LOCUS       sequence-327016-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be
            co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain 
            DiB-RM‐split-Zip protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Bozhanova NG, Harp JM, Bender BJ, Gavrikov AS, Gorbachev DA, Baranov
            MS, Mercado CB, Zhang X, Lukyanov KA, Mishin AS, Meiler J
  TITLE     Computational redesign of a fluorogen activating protein with 
            Rosetta.
  JOURNAL   PLoS Comput Biol. 2021 Nov 8;17(11):e1009555. doi: 
            10.1371/journal.pcbi.1009555. eCollection 2021 Nov.
  PUBMED    34748541
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2021-11-8"; volume: "17(11):e1009555. doi"; pages: " 
            10.1371/journal.pcbi.1009555. eCollection 2021 Nov"
COMMENT     Sequence Label: pMRBad-Z-C-DiB-RM
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        125..409
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     332..351
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             436..458
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag
       61 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc
      121 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg
      181 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
      241 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac
      301 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta
      361 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt
      421 tgggctagaa ataattttgt ttaactttaa gaaggagata tacccatggc aagcgagcag
      481 ctggaaaaga agttacaagc cctggagaaa aaacttgctc agctggaatg gaaaaaccaa
      541 gcattggaaa aaaaactcgc gcagacgtcg ggtggaagcg gtggtccttt ctatggcggt
      601 tataacgtta ttgcactcga tcgggaatac cgccatgcgc tggtttgcgg cccggaccgc
      661 gactacctgt ggatcaactc ccgcacgcca accatttctg acgaagtgaa acaggagatg
      721 ctggcagtcg cgacccggga agggtttgat gtcagtaaat ttatttgggt acagcagcct
      781 ggtagttagt gtacaactaa ggatccggct gctaacaaag cccgaaag
//
