LOCUS       sequence-327046-       10030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of proneural Glioblastoma 
            cell-state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Schmitt MJ, Company C, Dramaretska Y, Barozzi I, Gohrig A, Kertalli 
            S, Grossmann M, Naumann H, Sanchez-Bailon MP, Hulsman D, Glass R, 
            Squatrito M, Serresi M, Gargiulo G
  TITLE     Phenotypic Mapping of Pathologic Cross-Talk between Glioblastoma and
            Innate Immune Cells by Synthetic Genetic Tracing.
  JOURNAL   Cancer Discov. 2021 Mar;11(3):754-777. doi: 
            10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23.
  PUBMED    33361384
REFERENCE   2  (bases 1 to 10030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-20-0219"; journalName: "Cancer Discov"; date: 
            "2021-03"; volume: "11"; issue: "3"; pages: "754-777"
COMMENT     Sequence Label: FH1-PNGT#2-mCherry
FEATURES             Location/Qualifiers
     source          1..10030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3530..3548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3566..3584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3634..4341
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3776..3794)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4044..4063
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4500..5088
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4553..4573)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4971..4982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5598..5778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5804..5821)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5810..6034
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6080..6508
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6167..6186)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6377..6398
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6517..6537)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6522..6851
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6702..6837
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6764..6783
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6899..6946
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6965..7339
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7469..7590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7506..7525)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7560..7579
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7639..7655)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7639..7655)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7652..7674)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7663..7679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7687..7693,7694..7711,7712..7717))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7732..7753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7870..7887)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8041..8629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8121..8140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8800..9591,9592..9660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9423..9442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9661..9765)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9840..9859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaatccctc ctctaatccc tccagcggga tcagggagga
     2401 ggtgcgggac ctgctgcccc gggcttgccc ccatcccggc ctcacgcatg ggcgcctgtc
     2461 tcagccctct cccaggacgc tgcaggtgtg gctgggccag cgctaattag tgggccgcgc
     2521 gggggccccg ctgagccttt gacagaaaag gcggtaggga ggtgggggca gggaggcgct
     2581 ccaccagcca gaagtccgga gcgcaaccca aagtactcca tctcaaaaga aaaaaggcgg
     2641 gggcggtggg gggggggggt gatttcagta caaagcctac agacattata aaaatattaa
     2701 gatttttgtt cgtttgtttt ttgtttttga gacagagtct cactgtcacc cccaggctgg
     2761 agtctgtgcc ggcgcccgct gcttcgcatc tgcgcgcccg cccggtgccg ggccccgccc
     2821 tccgcctcag ccccaagctc ggcccgcggg cccggccaca ggtgccccgg cggccccgcc
     2881 tggcccgagg gaagagggca gctgggaggg gcccatgaga gaaccaaaac tgtgccccca
     2941 ggcttggaaa gaaatcacat gtatggccag caggaaggtt ccggaaggtt ccggaggaca
     3001 cctgcaggtg ggactgagaa caggggtctc ggctgggagt ggctgaggcc atatgaggac
     3061 ctcgactgcc acaaacagct gcaggagtgt gacagatcac aacactagca ttgtacctca
     3121 aaatatgctt gtaccctaag gcacaagaac tggtttgact tacaaaactg atctcagagt
     3181 tgggatcaaa gtttttctac cactctacta tgagccctcg gccgggcccc gccccgccag
     3241 ctccgcgcgg ctctgggctc tctaggggtg gggctgcggg cggggccggc gcctaattgg
     3301 gccgcgggcg cctcgaggtg ggcggggcat aagggggcgg ggccgcggag accccgggcg
     3361 ggagtttttt tctgcaagcg agaggggggg tgttgttggt atcgccccct ccttctcctc
     3421 cccccagggg tgaaagtgca agaggaagtg cagccgctgc catctttcct ccgctccgaa
     3481 cacacggagc ccggggccgc acagccgccg ctcctgtaca ctagtttact ccctatcagt
     3541 gatagagaac gtatatccag tttactccct atcagtgata gagaattcat gtatggcagg
     3601 aagaagcgga gacagcgacg aagaaagctt ggggtgagca agggcgagga ggataacatg
     3661 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     3721 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     3781 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     3841 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     3901 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     3961 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     4021 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     4081 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     4141 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     4201 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     4261 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     4321 atggacgagc tatacaagta aaccggcggc atggacgagc tatacaagta aaccggtatc
     4381 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     4441 cctcccacac gtacaagtaa agcggccgcg atctacaatt cgatatcaag cttatcgata
     4501 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4561 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4621 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4681 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4741 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4801 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4861 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     4921 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4981 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5041 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgagacctag
     5101 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     5161 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     5221 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     5281 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     5341 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     5401 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     5461 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     5521 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     5581 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5641 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5701 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5761 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     5821 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5881 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5941 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6001 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6061 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6121 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6181 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6241 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6301 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6361 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6421 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6481 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     6541 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6601 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6661 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6721 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6781 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6841 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     6901 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     6961 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     7021 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     7081 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     7141 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     7201 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     7261 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     7321 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     7381 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7441 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     7501 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7561 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7621 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7681 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7741 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7801 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7861 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7921 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7981 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8161 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8221 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8281 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8341 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8401 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8461 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8521 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8581 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8641 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8701 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8761 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8821 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8881 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8941 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9001 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9061 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9121 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9181 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9241 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9301 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9361 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9421 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9481 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9541 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9601 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9661 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9721 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9781 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     9841 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     9901 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     9961 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
    10021 tatacgcgtt
//
