LOCUS       sequence-327040-        9785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma
            cell-state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Schmitt MJ, Company C, Dramaretska Y, Barozzi I, Gohrig A, Kertalli 
            S, Grossmann M, Naumann H, Sanchez-Bailon MP, Hulsman D, Glass R, 
            Squatrito M, Serresi M, Gargiulo G
  TITLE     Phenotypic Mapping of Pathologic Cross-Talk between Glioblastoma and
            Innate Immune Cells by Synthetic Genetic Tracing.
  JOURNAL   Cancer Discov. 2021 Mar;11(3):754-777. doi: 
            10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23.
  PUBMED    33361384
REFERENCE   2  (bases 1 to 9785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-20-0219"; journalName: "Cancer Discov"; date: 
            "2021-03"; volume: "11"; issue: "3"; pages: "754-777"
COMMENT     Sequence Label: FH1-MGT#1-mVenus
FEATURES             Location/Qualifiers
     source          1..9785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3210..3228
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3246..3264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(3329..3331,3332..4042)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3371..3392)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3632..3651)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3979..4000
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4255..4843
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4308..4328)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4726..4737)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5353..5533
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5559..5576)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5565..5789
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5835..6263
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5922..5941)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6132..6153
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6272..6292)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6277..6606
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6457..6592
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6519..6538
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6654..6701
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6720..7094
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7224..7345
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7261..7280)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7315..7334
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7394..7410)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7394..7410)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7407..7429)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7418..7434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7442..7448,7449..7466,7467..7472))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7487..7508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7625..7642)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7796..8384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7876..7895)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8555..9346,9347..9415))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9178..9197
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9416..9520)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9595..9614)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaatgcata tttattttta ggaccagaaa gttaaagtga
     2401 attggatttg atccattttc tgaaaggctg gcaagaattc ttgacattgc acaggaattt
     2461 ccatgtcagc atgttctcac atgtatgatc taatttagag attattttgg ggggcggggg
     2521 ttgaggaaat ggcatgactc agagtttaaa agccccaaat cttagctgtg cctgtgtagc
     2581 tttaccacat aacccattga taacttagtt gtgcaaccat caccaccatc tgttttcaga
     2641 actcttttca ttttgcgaaa ctgaaacccg ttaagcactg atttcccact ctccctcctc
     2701 ccagcccata gcaaaccacc atcccaccag cactttcatt tcgcaaatgg caaaactgaa
     2761 gccgatattg tggttgtgac ttatcccaaa gtaatataca cataaacctc tatggatgag
     2821 gaaaaagaca gagggaaact aaaaattcaa aagaacaaat ttgactcaca gatttgctga
     2881 ctcatagttg tgacacttcc tggctcagga agttgaattt cattaagcct ttgtggtttg
     2941 gggctctgct gtgctttgac agctctgatc tcctcccttc cggctgggct gtctggggcg
     3001 ctctaaaatg agtgttgatt taatgcactg ccttcgcacc cgtgctggtg cgtcccgggg
     3061 acaggggtgg ctgtgcggtg ccgcggcggc cggcggggct ccttccccag caggggtggg
     3121 gacgctgagt cacggatctg tcaccgcttt gcacctctcc gagccctcgg gggccaaagc
     3181 aaaagcgaaa gcgatgtaca ctagtttact ccctatcagt gatagagaac gtatatccag
     3241 tttactccct atcagtgata gagaacgtat ggagaccgac accctgctgc tgtgggtgct
     3301 gctgctgtgg gtgcccggca gcaccggcgt gagcaagggc gaggagctgt tcaccggggt
     3361 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3421 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctcatct gcaccaccgg
     3481 caagctgccc gtgccctggc ccaccctcgt gaccaccctc ggctacggcc tgcagtgctt
     3541 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3601 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3661 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3721 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3781 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     3841 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3901 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
     3961 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4021 cggcatggac gagctctaca agtccggact cagatctcga gctgctgctg cagtcctggt
     4081 gctgttggtg attgtgatca tctcacttat tgtcctggtt gtcatttggt gaatctagat
     4141 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     4201 cacacgtaca agtaaagcgg ccgcgatcta caattcgata tcaagcttat cgataatcaa
     4261 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4321 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4381 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4441 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4501 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4561 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4621 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     4681 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4741 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4801 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgaga cctagaaaaa
     4861 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     4921 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     4981 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     5041 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     5101 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     5161 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     5221 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     5281 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     5341 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5401 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5461 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5521 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     5581 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     5641 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     5701 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     5761 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     5821 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5881 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5941 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6001 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6061 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6121 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     6181 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     6241 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     6301 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6361 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6421 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6481 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6541 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     6601 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     6661 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     6721 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     6781 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     6841 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     6901 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     6961 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     7021 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     7081 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     7141 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     7201 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     7261 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7321 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     7381 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7441 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7501 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7561 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7621 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7681 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7741 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7801 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7861 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7921 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7981 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8041 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8101 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8161 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8221 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8281 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8341 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8401 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8461 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8521 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8581 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8641 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8701 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8761 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8821 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8881 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8941 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9001 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9061 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9121 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9181 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9241 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9301 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9361 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9421 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9481 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9541 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     9601 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     9661 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     9721 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     9781 gcgtt
//
