LOCUS       sequence-327043-       11012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11012)
  TITLE     Immunoprecipitation of the endoplasmic reticulum (ER-IP)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: ER-P-TAG (pGS594)
FEATURES             Location/Qualifiers
     source          1..11012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3352..4538
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3583..4529
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4486..4506
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4619..4628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4784..5530
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             5531..5620
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     misc_feature    5633..6185
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5777..5794)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6004..6023
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6194..6589
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             6599..6655
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6665..7210
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             7220..7240
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7247..7835
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7300..7320)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7718..7729)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8221..8454
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8526..8647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8563..8582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8617..8636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8749..8768
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(9033..9051)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9119..9223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9224..9292,9293..10084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9442..9461)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10255..10843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10744..10763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgata ccgtcgagtt tactccctat
     2281 tctagacaaa tgcttgtcct tccttcctgt aacacagcca ttaaaccagg agcatcgccc
     2341 ttccccggcc ctcaggtaag aggaccaaat accgtagccg tttccaattt cagtccttta
     2401 gcgccacctg gtgctaacta ctctatcacg cttttatcca ataactacct ttgtaaattt
     2461 cctttcaaaa gttctggccg ggcgcggtgg ctcacgcttg taatcccagc actttgtgag
     2521 gggtcaggag ttcgagacat taatagggag gtggtccctg cagttacgcc aatgataacc
     2581 cccgccagaa aaatcttagt agccttccct ttttgttttc cgtgccccaa ctcggcggat
     2641 tgactcggcc ccttccggaa acacccgaat caacttctag tcaaattatt gttcacgccg
     2701 caatgaccca cccctggccc gcgtctgtgg aactgacccc tggtgtacag gagagttcgc
     2761 tgctgaaagt ggtcccaaag gggtactagt ttttaagctc ccaactcccc ctcccccagc
     2821 gtctggagga ttccacaccc tcgcaccgca ggggcgagga agtgggcgga gtccggtttt
     2881 ggcgccagcc gctgaggctg ccaagcagaa aagccaccgc tgaggagact ccggtcactg
     2941 tcctcgcccc gcctccccct tccctcccct tggggaccac cgggcgccac gccgcgaacg
     3001 gtaagtgccg cggtcgtcgg cgcctccgcc ctccccctag ggccccaatt cccagcgggc
     3061 gcggcgcgcg gcccctcccc ccgccgggcg cgcgcccgct gccccgccct tcgtggccgc
     3121 ccggcgtggg cggtgccacc cctccccccg gcggccccgc gcgcagctcc cggctccctc
     3181 ccccttcgga tgtggcttga gctgtagcgc ggagtttaat taaaatcgcg taccgtcgac
     3241 ctcgacggat cgggagatct cgaccgagct ttgcaaagat ggataaagtt ttaaacagag
     3301 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
     3361 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     3421 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     3481 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     3541 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     3601 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
     3661 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
     3721 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
     3781 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
     3841 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
     3901 cttgtaaatg cgggccaaga tcgatctgca cactggtatt tcggtttttg gggccgcggg
     3961 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     4021 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     4081 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     4141 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     4201 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     4261 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     4321 ctcgatcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     4381 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     4441 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     4501 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattagcttg accaagcttg
     4561 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattatc acaaggccgc
     4621 caccatggac cctgtggtcg tgctggggct gtgcctgagc tgtctgctgc tgctgagcct
     4681 gtggaagcag tcctacggag gcgggggaag tggctcaggg ggtcgggggt caggttcagg
     4741 cagtggctct ggcagcggta gcggcagtgg ctctgggtca ggaggaaagt cttacccaac
     4801 tgtgagtgct gattaccagg acgccgttga gaaggcgaag aagaagctca gaggcttcat
     4861 cgctgagaag agatgcgctc ctctaatgct ccgtttggca ttccactctg ctggaacctt
     4921 tgacaagggc acgaagaccg gtggaccctt cggaaccatc aagcaccctg ccgaactggc
     4981 tcacagcgct aacaacggtc ttgacatcgc tgttaggctt ttggagccac tcaaggcgga
     5041 gttccctatt ttgagctacg ccgatttcta ccagttggct ggcgttgttg ccgttgaggt
     5101 cacgggtgga cctaaggttc cattccaccc tggaagagag gacaagcctg agccaccacc
     5161 agagggtcgc ttgcccgatc ccactaaggg ttctgaccat ttgagagatg tgtttggcaa
     5221 agctatgggg cttactgacc aagatatcgt tgctctatct gggggtcaca ctattggagc
     5281 tgcacacaag gagcgttctg gatttgaggg tccctggacc tctaatcctc ttattttcga
     5341 caactcatac ttcacggagt tgttgagtgg tgagaaggaa ggtctccttc agctaccttc
     5401 tgacaaggct cttttgtctg accctgtatt ccgccctctc gttgacaaat atgcagcgga
     5461 cgaagatgcc ttctttgctg attacgctga ggctcaccaa aagctttccg agcttgggtt
     5521 tgctgatgcc tacccatacg atgttcctga ctatgcgggc tatccgtatg acgtcccgga
     5581 ctatgcagga tcctatccat atgacgttcc agattacgct taataaaaac taacgttact
     5641 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     5701 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     5761 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     5821 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     5881 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     5941 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     6001 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     6061 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     6121 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     6181 taatatggcc acaatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     6241 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     6301 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     6361 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     6421 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     6481 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     6541 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcg gatccggcgc
     6601 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgggatc
     6661 cggcatggac aaagactgcg aaatgaagcg caccaccctg gatagccctc tgggcaagct
     6721 ggaactgtct gggtgcgaac agggcctgca ccgtatcatc ttcctgggca aaggaacatc
     6781 tgccgccgac gccgtggaag tgcctgcccc agccgccgtg ctgggcggac cagagccact
     6841 gatgcaggcc accgcctggc tcaacgccta ctttcaccag cctgaggcca tcgaggagtt
     6901 ccctgtgcca gccctgcacc acccagtgtt ccagcaggag agctttaccc gccaggtgct
     6961 gtggaaactg ctgaaagtgg tgaagttcgg agaggtcatc agctacagcc acctggccgc
     7021 cctggccggc aatcccgccg ccaccgccgc cgtgaaaacc gccctgagcg gaaatcccgt
     7081 gcccattctg atcccctgcc accgggtggt gcagggcgac ctggacgtgg ggggctacga
     7141 gggcgggctc gccgtgaaag agtggctgct ggcccacgag ggccacagac tgggcaagcc
     7201 tgggctgggt ggcgggagcc caaagaagaa gcggaaggtg tagcggaatc aacctctgga
     7261 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7321 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7381 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7441 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7501 caccacctgt cagctccttt ccggaacttt cgctttcccc ctccctattg ccacggcgga
     7561 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7621 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct atgttgccac
     7681 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7741 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7801 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgaggt
     7861 acgccaacag agcgagattc cgtctcaaag aaaaaaaaag taatgaaatg aataaaatga
     7921 gtcctagagc cagtaaatgt cgtaaatgtc tcagctagtc aggtagtaaa aggtctcaac
     7981 taggcagtgg cagagcagga ttcaaattca gggctgttgt gatgcctccg cagactctga
     8041 gcgccacctg gtggtaattt gtctgtgcct cttctgacgt tgaagaacag caactaacac
     8101 actaacacgg catttactat gggccagcca ttgttctaat gttaactgtg gtacctttaa
     8161 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8221 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     8281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8461 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8521 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8641 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     8701 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8761 ggccgaggcc ggatcccttg agtggctttc atcctggagc agactttgca gtctgtggac
     8821 tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg
     8881 ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga
     8941 ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata
     9001 aggccccctt gttaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     9061 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     9121 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9181 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9241 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9301 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9361 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9421 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     9481 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9541 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9601 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9661 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9721 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
     9781 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9841 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9901 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     9961 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10021 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10081 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10141 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10201 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    10261 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10321 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10381 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
    10441 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10501 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    10561 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    10621 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    10681 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    10741 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    10801 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    10861 ctttttacgg ttcctggcct tttgctggcc ttgaagctgt ccctgatggt cgtcatctac
    10921 ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat
    10981 cataatgggg aaggccatcc agcctcgcgt cg
//
