LOCUS       sequence-327045-        6352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish kcnma1a gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pDONR221-kcnma1a
FEATURES             Location/Qualifiers
     source          1..6352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(68..95)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(187..273)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     322..344
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     336..353
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     337..353
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    369..468
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4277..4376)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4393..4412)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4394..4412)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4417..4433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4417..4433)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4535..4554)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4546..5355
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4613..4632)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5502..6090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5991..6010
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6244..6261
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
       61 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
      121 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
      181 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
      241 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
      301 taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc
      361 tcgggcccca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
      421 atgagcaatg cttttttata atgccaactt tgtacaaaaa agcaggctcc gcggccgccc
      481 ccttcaccat gctagctgtg gcagatggca acggatccta tggcagcagc agctctagaa
      541 tgagtaataa tatcaatttt aataaaaatc cggactcttc tgtctctata tcgaagatgg
      601 acgtgatcat cccgttcaca ccagacgtac cctgtgacaa caatggtcag cgcatgtggt
      661 gggctttcct cgcctcatct atggtgacgt tcttcggggg tctcttcatc atcctcctgt
      721 ggaggaccct caagtacctg tggactgtct gctgccattg caacatcaaa aacaaggagg
      781 cacagaaggt gaataaccct attactattc aggctgatgg cacaacaaag actgggaatg
      841 aaaaagaaga agccccagcc agtgaagtgg gctggatgac ctcagtcaaa gactgggccg
      901 gggtcatgat ctcagcacag accctcaccg gcagagtttt ggtggtgttg gtctttgcac
      961 tcagcatcgg agctctggga atatacttca tagactcttc ggatcctata gaatcctgtc
     1021 agaatttcta caaagacttc acgttacaaa ttgacatggc attcaacgtg ttcttcttac
     1081 tctactttgg tctgcgcttc atagcagcca atgataagct gtggttctgg ctggaggtca
     1141 actcagtggt ggacttcttc accgtgcctc ccgtgtttgt gtcagtgtac ctgaacagga
     1201 gttggcttgg tctgaggttt ctaagagcct tgagattgat acagttctca gaaattttac
     1261 agtttttgaa tatcttaaaa acaagtaatt ccatcaagct ggtgaacctg tgttccatct
     1321 tcataagcac atggctcact gctgcaggat tcattcatct ggtggaaaac tcaggggatc
     1381 catgggaaaa cttccaaaac tctcaaccgc tctcatattg ggaatgtgtg tacttattaa
     1441 tggtcactat gtccacggtg ggatatgggg acgtctatgc aagaaccact cttggacgcc
     1501 ttttcatggt cttcttcatt ctcggtggtc tggccatgtt tgccagctac gtccctgaaa
     1561 tcatagagtt aataggaaac cgcaagaaat atggtggttc ctatagtgcg gtaaatggca
     1621 gaaagcatat tgtggtctgt ggtcatatca cgctcgaaag tgtttccaac ttccttaaag
     1681 acttcctaca caaggacagg gatgatgtca atgtagaaat tgtttttctt catgatattt
     1741 cccctaatct tgagctggaa gccttgttca aacgtcactt cactcaggtg gaattctatc
     1801 aaggatcggt cctcaaccca catgatctag caagagtgaa gattgagtcg gcggacgctt
     1861 gcttgattct tgcaaacaaa tactgtgcag accccgatgc tgaggatgcc tcaaatatca
     1921 tgagagtgat atctatcaag aactaccatc caaagatcag aataataaca caaatgttgc
     1981 agtaccacaa caaggcccat ctgctaaaca ttcccagctg gaactggaag gagggagatg
     2041 atgcgatttg cctggccgag ttgaaggcag gcttcatagc ccagagctgc ctggcacagg
     2101 gcctgtccac catgctcgcc aacctcttct ccatgaggtc atacatcaag attgaagaag
     2161 acacatggca gaagtattat ctggaaggag tagccaatga aatgtacact gagtacctgt
     2221 ccagtgcctt tgtcggcctt tcatttccta cagtttgtga gctgtgctac gtgaagctca
     2281 agttgctgtt gatcgctatt gagtacaagt cagagcaaag ggaaagcaga agcagaaagc
     2341 gtattctgat caacccggga aatcacgtta aaatgcagga ggggacactg ggattcttta
     2401 ttgccagtga tgccaaagaa gtgaaaagag catttttcta ctgcaaagca tgtcatgatg
     2461 acatcacaga tcccaaacgg attaaaaagt gtggctgcaa gagaatgaca tattccaaga
     2521 tgtccgtcta caagcgaatg aaactggcat gttgttttga ttgcgggcgt tctgagcgcg
     2581 actgctcgtg catgtcaggc agtgtacata gtaacatgga cagccttcag agggccttcc
     2641 cactttcttc tgtctctgtt catgattgct caaccagttt acgtgccttc gaagatgagc
     2701 atccgtcaac actgtcaccc aaaaagaagc agcgcaacgg aggcatgcga aattcaccca
     2761 actgctcgcc caaaatgatg aggcatgatc ctctactcat tccaggaaat gagcagattg
     2821 agagcatgga tgctaatgtc aaacgatacg actctactgg aatgtttcac tggtgtccat
     2881 ccaaagaaat cgaaaaggtc attctgacac gcagcgaggc ctcaatgacg gttttaagtg
     2941 gacatgtagt agtctgcata tttggagatg tcacgtcagc tttagtgggg cttcgaaact
     3001 tagtcatgcc tttgagagca agcaacttcc attaccatga gctaaagccc attgtgtttg
     3061 tgggctcgtt agattatctg cgaagagagt gggaaaccct tcacaacttc cccaaagtct
     3121 tcatcttacc tggtacgcca ttaagtcgag ctgatttaag ggctgtcaac atcaacctct
     3181 gcgacatgtg cgttatcctg tcagccaatc agaacaatat cgacgatgct tcactgcagg
     3241 acaaggaatg catcttggca tctcttaaca tcaaatctat gcagtttgat gacagcattg
     3301 gggtcttgca ggctaattcc caaggtttca cacctccagg gatggatagg tcatctcctg
     3361 ataacagtcc cgtgcacggc ttagtgcggc aggcatccgt caccaccgga tcaaacatcc
     3421 ccatcataac tgaactggtg aacgactcca acgttcagtt tttggaccaa gacgacgatg
     3481 acgacccaga cactgagctc tacctgaccc agccgttcgc ctgtggtacc gccttcgccg
     3541 tcagcgtgtt ggactccctg atgagtgcga catacttcaa tgataatatc ctcaccctga
     3601 tccggacgct ggtcacggga ggcgccacgc cggagctgga ggccctgttg gctgaggaga
     3661 acgctctgag aggtggctac agcacgccgc agacgctggc taacagggac aggtgtcggg
     3721 tcgcccagct ggccctctac gatgggccct tcgcagactt gggggatgga ggttgctatg
     3781 gagacttgtt ctgtaaagca ttaaaaacct acaacatgct gtgctttgga atctatagat
     3841 tgcgagatgc acatttaggt gcaccaagcc agtgcacaaa aagatatgtc atcacaaacc
     3901 cgccttatga gtttgagatg gtgcccacgg acctgatctt ctgtctgatg cagttcgacc
     3961 acaacgcggg ccagtcccga gcgagcctgt cccactcctc ccattcgtcc cattcctcca
     4021 gcaagaagag ctcgtctgtt cactccatcc ccgccaccaa ccggcagaac cgcagcagca
     4081 aagcacgtga agcccgtgac aaacagaaaa aagagatggt ttacagatga agcagaaaac
     4141 gcatacccaa ggaacattca aatcaagcct atgagcactc acatggccaa ccaagtcaat
     4201 cagtacaaat ccactagcag tctgattcca cctatccgag aagtcgaaga cgaatgctga
     4261 aagggtgggc gcgccgaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     4321 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     4381 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     4441 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     4501 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     4561 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     4621 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     4681 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     4741 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     4801 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     4861 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     4921 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     4981 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     5041 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     5101 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     5161 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     5221 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     5281 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     5341 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     5401 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     5461 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5521 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5581 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5641 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5701 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5761 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5821 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5881 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5941 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6001 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6061 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6121 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6181 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6241 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6301 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg ga
//
