LOCUS       sequence-327047-        3272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish kcnmb2a gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pENTR-D-TOPO-kcnmb2a
FEATURES             Location/Qualifiers
     source          1..3272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     355..374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     608..625
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(784..811)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(903..989)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1038..1060
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1052..1069
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1053..1069
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1085..1184
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1913..2012)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2029..2048)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2030..2048)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2053..2069)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2053..2069)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2171..2190)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2182..2991
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2249..2268)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(3138..3272,1..454)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
       61 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
      121 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      181 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      241 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      301 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      361 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      421 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
      481 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      541 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      601 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
      661 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      721 gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac
      781 gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc
      841 gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc
      901 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
      961 tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac
     1021 gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg
     1081 gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga
     1141 gcaatgcttt tttataatgc caactttgta caaaaaagca ggctccgcgg ccgccccctt
     1201 caccatgttc tttgtgaagg gggtcagaac aacacaagcc acacaacaaa gggtgattta
     1261 taagcggttg tggggacatg agctgaagga caaaaaaagg acggagacgg cgctgaaggc
     1321 tggggaggac agggccattc tgctgggttt gagcatggtg ttcagctcag tcatgatgta
     1381 ctttgtgatt tgtatcacca tggtgcgtgc ttatgcagac agtctattga tcgatgagac
     1441 cacctgcact gtgctgaaca cctctctgac caaggaaatc aactgctcct acaactgtgg
     1501 ctctgactgc tggcgaagct cgaaataccc ctgtctgcag gtctacgtca gcctgaacgc
     1561 caccggtcgc atcggcctgc tctctcacaa tgaggaaatg ctggatgtca gttctgagtg
     1621 tttttatgtg ccaaaatgcc agaaggacca caccgccatg cgcgttctga tccagaatat
     1681 ttcggatcac ctgaagatgc agcagcaggt caggtgtttc cacgatccta gcgagcatca
     1741 agccagtatc ctcctgaagc ggctctacgg cagcgcttcg cttttacact cgctcttctg
     1801 gccatcctgc atgttatccg gaggaacgct catcatcctc atggtcaagc tcacgcaata
     1861 cctctccata ctgtgtgaac aaatcgacaa agccccaagg gtgggcgcgc cgacccagct
     1921 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     1981 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     2041 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     2101 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     2161 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     2221 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2281 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2341 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2401 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2461 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2521 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2581 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2641 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2701 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2761 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2821 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2881 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2941 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     3001 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     3061 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     3121 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3181 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3241 ccgaaggtaa ctggcttcag cagagcgcag at
//
