LOCUS       sequence-327028-        9930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of proneural Glioblastoma 
            cell-state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Schmitt MJ, Company C, Dramaretska Y, Barozzi I, Gohrig A, Kertalli 
            S, Grossmann M, Naumann H, Sanchez-Bailon MP, Hulsman D, Glass R, 
            Squatrito M, Serresi M, Gargiulo G
  TITLE     Phenotypic Mapping of Pathologic Cross-Talk between Glioblastoma and
            Innate Immune Cells by Synthetic Genetic Tracing.
  JOURNAL   Cancer Discov. 2021 Mar;11(3):754-777. doi: 
            10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23.
  PUBMED    33361384
REFERENCE   2  (bases 1 to 9930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-20-0219"; journalName: "Cancer Discov"; date: 
            "2021-03"; volume: "11"; issue: "3"; pages: "754-777"
COMMENT     Sequence Label: FH1-PNGT#1-mCherry
FEATURES             Location/Qualifiers
     source          1..9930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3430..3448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3466..3484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3534..4241
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3676..3694)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3944..3963
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4400..4988
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4453..4473)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4871..4882)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5498..5678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5704..5721)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5710..5934
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5980..6408
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6067..6086)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6277..6298
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6417..6437)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6422..6751
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6602..6737
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6664..6683
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6799..6846
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6865..7239
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7369..7490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7406..7425)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7460..7479
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7539..7555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7539..7555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7552..7574)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7563..7579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7587..7593,7594..7611,7612..7617))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7632..7653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7770..7787)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7941..8529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8021..8040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8700..9491,9492..9560))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9323..9342
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9561..9665)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9740..9759)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaaagagct cctggccaag gtctttgtgt tcagaccaga
     2401 agaggaagga gggctccctc cccctggggc tgtggaggct gaggctcctg gggggttgtc
     2461 cacatctgga ccgtgggagc tgttgggggg aatgggggca ggtggagaag aggataagca
     2521 gctgattggg cccagactac tctgggctgg ctccatctta catgactgcc acaaacagct
     2581 gcaggagtgt gacagatcac aacactagca ttgtacctca aaatatgctt gtaccctaag
     2641 gcacaagaac tggtttgact tacaaccgca gcccccgtcc gggcaccccg aggcccgcgg
     2701 gagccaccct cgaaccccgg ccgcgcacgg gcggggcggc gcgcacctgc cgggagcccg
     2761 tgtttgtaaa caaaccgcgc gcctaattag cctggcggga gcgcgcgcgc ggggcggggg
     2821 gcggggcgtc ggtgcgcgcg ggcaggtcgg ccccgcccgg ggaggagccg cgctctgccg
     2881 cgccctccgt gtcaccatct cccccacccg acttggcggg gcgcgggctt gctggagcct
     2941 gcgggaccca gagcccgctc cggagccagc cctgggagtg gccagcttga acccgagggc
     3001 cccgcagacc gttactccgg cccccgcccg gggcggggcg cgcgggggcg cggcgcagcc
     3061 caacccgcac agccgcgtcc ccaaacacca ccgaggaggg aaaacagacg gagaggggtg
     3121 gggctgcggg cggggccggc gcctaattgg gccgcgggcg cctcgaggtg ggcggggcat
     3181 aagggggcgg ggccgcggag accccgggcg ggagcaggga gaggaaagaa gagactgagt
     3241 acgcggagac cgagattcgg aaatatttct gccttaattg ttcttccatt gtctttctcc
     3301 tgtgggtccc ctctcacctt tctgtatggt cctggatcac cccccgaggc tttgtctccc
     3361 ccatccacgg gcttattctc tcggcacccc cttcctctcc cgtcatcggt tgattgtaca
     3421 ctagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     3481 gagaattcat gtatggcagg aagaagcgga gacagcgacg aagaaagctt ggggtgagca
     3541 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     3601 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     3661 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     3721 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     3781 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     3841 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     3901 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     3961 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     4021 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     4081 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     4141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     4201 gccgccactc caccggcggc atggacgagc tatacaagta aaccggcggc atggacgagc
     4261 tatacaagta aaccggtatc tagataactg atcataatca gccataccac atttgtagag
     4321 gttttacttg ctttaaaaaa cctcccacac gtacaagtaa agcggccgcg atctacaatt
     4381 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4441 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4501 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4561 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4621 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4681 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4741 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4801 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4861 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4921 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4981 ctccccgcat cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     5041 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     5101 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5161 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     5221 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     5281 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     5341 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     5401 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     5461 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     5521 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5581 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5641 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     5701 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     5761 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     5821 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     5881 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     5941 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     6001 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6061 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6121 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6181 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6241 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6301 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6361 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     6421 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6481 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     6541 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     6601 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6661 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     6721 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     6781 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     6841 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     6901 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     6961 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     7021 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     7081 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     7141 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     7201 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     7261 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     7321 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     7381 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7441 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     7501 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7561 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7621 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7681 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7741 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7801 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7861 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7921 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7981 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8041 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8101 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8161 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8221 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8281 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8341 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8401 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8461 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8521 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8581 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8641 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8701 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8761 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8821 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8881 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8941 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9001 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9061 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9121 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9181 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9241 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9301 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9361 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9421 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9481 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9541 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9601 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9661 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     9721 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     9781 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     9841 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     9901 ttcgcgatgt acgggccaga tatacgcgtt
//
