LOCUS       sequence-327050-       11621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of classical Glioblastoma 
            cell-state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Schmitt MJ, Company C, Dramaretska Y, Barozzi I, Gohrig A, Kertalli 
            S, Grossmann M, Naumann H, Sanchez-Bailon MP, Hulsman D, Glass R, 
            Squatrito M, Serresi M, Gargiulo G
  TITLE     Phenotypic Mapping of Pathologic Cross-Talk between Glioblastoma and
            Innate Immune Cells by Synthetic Genetic Tracing.
  JOURNAL   Cancer Discov. 2021 Mar;11(3):754-777. doi: 
            10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23.
  PUBMED    33361384
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-20-0219"; journalName: "Cancer Discov"; date: 
            "2021-03"; volume: "11"; issue: "3"; pages: "754-777"
COMMENT     Sequence Label: FH1-CLGT#2-mCherry-_PGK-H2B-CFP
FEATURES             Location/Qualifiers
     source          1..11621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3479..3497
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3515..3533
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3583..4290
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3725..3743)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3993..4012
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4449..5037
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4502..4522)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4920..4931)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5161..5671
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5590..5608
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5683..6060
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      6073..6082
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6079..6081,6082..6084,6085..6798)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6124..6145)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6385..6404)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6732..6753
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             7189..7369
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7395..7412)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7401..7625
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7671..8099
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7758..7777)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7968..7989
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8108..8128)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8113..8442
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8293..8428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8490..8537
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8556..8930
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9060..9181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9097..9116)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9151..9170
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9230..9246)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9230..9246)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9243..9265)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9254..9270
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9278..9284,9285..9302,9303..9308))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9323..9344
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9461..9478)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9632..10220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9712..9731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10391..11182,11183..11251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11014..11033
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11252..11356)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11431..11450)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaaagaata tctggctggc cacgtgtttg taaagaaaaa
     2401 ccaagacggc caggcgaggt ggctcacacc tgtaatccca gcactttggg aggccgaggc
     2461 gggcgctgcc cttcggcctt caaggaggaa ttcctactgt ttatgaagat cgggtttggg
     2521 tttttggttt tttttttctt tttctttttt ccgtggtggt ggtgggtggg cttttgttct
     2581 ttttgttttt tctgtggtgg tggtgggtgg gctttatgaa tataccatat tttgcctatt
     2641 gtttttctat ttatcaggtg gtgtcatttg agttgttttc accctcttgt gactatgaat
     2701 aatgatacta taaacaatct tatacagcat cagtgtcaaa aatcactaac attcctatac
     2761 acagacgtga ctaaacttcc agcttggggt cccgtggacc tgcagccagg tgcagcaggt
     2821 cacagggcaa ggacacgtgt cattggtgac cttcactatt cagtgcccag atgctcagtg
     2881 ctctgtgcag gccacctggc tggtctcagg taccgctgct ctgtctcgct caccggccgg
     2941 gctatgttga ttgtcccctc gcggcgcccg gaagcgaccc tcagtaaaca aagccgtgtg
     3001 tgggcgcagc cccagaagcc tggggcgcgc agtccagccc aagagaggcg ggggaggaat
     3061 gttgtgaatg aaccccgggc ccgccccgaa actccgcata aggcctgggc cgcgggggtc
     3121 ctcccactct gattggcctc tggcgccccg tgattgacag cgcccctcgc tgtgcgctct
     3181 ggttgggtaa acaagaaaag actggcatcg cagtcatcga gtgagcagcg aggcttggac
     3241 acgggtctgg cggcgcagcc aatggcgggg gagggccgag gaggccgagg gggggccaat
     3301 agggacaggc ggtgggggcg ggacgacggc ggagctaaag cggcggctga agcagcttca
     3361 ttgttgtgaa gagtcttaaa ggggccgcat caccctgccg gcccggcgcg ggtcgggggt
     3421 gggtgcggta ggggtcccgg ggcggccgag cgcagaggac ggatgtacac tagtttactc
     3481 cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag agaattcatg
     3541 tatggcagga agaagcggag acagcgacga agaaagcttg gggtgagcaa gggcgaggag
     3601 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     3661 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     3721 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     3781 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     3841 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     3901 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     3961 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     4021 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     4081 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     4141 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     4201 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     4261 accggcggca tggacgagct atacaagtaa accggcggca tggacgagct atacaagtaa
     4321 accggtatct agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc
     4381 tttaaaaaac ctcccacacg tacaagtaaa gcggccgcga tctacaattc gatatcaagc
     4441 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4501 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4561 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4621 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4681 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4741 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4801 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     4861 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4921 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4981 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     5041 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     5101 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     5161 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
     5221 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
     5281 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
     5341 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
     5401 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
     5461 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
     5521 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
     5581 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
     5641 cgttgaccga atcaccgacc tctctcccca gggggatcta ccatgccaga gccagcgaag
     5701 tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc
     5761 ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg
     5821 aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat gaactcgttt
     5881 gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag
     5941 cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag
     6001 ttggccaagc acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag
     6061 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     6121 atcctggtcg agctggacgg cgacgtaaac ggccacaggt tcagcgtgtc cggcgagggc
     6181 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     6241 cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc
     6301 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     6361 caggagcgta ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     6421 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     6481 ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc
     6541 gccgacaagc agaagaacgg catcaaggcc cacttcaaga tccgccacaa catcgaggac
     6601 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     6661 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     6721 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     6781 gacgagctgt acaagtaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6841 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     6901 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     6961 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     7021 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     7081 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     7141 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     7201 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7261 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7321 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
     7381 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     7441 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     7501 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     7561 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     7621 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     7681 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7741 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7801 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7861 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7921 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7981 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8041 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     8101 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     8161 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     8221 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     8281 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8341 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     8401 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     8461 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     8521 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     8581 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     8641 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     8701 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     8761 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     8821 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     8881 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     8941 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     9001 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     9061 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9121 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9181 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     9241 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9301 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9361 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9421 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9481 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9541 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9601 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9661 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9721 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9781 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9841 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9901 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9961 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10021 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    10081 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10141 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10201 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10261 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10321 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10381 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10441 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10501 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10561 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10621 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10681 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10741 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10801 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10861 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10921 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10981 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    11041 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    11101 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    11161 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    11221 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11281 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11341 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    11401 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
    11461 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
    11521 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
    11581 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt t
//
