa gaacttgcgc
      421 agctgaagtg ggagttacaa gctctgaaaa aggaactggc gcagtaagga tccggctgct
      481 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      541 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      601 ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
      661 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
      721 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc
      781 aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ata
//
