LOCUS       sequence-327018-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-fragment of the DiB-RM‐split protein. This plasmid needs to be 
            co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain 
            DiB-RM‐split protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Bozhanova NG, Harp JM, Bender BJ, Gavrikov AS, Gorbachev DA, Baranov
            MS, Mercado CB, Zhang X, Lukyanov KA, Mishin AS, Meiler J
  TITLE     Computational redesign of a fluorogen activating protein with 
            Rosetta.
  JOURNAL   PLoS Comput Biol. 2021 Nov 8;17(11):e1009555. doi: 
            10.1371/journal.pcbi.1009555. eCollection 2021 Nov.
  PUBMED    34748541
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2021-11-8"; volume: "17(11):e1009555. doi"; pages: " 
            10.1371/journal.pcbi.1009555. eCollection 2021 Nov"
COMMENT     Sequence Label: pMRBad-C-DiB-RM
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             256..278
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(305..589)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(363..382)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcctta gttgtacact aactaccagg ctgctgtacc
       61 caaataaatt tactgacatc aaacccttcc cgggtcgcga ctgccagcat ctcctgtttc
      121 acttcgtcag aaatggttgg cgtgcgggag ttgatccaca ggtagtcgcg gtccgggccg
      181 caaaccagcg catggcggta ttcccgatcg agtgcaataa cgttataacc gccatagaaa
      241 ggacccatgg gtatatctcc ttcttaaagt taaacaaaat tatttctagc ccaaaaaaac
      301 gggtatggag aaacagtaga gagttgcgat aaaaagcgtc aggtaggatc cgctaatctt
      361 atggataaaa atgctatggc atagcaaagt gtgacgccgt gcaaataatc aatgtggact
      421 tttctgccgt gattatagac acttttgtta cgcgtttttg tcatggcttt ggtcccgctt
      481 tgttacagaa tgcttttaat aagcggggtt accggtttgg ttagcgagaa gagccagtaa
      541 aagacgcagt gacggcaatg tctgatgcaa tatggacaat tggtttcttc tctgaatggc
      601 gggagtatga aaagtatggc tgaagcgcaa aatgatcccc tgctgccggg atactcgttt
      661 aatgcccatc tggtggcggg tttaacgccg attgaggcca acggttatct cgattttttt
      721 atcgaccgac cgctgggaat gaaaggttat attctcaatc tcaccattcg cggtcagggg
      781 gtggtgaaaa atcagggacg agaatttgtt tgccgaccgg g
//
