LOCUS       sequence-327027-       11572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of proneural Glioblastoma 
            cell-state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Schmitt MJ, Company C, Dramaretska Y, Barozzi I, Gohrig A, Kertalli 
            S, Grossmann M, Naumann H, Sanchez-Bailon MP, Hulsman D, Glass R, 
            Squatrito M, Serresi M, Gargiulo G
  TITLE     Phenotypic Mapping of Pathologic Cross-Talk between Glioblastoma and
            Innate Immune Cells by Synthetic Genetic Tracing.
  JOURNAL   Cancer Discov. 2021 Mar;11(3):754-777. doi: 
            10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23.
  PUBMED    33361384
REFERENCE   2  (bases 1 to 11572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-20-0219"; journalName: "Cancer Discov"; date: 
            "2021-03"; volume: "11"; issue: "3"; pages: "754-777"
COMMENT     Sequence Label: FH1-PNGT#1-mCherry_PGK-H2B-CFP
FEATURES             Location/Qualifiers
     source          1..11572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3430..3448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3466..3484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3534..4241
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3676..3694)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3944..3963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4400..4988
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4453..4473)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4871..4882)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5112..5622
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5541..5559
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5634..6011
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      6024..6033
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6030..6032,6033..6035,6036..6749)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6075..6096)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6336..6355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6683..6704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             7140..7320
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7346..7363)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7352..7576
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7622..8050
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7709..7728)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7919..7940
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8059..8079)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8064..8393
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8244..8379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8306..8325
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8441..8488
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8507..8881
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9011..9132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9048..9067)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9102..9121
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9181..9197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9181..9197)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9194..9216)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9205..9221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9229..9235,9236..9253,9254..9259))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9274..9295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9412..9429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9583..10171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9663..9682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10342..11133,11134..11202))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10965..10984
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11203..11307)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11382..11401)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaaagagct cctggccaag gtctttgtgt tcagaccaga
     2401 agaggaagga gggctccctc cccctggggc tgtggaggct gaggctcctg gggggttgtc
     2461 cacatctgga ccgtgggagc tgttgggggg aatgggggca ggtggagaag aggataagca
     2521 gctgattggg cccagactac tctgggctgg ctccatctta catgactgcc acaaacagct
     2581 gcaggagtgt gacagatcac aacactagca ttgtacctca aaatatgctt gtaccctaag
     2641 gcacaagaac tggtttgact tacaaccgca gcccccgtcc gggcaccccg aggcccgcgg
     2701 gagccaccct cgaaccccgg ccgcgcacgg gcggggcggc gcgcacctgc cgggagcccg
     2761 tgtttgtaaa caaaccgcgc gcctaattag cctggcggga gcgcgcgcgc ggggcggggg
     2821 gcggggcgtc ggtgcgcgcg ggcaggtcgg ccccgcccgg ggaggagccg cgctctgccg
     2881 cgccctccgt gtcaccatct cccccacccg acttggcggg gcgcgggctt gctggagcct
     2941 gcgggaccca gagcccgctc cggagccagc cctgggagtg gccagcttga acccgagggc
     3001 cccgcagacc gttactccgg cccccgcccg gggcggggcg cgcgggggcg cggcgcagcc
     3061 caacccgcac agccgcgtcc ccaaacacca ccgaggaggg aaaacagacg gagaggggtg
     3121 gggctgcggg cggggccggc gcctaattgg gccgcgggcg cctcgaggtg ggcggggcat
     3181 aagggggcgg ggccgcggag accccgggcg ggagcaggga gaggaaagaa gagactgagt
     3241 acgcggagac cgagattcgg aaatatttct gccttaattg ttcttccatt gtctttctcc
     3301 tgtgggtccc ctctcacctt tctgtatggt cctggatcac cccccgaggc tttgtctccc
     3361 ccatccacgg gcttattctc tcggcacccc cttcctctcc cgtcatcggt tgattgtaca
     3421 ctagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     3481 gagaattcat gtatggcagg aagaagcgga gacagcgacg aagaaagctt ggggtgagca
     3541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     3601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     3661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     3721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     3781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     3841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     3901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     3961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     4021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     4081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     4141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     4201 gccgccactc caccggcggc atggacgagc tatacaagta aaccggcggc atggacgagc
     4261 tatacaagta aaccggtatc tagataactg atcataatca gccataccac atttgtagag
     4321 gttttacttg ctttaaaaaa cctcccacac gtacaagtaa agcggccgcg atctacaatt
     4381 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4441 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4501 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4561 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4621 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4681 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4741 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4801 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4861 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4921 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4981 ctccccgcat cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     5041 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     5101 acctcaggta cggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga
     5161 cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca
     5221 ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg
     5281 gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg
     5341 acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga
     5401 gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc
     5461 gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg
     5521 gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca
     5581 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatct accatgccag
     5641 agccagcgaa gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc
     5701 agaagaaagg cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtgt
     5761 acaaggttct gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca
     5821 tgaactcgtt tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc
     5881 attacaacaa gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc
     5941 tgcctgggga gttggccaag cacgccgtgt ccgagggtac taaggccatc accaagtaca
     6001 ccagcgctaa ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
     6061 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacagg ttcagcgtgt
     6121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     6181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt
     6241 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     6301 aaggctacgt ccaggagcgt accatcttct tcaaggacga cggcaactac aagacccgcg
     6361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     6421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc agccacaacg
     6481 tctatatcac cgccgacaag cagaagaacg gcatcaaggc ccacttcaag atccgccaca
     6541 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     6601 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     6661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     6721 ctctcggcat ggacgagctg tacaagtagg tacctttaag accaatgact tacaaggcag
     6781 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6841 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     6901 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     6961 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     7021 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     7081 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     7141 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7201 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7261 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7321 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7381 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7441 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7501 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7561 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     7621 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7681 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7741 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7801 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7861 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7921 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7981 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8041 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8101 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     8161 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8221 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8281 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     8341 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     8401 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     8461 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     8521 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     8581 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     8641 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     8701 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     8761 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     8821 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     8881 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     8941 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     9001 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9061 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9121 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     9181 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9241 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9361 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9421 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9481 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10021 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10621 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    11341 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
    11401 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
    11461 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
    11521 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
