LOCUS       sequence-327021-        6480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mEos3.2_H-NSdbd in Mycobacterium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Rashid FM, Mahlandt E, van der Vaart M, Boer DEC, Varela Alvarez M, 
            Henneman B, Brocken DJW, Voskamp P, Blok AJ, Shimizu TS, Meijer AH, 
            Luijsterburg MS, Goedhart J, Cremazy FGE, Dame RT
  TITLE     HI-NESS: a family of genetically encoded DNA labels based on a 
            bacterial nucleoid-associated protein.
  JOURNAL   Nucleic Acids Res. 2021 Nov 4. pii: 6414584. doi: 
            10.1093/nar/gkab993.
  PUBMED    34734265
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Nov 4. pii: 6414584. doi: 10.1093/nar/gkab993."
COMMENT     Sequence Label: pRD393
FEATURES             Location/Qualifiers
     source          1..6480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..49)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(34..50)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             104..781
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     primer_bind     complement(1087..1106)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1171..1190)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3281..3869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3770..3789
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5932..5948
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5932..5948
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc
       61 ggtaccagat ctttaaatct agatttaaga aggagatata catatgagtg cgattaagcc
      121 agacatgaag atcaaactcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga
      181 cggagatggt acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga
      241 gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag
      301 ggtattcgcc aaatatccag acaacataca agactatttt aagcagtcgt ttcctaaggg
      361 gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcaacg ccagaaacga
      421 cataacaatg gaaggggaca ctttctataa taaagttcga ttttatggta ccaactttcc
      481 cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat
      541 gtatgtgcgt gatggagtgc tgacgggtga tattgagatg gctttgttgc ttgaaggaaa
      601 tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt
      661 accaggcgcc cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa
      721 caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgcaagacg
      781 agctgccgtt aaatctggca ccaaagctaa acgtgctcag cgtccggcaa aatatagcta
      841 cgttgacgaa aacggcgaaa ctaaaacctg gactggccaa ggccgtactc cagctgtaat
      901 caaaaaagca atggatgagc aaggtaaatc cctcgacgat ttcctgatca agcaataggc
      961 tagccacctg acgtcggggg gggggggaaa gccacgttgt gtctcaaaat ctctgatgtt
     1021 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     1081 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat
     1141 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     1201 aatcaggtgc gacaatctat cgcttgtatg ggaagcccca tgcgccagag ttgtttctga
     1261 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     1321 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgactaga
     1381 gcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1441 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1501 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgacg
     1561 tggccgacca gcccgtcatc gtcaacgcct gatccgcggt gcggacaggc cgtgtcgcga
     1621 ccggccgtgc ggaattaagc cggcccgtac cctgtgaata gaggtccgct gtgacacaag
     1681 aatccctgtt acttctcgac cgtattgatt cggatgattc ctacgcgagc ctgcggaacg
     1741 accaggaatt ctgggagccg ctggcccgcc gagccctgga ggagctcggg ctgccggtgc
     1801 cgccggtgct gcgggtgccc ggcgagagca ccaaccccgt actggtcggc gagcccggcc
     1861 cggtgatcaa gctgttcggc gagcactggt gcggtccgga gagcctcgcg tcggagtcgg
     1921 aggcgtacgc ggtcctggcg gacgccccgg tgccggtgcc ccgcctcctc ggccgcggcg
     1981 agctgcggcc cggcaccgga gcctggccgt ggccctacct ggtgatgagc cggatgaccg
     2041 gcaccacctg gcggtccgcg atggacggca cgaccgaccg gaacgcgctg ctcgccctgg
     2101 cccgcgaact cggccgggtg ctcggccggc tgcacagggt gccgctgacc gggaacaccg
     2161 tgctcacccc ccattccgag gtcttcccgg aactgctgcg ggaacgccgc gcggcgaccg
     2221 tcgaggacca ccgcgggtgg ggctacctct cgccccggct gctggaccgc ctggaggact
     2281 ggctgccgga cgtggacacg ctgctggccg gccgcgaacc ccggttcgtc cacggcgacc
     2341 tgcacgggac caacatcttc gtggacctgg ccgcgaccga ggtcaccggg atcgtcgact
     2401 tcaccgacgt ctatgcggga gactcccgct acagcctggt gcaactgcat ctcaacgcct
     2461 tccggggcga ccgcgagatc ctggccgcgc tgctcgacgg ggcgcagtgg aagcggaccg
     2521 aggacttcgc ccgcgaactg ctcgccttca ccttcctgca cgacttcgag gtgttcgagg
     2581 agaccccgct ggatctctcc ggcttcaccg atccggagga actggcgcag ttcctctggg
     2641 ggccgccgga caccgccccc ggcgcctgac gccccctcta gtaatctttt gccattctca
     2701 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2761 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2821 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2881 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2941 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     3001 cgggacggcg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc
     3061 atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt
     3121 ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg
     3181 cgattcaggc ctggtatgag tcagcaacac cttcttcacg aggcagacct cactagttcc
     3241 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3301 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3361 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3421 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3481 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3541 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3601 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3661 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3721 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3781 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3841 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3901 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3961 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4021 gcaacgcgtg agcccaccag ctccgtaagt tcgggtgctg tgtggctcgt acccgcgcat
     4081 tcaggcggca gggggtctaa cgggtctaag gcggcgtgta cggccgccac agcggctctt
     4141 agcggcccgg aaacgtcctc gaaacgacgc atgtgttcct cctggttggt acaggtggtt
     4201 gggggtgctc ggctgtcgct ggtgtttcat catcagggct cgacgggaga gcgggggagt
     4261 gtgcagttgt ggggtggccc ctcagcgaaa tatctgactt ggagctcgtg tcggaccata
     4321 caccggtgat taatcgtggt ttattatcaa gcgtgagcca cgtcgccgac gaatttgagc
     4381 agctctggct gccgtactgg tccctggcaa gcgacgatct gctcgagggg atctaccgcc
     4441 aaagccgcgc gtcggcccta ggccgccggt acatcgaggc gaacccaaca gcgctggcaa
     4501 acctgctggt cgtggacgta gaccatccag acgcagcgct ccgagcgctc agcgcccggg
     4561 ggtcccatcc gctgcccaac gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag
     4621 tgtgggcact caacgcccct gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat
     4681 acatggcggc gtgcgccgaa ggccttcggc gcgccgtcga tggcgaccgc agttactcag
     4741 gcctcatgac caaaaacccc ggccacatcg cctgggaaac ggaatggctc cactcagatc
     4801 tctacacact cagccacatc gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc
     4861 gtcagcagac cacgtacaaa gcggctccga cgccgctagg gcggaattgc gcactgttcg
     4921 attccgtcag gttgtgggcc tatcttcccg ccctcatgcg gatctacctg ccgacccgga
     4981 acgtggacgg actcggccgc gcgatctatg ccgagtgcca cgcgcgaaac gccgaatttc
     5041 cgtgcaacga cgtgtgtccc ggaccgctac cggacagcga ggtccgcgcc atcgccaaca
     5101 gcatttggcg ttggatcaca accaagtcgc gcatttgggc ggacgggatc gtggtctacg
     5161 aggccacact cagtgcgcgc catgcggcca tctcgcggaa gggcgcagca gcgcgcacgg
     5221 cggcgagcac agttgcgcgg cgcgcaaagt ccgcgtcagc catggaggca ttgctatgag
     5281 cgacggctac agcgacggct acagcgacgg ctacaactgg cagccgactg tccgcaaaaa
     5341 gcggcgcgtg accgccgccg aaggcgctcg aatcaccgga ctatccgaac gccacgtcgt
     5401 ccggctcgtg gcgcaggaac gcagcgagtg gttcgccgag caggctgcac gccgcgaacg
     5461 catccgcgcc tatcacgacg acgagggcca ctcttggccg caaacggcca aacatttcgg
     5521 gctgcatctg gacaccgtta agcgactcgg ctatcgggcg aggaaagagc gtgcggcaga
     5581 acaggaagcg gctcaaaagg cccacaacga agccgacaat ccaccgctgt tctaacgcaa
     5641 ttggggagcg ggtgtcgcgg gggttccgtg gggggttccg ttgcaacggg tcggacaggt
     5701 aaaagtcctg gtagacgcta gttttctggt ttgggccatg cctgtctcgt tgcgtgtttc
     5761 gttgcgtccg ttttgaatac cagccagacg agacggggtt ctacgaatct tggtcgatac
     5821 caagccattt ccgctgaata tcgtggagct caccgccaga atcggtggtt gtggtgatgt
     5881 acgtggcgaa ctccgttgta gtgcttgtgg tggcatccgt ggcgcggccg ctctagaact
     5941 agtggatctg acccgctcca caaccgctga gcagggtggc agtcagagcg accaggccga
     6001 ccgatacgag gcggattttg aacaggttca ctcgaagcct cccgctgggt tcggagcatc
     6061 gtcgatcaca tgacggcgtg ctgacaccga ttcgactatg cacagtagta ggtagccgcc
     6121 gcggacggta gcgcaggcgg cctcggcgcc gtcgtggcgt cgcgatggtt ggcgccagac
     6181 ccgcctgatc ggcgtcccga ccagggccgc cggggtggtg agcctcggct tggtccagcc
     6241 gtagtgagac gggatgtctt cccggttgtc gggtttgggt gggcggtcac atgttcgctg
     6301 gctgtgggcg tgtcgggggt cgtcgaagcg gccgcgaaca gggctggacg atgcacgtct
     6361 tgcacctcct gtcaaccccc aatgttgagc cgcgatgccc ctgacctgca ccgttggacc
     6421 gcagtcgtta ggggggcgtg ttagcatgga gtgacgaaag gggcctaaat cagatgatcc
//
