LOCUS       sequence-327034-        9913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma
            cell-state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Schmitt MJ, Company C, Dramaretska Y, Barozzi I, Gohrig A, Kertalli 
            S, Grossmann M, Naumann H, Sanchez-Bailon MP, Hulsman D, Glass R, 
            Squatrito M, Serresi M, Gargiulo G
  TITLE     Phenotypic Mapping of Pathologic Cross-Talk between Glioblastoma and
            Innate Immune Cells by Synthetic Genetic Tracing.
  JOURNAL   Cancer Discov. 2021 Mar;11(3):754-777. doi: 
            10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23.
  PUBMED    33361384
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-20-0219"; journalName: "Cancer Discov"; date: 
            "2021-03"; volume: "11"; issue: "3"; pages: "754-777"
COMMENT     Sequence Label: FH1-MGT#2-mCherry
FEATURES             Location/Qualifiers
     source          1..9913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3413..3431
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3449..3467
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3517..4224
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3659..3677)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3927..3946
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4383..4971
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4436..4456)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4854..4865)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5481..5661
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5687..5704)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5693..5917
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5963..6391
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6050..6069)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6260..6281
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6400..6420)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6405..6734
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6585..6720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6647..6666
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6782..6829
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6848..7222
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7352..7473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7389..7408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7443..7462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7522..7538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7522..7538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7535..7557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7546..7562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7570..7576,7577..7594,7595..7600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7615..7636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7753..7770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7924..8512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8004..8023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8683..9474,9475..9543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9306..9325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9544..9648)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9723..9742)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaatgcatc tagaacagca gggccacctc cttctctccc
     2401 ccgcgggcat gggcccccac ccccactgcc ggcagagtgc tgaggactcg tgcaccatga
     2461 gaacttctga ccatgagaac tttgacttcc ggatttgggg gatctgccca ggtgaacaca
     2521 atgcaagggg ctgcatgacc taccaggaca gaactttccc caattacagg gtgactcaca
     2581 gccgcattgg tgactcactt caatgtgtca tttccggctg ctgtgtgtga gcagtggaca
     2641 cgtgaggggg gggtgggtga gagagacagg ccacattgtg caacagatct ctagagcttt
     2701 ttcatcttgc aaaactgaaa ctgtataccc atggaacaac agctccctgc tcccctcccc
     2761 ctcagctcct gggtagtgac atttcttgat tctcagtaaa ctatcacaag aacaaaaaac
     2821 caaacaccgc atattctcac tcataggtgg gaattgaaca atgagatcac atggacacag
     2881 gaaggggaat atcacactct ggggactgtg gtggggtggg gggagtgggg agggatagca
     2941 ctgggatgtc ccaagagaag gggaagaggg ggaggtgtta gagaacttgt gtgttcaacc
     3001 gaaacatgat gaaaacaggg aaagccccca agatacctgt cattcccgat gatgtcagat
     3061 tcagcaaatt caatgataac aaaacattat gaaaaaatta gtaattaaaa taatacagca
     3121 atgtgtatga acaaaataat caatgaaagt gaaacctaat agtaattcca caaacttatt
     3181 acaaagctat taatttaaag agtagtggca attgaaaacc acaaccaaca ccagtgctta
     3241 cagcagcaat acttttactc agacttcctg tttctggaac ttgccttctt ttttgctgtg
     3301 tttatacttc ccttgtctgt ggttagataa gtataaagcc ctagatctaa gcttctctgt
     3361 cttcctccct ccctcccttc ctcttactct cattcattgt acactagttt actccctatc
     3421 agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaatt catgtatggc
     3481 aggaagaagc ggagacagcg acgaagaaag cttggggtga gcaagggcga ggaggataac
     3541 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     3601 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     3661 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     3721 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     3781 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     3841 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     3901 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     3961 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     4021 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     4081 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     4141 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     4201 ggcatggacg agctatacaa gtaaaccggc ggcatggacg agctatacaa gtaaaccggt
     4261 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     4321 aaacctccca cacgtacaag taaagcggcc gcgatctaca attcgatatc aagcttatcg
     4381 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4441 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4501 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4561 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4621 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4681 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4741 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     4801 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     4861 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4921 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgagacc
     4981 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     5041 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     5101 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5161 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     5221 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     5281 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     5341 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     5401 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     5461 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5521 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5581 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5641 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     5701 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5761 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5821 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5881 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     5941 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6001 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6061 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6121 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6181 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6241 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6301 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6361 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     6421 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6481 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6541 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6601 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6661 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6721 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     6781 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     6841 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     6901 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     6961 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     7021 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     7081 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     7141 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     7201 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     7261 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7321 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     7381 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7441 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     7501 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7561 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7621 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7681 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7801 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8341 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8521 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8581 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8641 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8701 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8761 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8821 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8881 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8941 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9001 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9061 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9121 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9181 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9241 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9301 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9361 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9421 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9481 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9541 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9601 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9661 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     9721 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     9781 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     9841 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     9901 agatatacgc gtt
//
