LOCUS       sequence-327033-       11427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma
            cell-state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Schmitt MJ, Company C, Dramaretska Y, Barozzi I, Gohrig A, Kertalli 
            S, Grossmann M, Naumann H, Sanchez-Bailon MP, Hulsman D, Glass R, 
            Squatrito M, Serresi M, Gargiulo G
  TITLE     Phenotypic Mapping of Pathologic Cross-Talk between Glioblastoma and
            Innate Immune Cells by Synthetic Genetic Tracing.
  JOURNAL   Cancer Discov. 2021 Mar;11(3):754-777. doi: 
            10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23.
  PUBMED    33361384
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-20-0219"; journalName: "Cancer Discov"; date: 
            "2021-03"; volume: "11"; issue: "3"; pages: "754-777"
COMMENT     Sequence Label: FH1-MGT#1-mVenus_PGK-H2B-CFP
FEATURES             Location/Qualifiers
     source          1..11427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3210..3228
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3246..3264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(3329..3331,3332..4042)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    4255..4843
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4308..4328)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4726..4737)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4967..5477
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5396..5414
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5489..5866
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      5879..5888
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5885..5887,5888..5890,5891..6604)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     LTR             6995..7175
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7201..7218)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7207..7431
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7477..7905
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7564..7583)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7774..7795
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7914..7934)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7919..8248
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8099..8234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8161..8180
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8296..8343
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8362..8736
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8866..8987
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8903..8922)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8957..8976
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9036..9052)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9036..9052)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9049..9071)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9060..9076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9084..9090,9091..9108,9109..9114))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9129..9150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9267..9284)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9438..10026)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9518..9537)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10197..10988,10989..11057))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10820..10839
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11058..11162)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11237..11256)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaatgcata tttattttta ggaccagaaa gttaaagtga
     2401 attggatttg atccattttc tgaaaggctg gcaagaattc ttgacattgc acaggaattt
     2461 ccatgtcagc atgttctcac atgtatgatc taatttagag attattttgg ggggcggggg
     2521 ttgaggaaat ggcatgactc agagtttaaa agccccaaat cttagctgtg cctgtgtagc
     2581 tttaccacat aacccattga taacttagtt gtgcaaccat caccaccatc tgttttcaga
     2641 actcttttca ttttgcgaaa ctgaaacccg ttaagcactg atttcccact ctccctcctc
     2701 ccagcccata gcaaaccacc atcccaccag cactttcatt tcgcaaatgg caaaactgaa
     2761 gccgatattg tggttgtgac ttatcccaaa gtaatataca cataaacctc tatggatgag
     2821 gaaaaagaca gagggaaact aaaaattcaa aagaacaaat ttgactcaca gatttgctga
     2881 ctcatagttg tgacacttcc tggctcagga agttgaattt cattaagcct ttgtggtttg
     2941 gggctctgct gtgctttgac agctctgatc tcctcccttc cggctgggct gtctggggcg
     3001 ctctaaaatg agtgttgatt taatgcactg ccttcgcacc cgtgctggtg cgtcccgggg
     3061 acaggggtgg ctgtgcggtg ccgcggcggc cggcggggct ccttccccag caggggtggg
     3121 gacgctgagt cacggatctg tcaccgcttt gcacctctcc gagccctcgg gggccaaagc
     3181 aaaagcgaaa gcgatgtaca ctagtttact ccctatcagt gatagagaac gtatatccag
     3241 tttactccct atcagtgata gagaacgtat ggagaccgac accctgctgc tgtgggtgct
     3301 gctgctgtgg gtgcccggca gcaccggcgt gagcaagggc gaggagctgt tcaccggggt
     3361 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3421 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctcatct gcaccaccgg
     3481 caagctgccc gtgccctggc ccaccctcgt gaccaccctc ggctacggcc tgcagtgctt
     3541 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3601 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3661 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3721 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3781 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     3841 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3901 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
     3961 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4021 cggcatggac gagctctaca agtccggact cagatctcga gctgctgctg cagtcctggt
     4081 gctgttggtg attgtgatca tctcacttat tgtcctggtt gtcatttggt gaatctagat
     4141 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     4201 cacacgtaca agtaaagcgg ccgcgatcta caattcgata tcaagcttat cgataatcaa
     4261 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4321 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4381 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4441 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4501 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4561 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4621 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     4681 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4741 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4801 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgaga cctagaaaaa
     4861 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     4921 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtacgggg ttggggttgc
     4981 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     5041 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     5101 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     5161 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     5221 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     5281 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     5341 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     5401 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     5461 ccgacctctc tccccagggg gatctaccat gccagagcca gcgaagtctg ctcccgcccc
     5521 gaaaaagggc tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa
     5581 gcgcagccgc aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc
     5641 tgacaccggc atttcgtcca aggccatggg catcatgaac tcgtttgtga acgacatttt
     5701 cgagcgcatc gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac
     5761 ctccagggag atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc
     5821 cgtgtccgag ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc
     5881 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     5941 ggacggcgac gtaaacggcc acaggttcag cgtgtccggc gagggcgagg gcgatgccac
     6001 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     6061 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat
     6121 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgtaccat
     6181 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     6241 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     6301 gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa
     6361 gaacggcatc aaggcccact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     6421 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     6481 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     6541 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     6601 gtaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6661 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     6721 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     6781 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     6841 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     6901 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     6961 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     7021 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7081 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7141 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     7201 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     7261 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     7321 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     7381 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     7441 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     7501 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7561 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7621 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7681 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7741 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7801 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7861 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     7921 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7981 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8041 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8101 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8161 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     8221 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     8281 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     8341 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     8401 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     8461 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     8521 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     8581 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     8641 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     8701 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     8761 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     8821 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     8881 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8941 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     9001 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9061 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9121 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9181 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9241 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9301 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9361 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9421 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9481 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9541 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9601 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9661 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9721 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9781 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9841 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9901 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9961 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10021 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10081 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10141 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10201 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10261 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10321 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10381 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10441 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10501 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10561 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10621 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10681 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10741 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10801 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10861 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10921 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10981 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    11041 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11101 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11161 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
    11221 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
    11281 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
    11341 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
    11401 gcgatgtacg ggccagatat acgcgtt
//
