LOCUS       sequence-327020-        6306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mEos3.2 in Mycobacterium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Rashid FM, Mahlandt E, van der Vaart M, Boer DEC, Varela Alvarez M, 
            Henneman B, Brocken DJW, Voskamp P, Blok AJ, Shimizu TS, Meijer AH, 
            Luijsterburg MS, Goedhart J, Cremazy FGE, Dame RT
  TITLE     HI-NESS: a family of genetically encoded DNA labels based on a 
            bacterial nucleoid-associated protein.
  JOURNAL   Nucleic Acids Res. 2021 Nov 4. pii: 6414584. doi: 
            10.1093/nar/gkab993.
  PUBMED    34734265
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Nov 4. pii: 6414584. doi: 10.1093/nar/gkab993."
COMMENT     Sequence Label: pRD445
FEATURES             Location/Qualifiers
     source          1..6306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..49)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(34..50)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             104..784
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     primer_bind     complement(913..932)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(997..1016)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3107..3695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3596..3615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5758..5774
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5758..5774
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc
       61 ggtaccagat ctttaaatct agatttaaga aggagatata catatgagtg cgattaagcc
      121 agacatgaag atcaaactcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga
      181 cggagatggt acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga
      241 gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag
      301 ggtattcgcc aaatatccag acaacataca agactatttt aagcagtcgt ttcctaaggg
      361 gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcaacg ccagaaacga
      421 cataacaatg gaaggggaca ctttctataa taaagttcga ttttatggta ccaactttcc
      481 cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat
      541 gtatgtgcgt gatggagtgc tgacgggtga tattgagatg gctttgttgc ttgaaggaaa
      601 tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt
      661 accaggcgcc cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa
      721 caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgcaagacg
      781 ataggctagc cacctgacgt cggggggggg gggaaagcca cgttgtgtct caaaatctct
      841 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
      901 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc
      961 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1021 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gccccatgcg ccagagttgt
     1081 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1141 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1201 actagagcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1261 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1321 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1381 ccgacgtggc cgaccagccc gtcatcgtca acgcctgatc cgcggtgcgg acaggccgtg
     1441 tcgcgaccgg ccgtgcggaa ttaagccggc ccgtaccctg tgaatagagg tccgctgtga
     1501 cacaagaatc cctgttactt ctcgaccgta ttgattcgga tgattcctac gcgagcctgc
     1561 ggaacgacca ggaattctgg gagccgctgg cccgccgagc cctggaggag ctcgggctgc
     1621 cggtgccgcc ggtgctgcgg gtgcccggcg agagcaccaa ccccgtactg gtcggcgagc
     1681 ccggcccggt gatcaagctg ttcggcgagc actggtgcgg tccggagagc ctcgcgtcgg
     1741 agtcggaggc gtacgcggtc ctggcggacg ccccggtgcc ggtgccccgc ctcctcggcc
     1801 gcggcgagct gcggcccggc accggagcct ggccgtggcc ctacctggtg atgagccgga
     1861 tgaccggcac cacctggcgg tccgcgatgg acggcacgac cgaccggaac gcgctgctcg
     1921 ccctggcccg cgaactcggc cgggtgctcg gccggctgca cagggtgccg ctgaccggga
     1981 acaccgtgct caccccccat tccgaggtct tcccggaact gctgcgggaa cgccgcgcgg
     2041 cgaccgtcga ggaccaccgc gggtggggct acctctcgcc ccggctgctg gaccgcctgg
     2101 aggactggct gccggacgtg gacacgctgc tggccggccg cgaaccccgg ttcgtccacg
     2161 gcgacctgca cgggaccaac atcttcgtgg acctggccgc gaccgaggtc accgggatcg
     2221 tcgacttcac cgacgtctat gcgggagact cccgctacag cctggtgcaa ctgcatctca
     2281 acgccttccg gggcgaccgc gagatcctgg ccgcgctgct cgacggggcg cagtggaagc
     2341 ggaccgagga cttcgcccgc gaactgctcg ccttcacctt cctgcacgac ttcgaggtgt
     2401 tcgaggagac cccgctggat ctctccggct tcaccgatcc ggaggaactg gcgcagttcc
     2461 tctgggggcc gccggacacc gcccccggcg cctgacgccc cctctagtaa tcttttgcca
     2521 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     2581 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     2641 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2701 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2761 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2821 acttgacggg acggcggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
     2881 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
     2941 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
     3001 atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcact
     3061 agttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3241 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3481 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3721 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3781 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3841 ccgagcgcaa cgcgtgagcc caccagctcc gtaagttcgg gtgctgtgtg gctcgtaccc
     3901 gcgcattcag gcggcagggg gtctaacggg tctaaggcgg cgtgtacggc cgccacagcg
     3961 gctcttagcg gcccggaaac gtcctcgaaa cgacgcatgt gttcctcctg gttggtacag
     4021 gtggttgggg gtgctcggct gtcgctggtg tttcatcatc agggctcgac gggagagcgg
     4081 gggagtgtgc agttgtgggg tggcccctca gcgaaatatc tgacttggag ctcgtgtcgg
     4141 accatacacc ggtgattaat cgtggtttat tatcaagcgt gagccacgtc gccgacgaat
     4201 ttgagcagct ctggctgccg tactggtccc tggcaagcga cgatctgctc gaggggatct
     4261 accgccaaag ccgcgcgtcg gccctaggcc gccggtacat cgaggcgaac ccaacagcgc
     4321 tggcaaacct gctggtcgtg gacgtagacc atccagacgc agcgctccga gcgctcagcg
     4381 cccgggggtc ccatccgctg cccaacgcga tcgtgggcaa tcgcgccaac ggccacgcac
     4441 acgcagtgtg ggcactcaac gcccctgttc cacgcaccga atacgcgcgg cgtaagccgc
     4501 tcgcatacat ggcggcgtgc gccgaaggcc ttcggcgcgc cgtcgatggc gaccgcagtt
     4561 actcaggcct catgaccaaa aaccccggcc acatcgcctg ggaaacggaa tggctccact
     4621 cagatctcta cacactcagc cacatcgagg ccgagctcgg cgcgaacatg ccaccgccgc
     4681 gctggcgtca gcagaccacg tacaaagcgg ctccgacgcc gctagggcgg aattgcgcac
     4741 tgttcgattc cgtcaggttg tgggcctatc ttcccgccct catgcggatc tacctgccga
     4801 cccggaacgt ggacggactc ggccgcgcga tctatgccga gtgccacgcg cgaaacgccg
     4861 aatttccgtg caacgacgtg tgtcccggac cgctaccgga cagcgaggtc cgcgccatcg
     4921 ccaacagcat ttggcgttgg atcacaacca agtcgcgcat ttgggcggac gggatcgtgg
     4981 tctacgaggc cacactcagt gcgcgccatg cggccatctc gcggaagggc gcagcagcgc
     5041 gcacggcggc gagcacagtt gcgcggcgcg caaagtccgc gtcagccatg gaggcattgc
     5101 tatgagcgac ggctacagcg acggctacag cgacggctac aactggcagc cgactgtccg
     5161 caaaaagcgg cgcgtgaccg ccgccgaagg cgctcgaatc accggactat ccgaacgcca
     5221 cgtcgtccgg ctcgtggcgc aggaacgcag cgagtggttc gccgagcagg ctgcacgccg
     5281 cgaacgcatc cgcgcctatc acgacgacga gggccactct tggccgcaaa cggccaaaca
     5341 tttcgggctg catctggaca ccgttaagcg actcggctat cgggcgagga aagagcgtgc
     5401 ggcagaacag gaagcggctc aaaaggccca caacgaagcc gacaatccac cgctgttcta
     5461 acgcaattgg ggagcgggtg tcgcgggggt tccgtggggg gttccgttgc aacgggtcgg
     5521 acaggtaaaa gtcctggtag acgctagttt tctggtttgg gccatgcctg tctcgttgcg
     5581 tgtttcgttg cgtccgtttt gaataccagc cagacgagac ggggttctac gaatcttggt
     5641 cgataccaag ccatttccgc tgaatatcgt ggagctcacc gccagaatcg gtggttgtgg
     5701 tgatgtacgt ggcgaactcc gttgtagtgc ttgtggtggc atccgtggcg cggccgctct
     5761 agaactagtg gatctgaccc gctccacaac cgctgagcag ggtggcagtc agagcgacca
     5821 ggccgaccga tacgaggcgg attttgaaca ggttcactcg aagcctcccg ctgggttcgg
     5881 agcatcgtcg atcacatgac ggcgtgctga caccgattcg actatgcaca gtagtaggta
     5941 gccgccgcgg acggtagcgc aggcggcctc ggcgccgtcg tggcgtcgcg atggttggcg
     6001 ccagacccgc ctgatcggcg tcccgaccag ggccgccggg gtggtgagcc tcggcttggt
     6061 ccagccgtag tgagacggga tgtcttcccg gttgtcgggt ttgggtgggc ggtcacatgt
     6121 tcgctggctg tgggcgtgtc gggggtcgtc gaagcggccg cgaacagggc tggacgatgc
     6181 acgtcttgca cctcctgtca acccccaatg ttgagccgcg atgcccctga cctgcaccgt
     6241 tggaccgcag tcgttagggg ggcgtgttag catggagtga cgaaaggggc ctaaatcaga
     6301 tgatcc
//
